Restriction map for
pFN21AA0330
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# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AclI AflII AscI AseI
AsnI AviII AvrII BbeI BbrPI
BfrI BlnI BsaAI BsiWI BspDI
BsrDI BssHII Bst1107I BstZ17I ClaI
CpoI EcoRI EcoRV FseI FspI
KasI KspI MluI NarI NdeI
NheI NotI NruI PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SexAI SfoI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AcsI 2974 GAATTT [GA]^AATT[CT]
AgeI 149 ACCGGT A^CCGGT
AhdI 5554 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2148 GTGCAC G^TGCAC
Aor51HI 744 AGCGCT AGC^GCT
ApaLI 2148 GTGCAC G^TGCAC
ApoI 2974 GAATTT [GA]^AATT[CT]
Asp700I 3378 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 5554 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 4670 GTATCC GTATCC(6/5)
BclI 45 TGATCA T^GATCA
BglII 4272 AGATCT A^GATCT
BsaBI 2373 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 3305 GCATTC GAATGC(1/-1)
BspHI 48 TCATGA T^CATGA
BstBI 5 TTCGAA TT^CGAA
DraI 61 TTTAAA TTT^AAA
DrdI 4930 GACCGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4780 CGGCCG C^GGCCG
Eam1105I 5554 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4780 CGGCCG C^GGCCG
Eco47III 744 AGCGCT AGC^GCT
Eco52I 4780 CGGCCG C^GGCCG
EcoT22I 3235 ATGCAT ATGCA^T
FbaI 45 TGATCA T^GATCA
HpaI 955 GTTAAC GTT^AAC
MamI 2373 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3478 CAATTG C^AATTG
MunI 3478 CAATTG C^AATTG
NsiI 3235 ATGCAT ATGCA^T
NspV 5 TTCGAA TT^CGAA
PinAI 149 ACCGGT A^CCGGT
RcaI 48 TCATGA T^CATGA
ScaI 2754 AGTACT AGT^ACT
SfuI 5 TTCGAA TT^CGAA
SmaI 3293 CCCGGG CCC^GGG
SspI 266 AATATT AAT^ATT
XmaCI 3293 CCCGGG C^CCGGG
XmaI 3293 CCCGGG C^CCGGG
XmnI 3378 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 2882 GGTACC G^GTACC
3117 GGTACC
AccI 1222 GTAGAC GT^[AC][GT]AC
1540 GTCTAC
AccIII 413 TCCGGA T^CCGGA
623 TCCGGA
AcyI 948 GACGCC G[GA]^CG[CT]C
2643 GACGCC
Asp718I 2882 GGTACC G^GTACC
3117 GGTACC
BamHI 416 GGATCC G^GATCC
4757 GGATCC
BcgI 194 CGAGCCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3714 CGAGGAGGCTGC
BsaHI 948 GACGCC G[GA]^CG[CT]C
2643 GACGCC
BseAI 413 TCCGGA T^CCGGA
623 TCCGGA
BsiEI 4116 CGACCG CG[GA][CT]^CG
4780 CGGCCG
BsmBI 2523 CGTCTC CGTCTC(1/5)
2641 GAGACG
BspEI 413 TCCGGA T^CCGGA
623 TCCGGA
BstEII 1771 GGTGACC G^GT[ACGT]ACC
6340 GGTCACC
BstPI 1771 GGTGACC G^GT[ACGT]ACC
6340 GGTCACC
EcoO65I 1771 GGTGACC G^GT[ACGT]ACC
6340 GGTCACC
Hin1I 948 GACGCC G[GA]^CG[CT]C
2643 GACGCC
KpnI 2882 GGTACC GGTAC^C
3117 GGTACC
MroI 413 TCCGGA T^CCGGA
623 TCCGGA
SapI 6046 GAAGAGC GCTCTTC(1/4)
4710 GCTCTTC
SphI 3360 GCATGC GCATG^C
3545 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspLU11I BspMI BsrBI BsrFI BsrGI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII