Restriction map for
pFN21AA0330
(Circular) (Six-base) MAPSORT of: pFN21AA0330.seq Check: 2977 from: 1 to: 11365
pFN21AA0330 11365 bp
With 182 enzymes: SgfI *
February 27, 2009 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4903 7675 7946 8104 8207 4903
Size: 2772 271 158 103 8061
Fragments arranged by size:
8061 2772 271 158 103
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9175 278
Size: 53 83 186 936 7639 2468
Fragments arranged by size:
7639 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3228 3546 8708 1937
Size: 1291 318 5162 4594
Fragments arranged by size:
5162 4594 1291 318
Acc65I G'GTAC_C
Cuts at: 4887 5122 8686 4887
Size: 235 3564 7566
Fragments arranged by size:
7566 3564 235
AclI AA'CG_TT
Cuts at: 1563 9492 9865 1563
Size: 7929 373 3063
Fragments arranged by size:
7929 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3884 4076 4274 4445 4518 4812 5051 5360
Size: 2252 192 198 171 73 294 239 309
Cuts at: 5360 5840 5919 5964 6266 6875 6893 8031 8540
Size: 480 79 45 302 609 18 1138 509
Cuts at: 8540 9428 10812 11080 1632
Size: 888 1384 268 1917
Fragments arranged by size:
2252 1917 1384 1138 888 609 509 480
309 302 294 268 239 198 192 171
79 73 45 18
AfeI AGC'GCT
Cuts at: 2751 2751
Size: 11365
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11168
AflIII A'CryG_T
Cuts at: 1203 2398 2531 2664 6657 10264 11241 1203
Size: 1195 133 133 3993 3607 977 1327
Fragments arranged by size:
3993 3607 1327 1195 977 133 133
AgeI A'CCGG_T
Cuts at: 2154 10196 2154
Size: 8042 3323
AhdI GACnn_n'nnGTC
Cuts at: 7564 10094 7564
Size: 2530 8835
AleI CACnn'nnGTG
Cuts at: 1166 3035 5048 1166
Size: 1869 2013 7483
Fragments arranged by size:
7483 2013 1869
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3593 3950 4517 5608 1184
Size: 2409 357 567 1091 6941
Fragments arranged by size:
6941 2409 1091 567 357
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4925 4957 4925
Size: 32 11333
AlwNI CAG_nnn'CTG
Cuts at: 3673 3907 3974 4081 4099 6392 6493 6946 7847
Size: 234 67 107 18 2293 101 453 901
Cuts at: 7847 8171 8189 8278 8518 9108 10680 3673
Size: 324 18 89 240 590 1572 4358
Fragments arranged by size:
4358 2293 1572 901 590 453 324 240
234 107 101 89 67 18 18
ApaI G_GGCC'C
Cuts at: 5355 5401 6002 6652 7032 7161 7275 7373 8042
Size: 46 601 650 380 129 114 98 669
Cuts at: 8042 5355
Size: 8678
Fragments arranged by size:
8678 669 650 601 380 129 114 98
46
ApaLI G'TGCA_C
Cuts at: 4153 9422 10578 4153
Size: 5269 1156 4940
Fragments arranged by size:
5269 4940 1156
ApoI r'AATT_y
Cuts at: 1493 4979 8674 8900 9052 11161 1493
Size: 3486 3695 226 152 2109 1697
Fragments arranged by size:
3695 3486 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9919 160
Size: 872 8887 1606
Fragments arranged by size:
8887 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11365
AvaI C'yCGr_G
Cuts at: 1870 1943 5298 7160 7373 7765 8690 1870
Size: 73 3355 1862 213 392 925 4545
Fragments arranged by size:
4545 3355 1862 925 392 213 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5106 5139 5106
Size: 33 11332
BamHI G'GATC_C
Cuts at: 2421 6762 8695 9062 2421
Size: 4341 1933 367 4724
Fragments arranged by size:
4724 4341 1933 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4071 4887 5122 5444 7834
Size: 325 217 627 2284 816 235 322 2390
Cuts at: 7834 8075 8686 10141 618
Size: 241 611 1455 1842
Fragments arranged by size:
2390 2284 1842 1455 816 627 611 325
322 241 235 217
BanII G_rGCy'C
Cuts at: 1391 2190 2871 4524 4738 5355 5401 5520 6002
Size: 799 681 1653 214 617 46 119 482
Cuts at: 6002 6192 6652 6717 7005 7032 7161 7262 7275
Size: 190 460 65 288 27 129 101 13
Cuts at: 7275 7284 7373 8042 8196 8285 8367 8684 1391
Size: 9 89 669 154 89 82 317 4072
Fragments arranged by size:
4072 1653 799 681 669 617 482 460
317 288 214 190 154 129 119 101
89 89 82 65 46 27 13 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4587 4800 6993 928
Size: 971 2688 213 2193 5300
Fragments arranged by size:
5300 2688 2193 971 213
BbvCI CC'TCA_GC
Cuts at: 3593 6415 6507 3593
Size: 2822 92 8451
Fragments arranged by size:
8451 2822 92
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2188 2222 5708 5742 9556 9590 1903
Size: 34 251 34 3486 34 3814 34 3678
Fragments arranged by size:
3814 3678 3486 251 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6686 9257 10467 6686
Size: 2571 1210 7584
Fragments arranged by size:
7584 2571 1210
BclI T'GATC_A
Cuts at: 1548 2050 1548
Size: 502 10863
BfrBI ATG'CAT
Cuts at: 5242 5242
Size: 11365
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2300 3367 5780
Size: 107 122 71 631 605 628 1067 2413
Cuts at: 5780 7107 7996 8005 9976 136
Size: 1327 889 9 1971 1525
Fragments arranged by size:
2413 1971 1525 1327 1067 889 631 628
605 122 107 71 9
BglII A'GATC_T
Cuts at: 6277 11360 6277
Size: 5083 6282
BlpI GC'TnA_GC
Cuts at: 3539 6995 7409 7532 8190 8782 3539
Size: 3456 414 123 658 592 6122
Fragments arranged by size:
6122 3456 658 592 414 123
Bme1580I G_kGCm'C
Cuts at: 1790 4157 5355 5401 6002 6652 7032 7161 7275
Size: 2367 1198 46 601 650 380 129 114
Cuts at: 7275 7373 7423 7839 8042 8078 8183 8332 9426
Size: 98 50 416 203 36 105 149 1094
Cuts at: 9426 10582 1790
Size: 1156 2573
Fragments arranged by size:
2573 2367 1198 1156 1094 650 601 416
380 203 149 129 114 105 98 50
46 36
BmgBI CAC'GTC
Cuts at: 1318 2876 3415 4528 5480 6658 1318
Size: 1558 539 1113 952 1178 6025
Fragments arranged by size:
6025 1558 1178 1113 952 539
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 7875 8536 9096
Size: 416 53 83 399 24 6458 661 560
Cuts at: 9096 10054 442
Size: 958 1753
Fragments arranged by size:
6458 1753 958 661 560 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11365
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4918 6029 6476 7540 8286 4918
Size: 1111 447 1064 746 7997
Fragments arranged by size:
7997 1111 1064 746 447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2342 3452 3479 3501 3577 3641 3857 3923
Size: 1103 1110 27 22 76 64 216 66
Cuts at: 3923 3929 4055 4085 4103 4205 4748 4841 4913
Size: 6 126 30 18 102 543 93 72
Cuts at: 4913 5142 5423 5807 6196 6626 7421 7574 7727
Size: 229 281 384 389 430 795 153 153
Cuts at: 7727 8609 8618 8645 10025 1239
Size: 882 9 27 1380 2579
Fragments arranged by size:
2579 1380 1110 1103 882 795 543 430
389 384 281 229 216 153 153 126
102 93 76 72 66 64 30 27
27 22 18 9 6
Bpu10I CC'TnA_GC
Cuts at: 2097 3593 5075 5514 5766 6415 6507 6563 7147
Size: 1496 1482 439 252 649 92 56 584
Cuts at: 7147 8226 8250 8279 8361 2097
Size: 1079 24 29 82 5101
Fragments arranged by size:
5101 1496 1482 1079 649 584 439 252
92 82 56 29 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2579 2954 6818 9490 10355 10653 2579
Size: 375 3864 2672 865 298 3291
Fragments arranged by size:
3864 3291 2672 865 375 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3028 7658 8498 10028 882
Size: 617 1529 4630 840 1530 2219
Fragments arranged by size:
4630 2219 1530 1529 840 617
BsaAI yAC'GTr
Cuts at: 493 1204 9156 493
Size: 711 7952 2702
Fragments arranged by size:
7952 2702 711
BsaBI GATnn'nnATC
Cuts at: 4382 11359 4382
Size: 6977 4388
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2954 4649 9172 9554
Size: 53 83 186 936 1421 1695 4523 382
Cuts at: 9554 10189 275
Size: 635 1451
Fragments arranged by size:
4523 1695 1451 1421 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2154 2225 2418 2628 4560 6633 9798 10196 10470
Size: 71 193 210 1932 2073 3165 398 274
Cuts at: 10470 10617 11028 2154
Size: 147 411 2491
Fragments arranged by size:
3165 2491 2073 1932 411 398 274 210
193 147 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3073 3103 5115 5145 5954
Size: 30 165 30 1670 30 2012 30 809
Cuts at: 5954 5984 6046 6076 7161 7191 7927 7957 1178
Size: 30 62 30 1085 30 736 30 4586
Fragments arranged by size:
4586 2012 1670 1085 809 736 165 62
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2177 2627 3302 3574 3696 4052 4100 4361
Size: 991 450 675 272 122 356 48 261
Cuts at: 4361 4523 4829 5627 5630 5735 5927 6010 6013
Size: 162 306 798 3 105 192 83 3
Cuts at: 6013 6329 6524 7175 7418 7529 7709 8516 1186
Size: 316 195 651 243 111 180 807 4035
Fragments arranged by size:
4035 991 807 798 675 651 450 356
316 306 272 261 243 195 192 180
162 122 111 105 83 48 3 3
BseYI C'CCAG_C
Cuts at: 2295 2307 2879 2921 2995 3807 4218 4624 4865
Size: 12 572 42 74 812 411 406 241
Cuts at: 4865 4908 5638 5984 6385 6576 6846 7116 7447
Size: 43 730 346 401 191 270 270 331
Cuts at: 7447 7452 8021 8315 8331 8366 10568 11064 2295
Size: 5 569 294 16 35 2202 496 2596
Fragments arranged by size:
2596 2202 812 730 572 569 496 411
406 401 346 331 294 270 270 241
191 74 43 42 35 16 12 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2712 2810 3044 3910 4523 5543 5672
Size: 573 976 98 234 866 613 1020 129
Cuts at: 5672 5699 5874 7245 7893 1163
Size: 27 175 1371 648 4635
Fragments arranged by size:
4635 1371 1020 976 866 648 613 573
234 175 129 98 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6124 6788 8815 9576 10604 1540
Size: 275 186 4123 664 2027 761 1028 2301
Fragments arranged by size:
4123 2301 2027 1028 761 664 275 186
BsiHKAI G_wGCw'C
Cuts at: 2190 3540 4157 5520 6984 7005 8285 9426 9511
Size: 1350 617 1363 1464 21 1280 1141 85
Cuts at: 9511 10582 2190
Size: 1071 2973
Fragments arranged by size:
2973 1464 1363 1350 1280 1141 1071 617
85 21
BsmI GAATG_Cn'
Cuts at: 5310 8876 8969 5310
Size: 3566 93 7706
Fragments arranged by size:
7706 3566 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4534 4640 4534
Size: 106 11259
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2190 2871 3540 4157 4524 4738 5355
Size: 399 400 681 669 617 367 214 617
Cuts at: 5355 5401 5520 6002 6192 6652 6717 6984 7005
Size: 46 119 482 190 460 65 267 21
Cuts at: 7005 7032 7161 7262 7275 7284 7373 7423 7839
Size: 27 129 101 13 9 89 50 416
Cuts at: 7839 8042 8078 8183 8196 8285 8332 8367 8684
Size: 203 36 105 13 89 47 35 317
Cuts at: 8684 9426 9511 10582 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 681 669 617 617 482
460 416 400 399 367 317 267 214
203 190 129 119 105 101 89 89
85 65 50 47 46 36 35 27
21 13 13 9
BspEI T'CCGG_A
Cuts at: 2418 2628 11028 2418
Size: 210 8400 2755
Fragments arranged by size:
8400 2755 210
BspHI T'CATG_A
Cuts at: 2053 9254 10984 2053
Size: 7201 1730 2434
Fragments arranged by size:
7201 2434 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4903 5721 7675 7946 8104 8207 8720 844
Size: 4059 818 1954 271 158 103 513 3489
Fragments arranged by size:
4059 3489 1954 818 513 271 158 103
BsrBI CCG'CTC
Cuts at: 1394 1823 4069 6816 7243 7327 7441 7522 9252
Size: 429 2246 2747 427 84 114 81 1730
Cuts at: 9252 1394
Size: 3507
Fragments arranged by size:
3507 2747 2246 1730 429 427 114 84
81
BsrDI GCAATG_nn'
Cuts at: 65 1248 9860 10034 65
Size: 1183 8612 174 1396
Fragments arranged by size:
8612 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2154 2571 5538 6496 6703 6783 7194
Size: 110 344 417 2967 958 207 80 411
Cuts at: 7194 7380 8161 10009 10196 1700
Size: 186 781 1848 187 2869
Fragments arranged by size:
2967 2869 1848 958 781 417 411 344
207 187 186 110 80
BsrGI T'GTAC_A
Cuts at: 96 2508 2844 4854 96
Size: 2412 336 2010 6607
Fragments arranged by size:
6607 2412 2010 336
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11365
BssSI C'ACGA_G
Cuts at: 4691 5519 5717 7291 9425 10437 4691
Size: 828 198 1574 2134 1012 5619
Fragments arranged by size:
5619 2134 1574 1012 828 198
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3415 6691 6700 7916 8171 794
Size: 2621 3276 9 1216 255 3988
Fragments arranged by size:
3988 3276 2621 1216 255 9
BstBI TT'CG_AA
Cuts at: 2011 10169 2011
Size: 8158 3207
BstEII G'GTnAC_C
Cuts at: 1184 3776 8345 1184
Size: 2592 4569 4204
Fragments arranged by size:
4569 4204 2592
BstXI CCAn_nnnn'nTGG
Cuts at: 3394 3961 4183 4891 6202 8157 3394
Size: 567 222 708 1311 1955 6602
Fragments arranged by size:
6602 1955 1311 708 567 222
BstYI r'GATC_y
Cuts at: 1972 2317 2421 4918 5745 6277 6762 8695 9062
Size: 345 104 2497 827 532 485 1933 367
Cuts at: 9062 9447 9464 10916 11002 11014 11360 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2497 1977 1933 1452 827 532 485 385
367 346 345 104 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2408 3285 7092 8091 2408
Size: 877 3807 999 5682
Fragments arranged by size:
5682 3807 999 877
BtgI C'CryG_G
Cuts at: 513 1065 2331 2943 3486 5775 6680 6768 7206
Size: 552 1266 612 543 2289 905 88 438
Cuts at: 7206 7262 7284 8099 513
Size: 56 22 815 3779
Fragments arranged by size:
3779 2289 1266 905 815 612 552 543
438 88 56 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6526 487
Size: 683 56 5300 5326
Fragments arranged by size:
5326 5300 683 56
BtsI GCAGTG_nn'
Cuts at: 2358 3056 3705 3766 4274 4417 4844 5498 5990
Size: 698 649 61 508 143 427 654 492
Cuts at: 5990 6542 8152 8326 8881 9675 9695 2358
Size: 552 1610 174 555 794 20 4028
Fragments arranged by size:
4028 1610 794 698 654 649 555 552
508 492 427 174 143 61 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11365
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4432 4467 6049 6084 7021 7056 529
Size: 35 3868 35 1582 35 937 35 4838
Fragments arranged by size:
4838 3868 1582 937 35 35 35 35
DraI TTT'AAA
Cuts at: 2068 8669 2068
Size: 6601 4764
DraIII CAC_nnn'GTG
Cuts at: 3207 4152 6435 3207
Size: 945 2283 8137
Fragments arranged by size:
8137 2283 945
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6941 10372 809
Size: 507 5625 3431 1802
Fragments arranged by size:
5625 3431 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2838 4356 5019
Size: 54 1425 50 275 18 1008 1518 663
Cuts at: 5019 5704 5772 6785 7099 7203 7997 8159 8812
Size: 685 68 1013 314 104 794 162 653
Cuts at: 8812 9701 11330 8
Size: 889 1629 43
Fragments arranged by size:
1629 1518 1425 1013 1008 889 794 685
663 653 314 275 162 104 68 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6785 8812 1537
Size: 275 4973 2027 4090
Fragments arranged by size:
4973 4090 2027 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3150 3213 3976 4537 4868 5027 5336 5983
Size: 1797 63 763 561 331 159 309 647
Cuts at: 5983 6227 6443 6722 8046 9295 1353
Size: 244 216 279 1324 1249 3423
Fragments arranged by size:
3423 1797 1324 1249 763 647 561 331
309 279 244 216 159 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3542 4130 6542 6775 8410 9952
Size: 1064 138 1953 588 2412 233 1635 1542
Cuts at: 9952 10326 10472 387
Size: 374 146 1280
Fragments arranged by size:
2412 1953 1635 1542 1280 1064 588 374
233 146 138
EcoICRI GAG'CTC
Cuts at: 2188 5518 7003 8283 2188
Size: 3330 1485 1280 5270
Fragments arranged by size:
5270 3330 1485 1280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2342 3452 3479 3501 3577 3641 3857
Size: 393 710 1110 27 22 76 64 216
Cuts at: 3857 3884 3923 3929 4055 4076 4085 4103 4205
Size: 27 39 6 126 21 9 18 102
Cuts at: 4205 4274 4445 4518 4748 4812 4841 4913 5051
Size: 69 171 73 230 64 29 72 138
Cuts at: 5051 5142 5360 5423 5807 5840 5919 5964 6196
Size: 91 218 63 384 33 79 45 232
Cuts at: 6196 6266 6626 6875 6893 7421 7574 7727 8031
Size: 70 360 249 18 528 153 153 304
Cuts at: 8031 8540 8609 8618 8645 9428 10025 10812 11080
Size: 509 69 9 27 783 597 787 268
Cuts at: 11080 1239
Size: 1524
Fragments arranged by size:
1524 1110 787 783 710 597 528 509
393 384 360 304 268 249 232 230
218 216 171 153 153 138 126 102
91 79 76 73 72 70 69 69
64 64 63 45 39 33 29 27
27 27 22 21 18 18 9 9
6
EcoNI CCTnn'n_nnAGG
Cuts at: 3821 4475 5135 5717 5902 6872 3821
Size: 654 660 582 185 970 8314
Fragments arranged by size:
8314 970 660 654 582 185
EcoO109I rG'GnC_Cy
Cuts at: 2131 2917 3762 3904 4393 4710 5398 5782 5860
Size: 786 845 142 489 317 688 384 78
Cuts at: 5860 5894 5999 6457 6943 7028 7108 7157 7271
Size: 34 105 458 486 85 80 49 114
Cuts at: 7271 7369 7370 7981 8311 8593 2131
Size: 98 1 611 330 282 4903
Fragments arranged by size:
4903 845 786 688 611 489 486 458
384 330 317 282 142 114 105 98
85 80 78 49 34 1
EcoRI G'AATT_C
Cuts at: 8674 9052 8674
Size: 378 10987
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3889 3889
Size: 11365
FspI TGC'GCA
Cuts at: 9871 9871
Size: 11365
HaeII r_GCGC'y
Cuts at: 1678 2753 3090 3884 8190 10512 1678
Size: 1075 337 794 4306 2322 2531
Fragments arranged by size:
4306 2531 2322 1075 794 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3073 3105 3975 4007 5075
Size: 32 135 32 1501 32 870 32 1068
Cuts at: 5075 5107 5954 5960 5986 5992 6149 6181 7550
Size: 32 847 6 26 6 157 32 1369
Cuts at: 7550 7582 10006 10038 10080 10112 1373
Size: 32 2424 32 42 32 2626
Fragments arranged by size:
2626 2424 1501 1369 1068 870 847 157
135 42 32 32 32 32 32 32
32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2377 2962 5869 8709 8955 669
Size: 988 281 439 585 2907 2840 246 3079
Fragments arranged by size:
3079 2907 2840 988 585 439 281 246
HindIII A'AGCT_T
Cuts at: 748 2062 2553 3343 5931 748
Size: 1314 491 790 2588 6182
Fragments arranged by size:
6182 2588 1314 790 491
HpaI GTT'AAC
Cuts at: 2962 8955 2962
Size: 5993 5372
KpnI G_GTAC'C
Cuts at: 4891 5126 8690 4891
Size: 235 3564 7566
Fragments arranged by size:
7566 3564 235
MfeI C'AATT_G
Cuts at: 5483 8964 5483
Size: 3481 7884
MluI A'CGCG_T
Cuts at: 11241 11241
Size: 11365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2886 3057 5442 7566 7641 10479 10663 1305
Size: 1581 171 2385 2124 75 2838 184 2007
Fragments arranged by size:
2838 2385 2124 2007 1581 184 171 75
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2840 4358 5021 5706 5774
Size: 54 1425 343 1008 1518 663 685 68
Cuts at: 5774 7101 7205 10
Size: 1327 104 4170
Fragments arranged by size:
4170 1518 1425 1327 1008 685 663 343
104 68 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2948 3035 3392 3932 3959 4499 5048
Size: 648 1782 87 357 540 27 540 549
Cuts at: 5048 9323 9682 9841 518
Size: 4275 359 159 2042
Fragments arranged by size:
4275 2042 1782 648 549 540 540 359
357 159 87 27
MspA1I CmG'CkG
Cuts at: 1606 2299 2311 2696 2999 3555 3589 3661 3790
Size: 693 12 385 303 556 34 72 129
Cuts at: 3790 4096 4222 4628 5592 5984 6517 6850 6895
Size: 306 126 406 964 392 533 333 45
Cuts at: 6895 6955 7862 7876 8297 8781 9458 10606 10851
Size: 60 907 14 421 484 677 1148 245
Cuts at: 10851 1606
Size: 2120
Fragments arranged by size:
2120 1148 964 907 693 677 556 533
484 421 406 392 385 333 306 303
245 129 126 72 60 45 34 14
12
NaeI GCC'GGC
Cuts at: 5540 6498 6705 7382 8163 5540
Size: 958 207 677 781 8742
Fragments arranged by size:
8742 958 781 677 207
NcoI C'CATG_G
Cuts at: 513 1065 2331 2943 3486 5775 7206 7284 513
Size: 552 1266 612 543 2289 1431 78 4594
Fragments arranged by size:
4594 2289 1431 1266 612 552 543 78
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11365
NgoMIV G'CCGG_C
Cuts at: 5538 6496 6703 7380 8161 5538
Size: 958 207 677 781 8742
Fragments arranged by size:
8742 958 781 677 207
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11365
NotI GC'GGCC_GC
Cuts at: 8812 8812
Size: 11365
NsiI A_TGCA'T
Cuts at: 5244 5244
Size: 11365
NspI r_CATG'y
Cuts at: 1132 1229 2402 2535 2668 5242 5369 5554 5638
Size: 97 1173 133 133 2574 127 185 84
Cuts at: 5638 6703 7675 8401 8723 10268 1132
Size: 1065 972 726 322 1545 2229
Fragments arranged by size:
2574 2229 1545 1173 1065 972 726 322
185 133 133 127 97 84
PasI CC'CwG_GG
Cuts at: 1348 2255 3758 6464 6487 7025 7985 8176 1348
Size: 907 1503 2706 23 538 960 191 4537
Fragments arranged by size:
4537 2706 1503 960 907 538 191 23
PciI A'CATG_T
Cuts at: 2398 2531 2664 10264 2398
Size: 133 133 7600 3499
Fragments arranged by size:
7600 3499 133 133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3356 3772 4081 4315 4915 6435 6946 1108
Size: 2248 416 309 234 600 1520 511 5527
Fragments arranged by size:
5527 2248 1520 600 511 416 309 234
PfoI T'CCnGG_A
Cuts at: 3234 3456 6212 7551 8595 3234
Size: 222 2756 1339 1044 6004
Fragments arranged by size:
6004 2756 1339 1044 222
PmeI GTTT'AAAC
Cuts at: 8669 8669
Size: 11365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 9434 9466 10947 10979 9434
Size: 32 1481 32 9820
Fragments arranged by size:
9820 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2917 3762 4710 5860 5894 6457 6943 7981 8593
Size: 845 948 1150 34 563 486 1038 612
Cuts at: 8593 2917
Size: 5689
Fragments arranged by size:
5689 1150 1038 948 845 612 563 486
34
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11365
PsiI TTA'TAA
Cuts at: 2283 8935 2283
Size: 6652 4713
PspOMI G'GGCC_C
Cuts at: 5351 5397 5998 6648 7028 7157 7271 7369 8038
Size: 46 601 650 380 129 114 98 669
Cuts at: 8038 5351
Size: 8678
Fragments arranged by size:
8678 669 650 601 380 129 114 98
46
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5159 5191 5159
Size: 32 11333
PstI C_TGCA'G
Cuts at: 830 2145 3605 3700 3888 4236 4493 5890 6352
Size: 1315 1460 95 188 348 257 1397 462
Cuts at: 6352 6379 6696 8204 8717 830
Size: 27 317 1508 513 3478
Fragments arranged by size:
3478 1508 1460 1397 1315 513 462 348
317 257 188 95 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11365
PvuII CAG'CTG
Cuts at: 2299 2696 2999 3555 3661 3790 4222 5984 6850
Size: 397 303 556 106 129 432 1762 866
Cuts at: 6850 7862 8297 2299
Size: 1012 435 5367
Fragments arranged by size:
5367 1762 1012 866 556 435 432 397
303 129 106
SacI G_AGCT'C
Cuts at: 2190 5520 7005 8285 2190
Size: 3330 1485 1280 5270
Fragments arranged by size:
5270 3330 1485 1280
SalI G'TCGA_C
Cuts at: 1936 8707 1936
Size: 6771 4594
SanDI GG'GwC_CC
Cuts at: 3762 3762
Size: 11365
SapI GCTCTTCn'nnn_
Cuts at: 6722 8046 6722
Size: 1324 10041
SbfI CC_TGCA'GG
Cuts at: 8717 8717
Size: 11365
ScaI AGT'ACT
Cuts at: 4761 9613 11325 4761
Size: 4852 1712 4801
Fragments arranged by size:
4852 4801 1712
SfcI C'TryA_G
Cuts at: 826 1045 2141 3601 3696 3785 3884 4232 4489
Size: 219 1096 1460 95 89 99 348 257
Cuts at: 4489 5692 5886 6348 6375 6619 6692 8200 8713
Size: 1203 194 462 27 244 73 1508 513
Cuts at: 8713 9848 10529 10720 826
Size: 1135 681 191 1471
Fragments arranged by size:
1508 1471 1460 1203 1135 1096 681 513
462 348 257 244 219 194 191 99
95 89 73 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 5780 7107 7996 8005 5780
Size: 1327 889 9 9140
Fragments arranged by size:
9140 1327 889 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11365
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11365
SmaI CCC'GGG
Cuts at: 1872 5300 8692 1872
Size: 3428 3392 4545
Fragments arranged by size:
4545 3428 3392
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2594 2933 6833 9469 10370 10632
Size: 197 926 651 339 3900 2636 901 262
Cuts at: 10632 820
Size: 1553
Fragments arranged by size:
3900 2636 1553 926 901 651 339 262
197
SnaBI TAC'GTA
Cuts at: 493 9156 493
Size: 8663 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11365
SphI G_CATG'C
Cuts at: 1132 5369 5554 8723 1132
Size: 4237 185 3169 3774
Fragments arranged by size:
4237 3774 3169 185
SrfI GCCC'GGGC
Cuts at: 5300 5300
Size: 11365
SspI AAT'ATT
Cuts at: 5 52 2273 9289 5
Size: 47 2221 7016 2081
Fragments arranged by size:
7016 2221 2081 47
StuI AGG'CCT
Cuts at: 1517 2723 5951 7763 8008 8224 1517
Size: 1206 3228 1812 245 216 4658
Fragments arranged by size:
4658 3228 1812 1206 245 216
StyI C'CwwG_G
Cuts at: 513 1065 2331 2724 2884 2943 3486 4341 4515
Size: 552 1266 393 160 59 543 855 174
Cuts at: 4515 4830 5220 5775 7032 7206 7284 7977 8307
Size: 315 390 555 1257 174 78 693 330
Cuts at: 8307 8804 513
Size: 497 3074
Fragments arranged by size:
3074 1266 1257 855 693 555 552 543
497 393 390 330 315 174 174 160
78 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3086 3764 4342 5686 5941 6138 6442
Size: 1255 1506 678 578 1344 255 197 304
Cuts at: 6442 8364 8476 9392 9409 9562 9747 10192 325
Size: 1922 112 916 17 153 185 445 1498
Fragments arranged by size:
1922 1506 1498 1344 1255 916 678 578
445 304 255 197 185 153 112 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2508 2538 2844
Size: 274 80 33 51 1443 531 30 306
Cuts at: 2844 4759 4854 5793 6329 9611 11323 96
Size: 1915 95 939 536 3282 1712 138
Fragments arranged by size:
3282 1915 1712 1443 939 536 531 306
274 138 95 80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6046 6078 7161 7193 7927 7959 11325 11357 6046
Size: 32 1083 32 734 32 3366 32 6054
Fragments arranged by size:
6054 3366 1083 734 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3989 5087 7868 1316
Size: 2673 1098 2781 4813
Fragments arranged by size:
4813 2781 2673 1098
XbaI T'CTAG_A
Cuts at: 8701 8701
Size: 11365
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2998 5782 5981 6199 7282 7736 2998
Size: 2784 199 218 1083 454 6627
Fragments arranged by size:
6627 2784 1083 454 218 199
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11365
XmaI C'CCGG_G
Cuts at: 1870 5298 8690 1870
Size: 3428 3392 4545
Fragments arranged by size:
4545 3428 3392
XmnI GAAnn'nnTTC
Cuts at: 5387 9494 5387
Size: 4107 7258
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9173 276
Size: 53 83 186 936 7639 2468
Fragments arranged by size:
7639 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BbeI BsiWI BstZ17I EcoRV FseI FspAI
KasI NarI NruI PacI PmlI PspXI RsrII SacII
SexAI SfoI SwaI