(Circular) (Six-base) MAPSORT of: pFN21AA0082.seq Check: 6000 from: 1 to: 7210 pFN21AA0082 7210 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2455 2455 Size: 7210 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3435 4482 5020 278 Size: 53 83 186 936 1899 1047 538 2468 Fragments arranged by size: 2468 1899 1047 936 538 186 83 53 AccI GT'mk_AC Cuts at: 1937 3255 4553 1937 Size: 1318 1298 4594 Fragments arranged by size: 4594 1318 1298 Acc65I G'GTAC_C Cuts at: 4531 4531 Size: 7210 AclI AA'CG_TT Cuts at: 1563 5337 5710 1563 Size: 3774 373 3063 Fragments arranged by size: 3774 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2287 3371 3697 3905 5273 6657 6925 1632 Size: 655 1084 326 208 1368 1384 268 1917 Fragments arranged by size: 1917 1384 1368 1084 655 326 268 208 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7013 AflIII A'CryG_T Cuts at: 1203 6109 7086 1203 Size: 4906 977 1327 Fragments arranged by size: 4906 1327 977 AgeI A'CCGG_T Cuts at: 6041 6041 Size: 7210 AhdI GACnn_n'nnGTC Cuts at: 4465 5939 4465 Size: 1474 5736 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7210 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3167 4445 1184 Size: 1983 1278 3949 Fragments arranged by size: 3949 1983 1278 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3410 3442 3410 Size: 32 7178 AlwNI CAG_nnn'CTG Cuts at: 2419 4953 6525 2419 Size: 2534 1572 3104 Fragments arranged by size: 3104 2534 1572 ApaI G_GGCC'C Cuts at: 2918 2918 Size: 7210 ApaLI G'TGCA_C Cuts at: 5267 6423 5267 Size: 1156 6054 ApoI r'AATT_y Cuts at: 1493 2496 3043 3476 4041 4058 4519 4745 4897 Size: 1003 547 433 565 17 461 226 152 Cuts at: 4897 7006 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1003 565 547 461 433 226 152 17 AseI AT'TA_AT Cuts at: 160 1032 5764 160 Size: 872 4732 1606 Fragments arranged by size: 4732 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7210 AvaI C'yCGr_G Cuts at: 1870 1943 2661 3900 4535 1870 Size: 73 718 1239 635 4545 Fragments arranged by size: 4545 1239 718 635 73 BamHI G'GATC_C Cuts at: 3624 4540 4907 3624 Size: 916 367 5927 Fragments arranged by size: 5927 916 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4007 4190 4531 5986 618 Size: 325 217 627 2220 183 341 1455 1842 Fragments arranged by size: 2220 1842 1455 627 341 325 217 183 BanII G_rGCy'C Cuts at: 1391 2451 2656 2918 4184 4275 4529 1391 Size: 1060 205 262 1266 91 254 4072 Fragments arranged by size: 4072 1266 1060 262 254 205 91 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2686 3893 928 Size: 971 787 1207 4245 Fragments arranged by size: 4245 1207 971 787 BbvCI CC'TCA_GC Cuts at: 2084 3140 2084 Size: 1056 6154 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5401 5435 1903 Size: 34 3464 34 3678 Fragments arranged by size: 3678 3464 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5102 6312 5102 Size: 1210 6000 BclI T'GATC_A Cuts at: 1548 3459 1548 Size: 1911 5299 BfrBI ATG'CAT Cuts at: 2213 2213 Size: 7210 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2847 4087 5821 Size: 107 122 71 631 605 1175 1240 1734 Cuts at: 5821 136 Size: 1525 Fragments arranged by size: 1734 1525 1240 1175 631 605 122 107 71 BglII A'GATC_T Cuts at: 3834 7205 3834 Size: 3371 3839 BlpI GC'TnA_GC Cuts at: 2808 4202 4627 2808 Size: 1394 425 5391 Fragments arranged by size: 5391 1394 425 Bme1580I G_kGCm'C Cuts at: 1790 2918 3698 5271 6427 1790 Size: 1128 780 1573 1156 2573 Fragments arranged by size: 2573 1573 1156 1128 780 BmgBI CAC'GTC Cuts at: 1318 2094 1318 Size: 776 6434 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2445 4941 5899 Size: 416 53 83 399 24 1028 2496 958 Cuts at: 5899 442 Size: 1753 Fragments arranged by size: 2496 1753 1028 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7210 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3046 3305 3597 3046 Size: 259 292 6659 Fragments arranged by size: 6659 292 259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2954 3473 3770 4168 5870 1239 Size: 1715 519 297 398 1702 2579 Fragments arranged by size: 2579 1715 1702 519 398 297 Bpu10I CC'TnA_GC Cuts at: 2078 2084 3140 2078 Size: 6 1056 6148 Fragments arranged by size: 6148 1056 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5335 6200 6498 5335 Size: 865 298 6047 Fragments arranged by size: 6047 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2165 3462 5873 882 Size: 617 666 1297 2411 2219 Fragments arranged by size: 2411 2219 1297 666 617 BsaAI yAC'GTr Cuts at: 493 1204 5001 493 Size: 711 3797 2702 Fragments arranged by size: 3797 2702 711 BsaBI GATnn'nnATC Cuts at: 3707 4169 7204 3707 Size: 462 3035 3713 Fragments arranged by size: 3713 3035 462 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3432 4479 5017 5399 Size: 53 83 186 936 1899 1047 538 382 Cuts at: 5399 6034 275 Size: 635 1451 Fragments arranged by size: 1899 1451 1047 936 635 538 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3189 5643 6041 6315 6462 6873 3189 Size: 2454 398 274 147 411 3526 Fragments arranged by size: 3526 2454 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4469 4499 1178 Size: 30 165 30 3066 30 3889 Fragments arranged by size: 3889 3066 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 2104 2703 1186 Size: 840 78 599 5693 Fragments arranged by size: 5693 840 599 78 BseYI C'CCAG_C Cuts at: 6413 6909 6413 Size: 496 6714 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6637 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4660 5421 6449 1540 Size: 275 186 2659 761 1028 2301 Fragments arranged by size: 2659 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2656 5271 5356 6427 2656 Size: 2615 85 1071 3439 Fragments arranged by size: 3439 2615 1071 85 BsmI GAATG_Cn' Cuts at: 2033 2213 4422 4721 4814 2033 Size: 180 2209 299 93 4429 Fragments arranged by size: 4429 2209 299 180 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2278 2278 Size: 7210 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2451 2656 2918 3698 4184 4275 4529 Size: 399 661 205 262 780 486 91 254 Cuts at: 4529 5271 5356 6427 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 780 742 661 486 399 262 254 205 91 85 BspEI T'CCGG_A Cuts at: 3189 6873 3189 Size: 3684 3526 BspHI T'CATG_A Cuts at: 5099 6829 5099 Size: 1730 5480 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2455 3135 3267 4565 844 Size: 1611 680 132 1298 3489 Fragments arranged by size: 3489 1611 1298 680 132 BsrBI CCG'CTC Cuts at: 1394 1823 5097 1394 Size: 429 3274 3507 Fragments arranged by size: 3507 3274 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2721 4365 5705 5879 65 Size: 1183 1473 1644 1340 174 1396 Fragments arranged by size: 1644 1473 1396 1340 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4224 4395 5854 6041 1700 Size: 110 2414 171 1459 187 2869 Fragments arranged by size: 2869 2414 1459 187 171 110 BsrGI T'GTAC_A Cuts at: 96 4122 96 Size: 4026 3184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7210 BssSI C'ACGA_G Cuts at: 3639 5270 6282 3639 Size: 1631 1012 4567 Fragments arranged by size: 4567 1631 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2072 794 Size: 1278 5932 BstBI TT'CG_AA Cuts at: 2967 6014 2967 Size: 3047 4163 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7210 BstYI r'GATC_y Cuts at: 1972 3135 3624 3756 3834 4540 4907 5292 5309 Size: 1163 489 132 78 706 367 385 17 Cuts at: 5309 6761 6847 6859 7205 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1163 706 489 385 367 346 132 86 78 17 12 BtgI C'CryG_G Cuts at: 513 1065 2765 4239 513 Size: 552 1700 1474 3484 Fragments arranged by size: 3484 1700 1474 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6471 Fragments arranged by size: 6471 683 56 BtsI GCAGTG_nn' Cuts at: 2031 2612 3396 3728 3802 4726 5520 5540 2031 Size: 581 784 332 74 924 794 20 3701 Fragments arranged by size: 3701 924 794 784 581 332 74 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7210 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7175 DraI TTT'AAA Cuts at: 4514 4514 Size: 7210 DraIII CAC_nnn'GTG Cuts at: 2599 3575 2599 Size: 976 6234 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6217 809 Size: 507 4901 1802 Fragments arranged by size: 4901 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2289 3850 4393 Size: 54 1425 50 275 18 459 1561 543 Cuts at: 4393 4657 5546 7175 8 Size: 264 889 1629 43 Fragments arranged by size: 1629 1561 1425 889 543 459 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4657 1537 Size: 275 2845 4090 Fragments arranged by size: 4090 2845 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2003 2374 2577 2634 2969 3296 3389 4128 Size: 650 371 203 57 335 327 93 739 Cuts at: 4128 4406 5140 1353 Size: 278 734 3423 Fragments arranged by size: 3423 739 734 650 371 335 327 278 203 93 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3749 5797 6171 6317 387 Size: 1064 138 2160 2048 374 146 1280 Fragments arranged by size: 2160 2048 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2654 2654 Size: 7210 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2287 2954 3371 3473 3697 3770 3905 Size: 393 655 667 417 102 224 73 135 Cuts at: 3905 4168 5273 5870 6657 6925 1239 Size: 263 1105 597 787 268 1524 Fragments arranged by size: 1524 1105 787 667 655 597 417 393 268 263 224 135 102 73 EcoO109I rG'GnC_Cy Cuts at: 2914 2915 3346 2914 Size: 1 431 6778 Fragments arranged by size: 6778 431 1 EcoRI G'AATT_C Cuts at: 4041 4519 4897 4041 Size: 478 378 6354 Fragments arranged by size: 6354 478 378 EcoRV GAT'ATC Cuts at: 3010 3010 Size: 7210 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2317 2606 2828 4330 2317 Size: 289 222 1502 5197 Fragments arranged by size: 5197 1502 289 222 FspI TGC'GCA Cuts at: 2836 5716 2836 Size: 2880 4330 HaeII r_GCGC'y Cuts at: 1678 3179 6357 1678 Size: 1501 3178 2531 Fragments arranged by size: 3178 2531 1501 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2453 2485 2930 2962 3517 Size: 32 135 32 881 32 445 32 555 Cuts at: 3517 3549 3653 3685 4451 4469 4483 4501 5851 Size: 32 104 32 766 18 14 18 1350 Cuts at: 5851 5883 5925 5957 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1350 881 766 555 445 135 104 42 32 32 32 32 32 32 32 32 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 3436 4554 4800 669 Size: 988 281 1498 1118 246 3079 Fragments arranged by size: 3079 1498 1118 988 281 246 HindIII A'AGCT_T Cuts at: 748 2282 4297 748 Size: 1534 2015 3661 Fragments arranged by size: 3661 2015 1534 HpaI GTT'AAC Cuts at: 4800 4800 Size: 7210 KpnI G_GTAC'C Cuts at: 4535 4535 Size: 7210 MfeI C'AATT_G Cuts at: 4809 4809 Size: 7210 MluI A'CGCG_T Cuts at: 7086 7086 Size: 7210 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2321 3453 3637 3684 4140 6324 6508 1305 Size: 1016 1132 184 47 456 2184 184 2007 Fragments arranged by size: 2184 2007 1132 1016 456 184 184 47 MscI TGG'CCA Cuts at: 10 64 1489 1832 2291 10 Size: 54 1425 343 459 4929 Fragments arranged by size: 4929 1425 459 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4109 4427 4496 5168 5527 5686 518 Size: 648 2943 318 69 672 359 159 2042 Fragments arranged by size: 2943 2042 672 648 359 318 159 69 MspA1I CmG'CkG Cuts at: 1606 2767 3151 3412 3780 3867 4626 5303 6451 Size: 1161 384 261 368 87 759 677 1148 Cuts at: 6451 6696 1606 Size: 245 2120 Fragments arranged by size: 2120 1161 1148 759 677 384 368 261 245 87 NaeI GCC'GGC Cuts at: 4397 4397 Size: 7210 NcoI C'CATG_G Cuts at: 513 1065 4239 513 Size: 552 3174 3484 Fragments arranged by size: 3484 3174 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7210 NgoMIV G'CCGG_C Cuts at: 4395 4395 Size: 7210 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7210 NotI GC'GGCC_GC Cuts at: 4657 4657 Size: 7210 NsiI A_TGCA'T Cuts at: 2215 2215 Size: 7210 NspI r_CATG'y Cuts at: 1132 1229 2755 2888 4568 6113 1132 Size: 97 1526 133 1680 1545 2229 Fragments arranged by size: 2229 1680 1545 1526 133 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7210 PciI A'CATG_T Cuts at: 6109 6109 Size: 7210 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7210 PfoI T'CCnGG_A Cuts at: 3051 3051 Size: 7210 PmeI GTTT'AAAC Cuts at: 4514 4514 Size: 7210 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2233 2265 3791 3823 5279 5311 6792 6824 2233 Size: 32 1526 32 1456 32 1481 32 2619 Fragments arranged by size: 2619 1526 1481 1456 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7210 PsiI TTA'TAA Cuts at: 4780 4780 Size: 7210 PspOMI G'GGCC_C Cuts at: 2914 2914 Size: 7210 PstI C_TGCA'G Cuts at: 830 3132 3525 4360 4562 830 Size: 2302 393 835 202 3478 Fragments arranged by size: 3478 2302 835 393 202 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7210 PvuII CAG'CTG Cuts at: 3151 3412 3151 Size: 261 6949 SacI G_AGCT'C Cuts at: 2656 2656 Size: 7210 SacII CC_GC'GG Cuts at: 2768 2768 Size: 7210 SalI G'TCGA_C Cuts at: 1936 4552 1936 Size: 2616 4594 SapI GCTCTTCn'nnn_ Cuts at: 2577 4406 2577 Size: 1829 5381 SbfI CC_TGCA'GG Cuts at: 3132 4562 3132 Size: 1430 5780 ScaI AGT'ACT Cuts at: 5458 7170 5458 Size: 1712 5498 SfcI C'TryA_G Cuts at: 826 1045 3128 3514 3521 4356 4558 5693 6374 Size: 219 2083 386 7 835 202 1135 681 Cuts at: 6374 6565 826 Size: 191 1471 Fragments arranged by size: 2083 1471 1135 835 681 386 219 202 191 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7210 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7210 SmaI CCC'GGG Cuts at: 1872 3902 4537 1872 Size: 2030 635 4545 Fragments arranged by size: 4545 2030 635 SmlI C'TyrA_G Cuts at: 820 1017 1943 5314 6215 6477 820 Size: 197 926 3371 901 262 1553 Fragments arranged by size: 3371 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5001 493 Size: 4508 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7210 SphI G_CATG'C Cuts at: 1132 2888 4568 1132 Size: 1756 1680 3774 Fragments arranged by size: 3774 1756 1680 SspI AAT'ATT Cuts at: 5 52 3397 5134 5 Size: 47 3345 1737 2081 Fragments arranged by size: 3345 2081 1737 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7210 StyI C'CwwG_G Cuts at: 513 1065 4239 4332 4470 4649 513 Size: 552 3174 93 138 179 3074 Fragments arranged by size: 3174 3074 552 179 138 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2189 5237 5254 5407 5592 6037 325 Size: 1255 609 3048 17 153 185 445 1498 Fragments arranged by size: 3048 1498 1255 609 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2817 3264 4122 Size: 274 80 33 51 1443 840 447 858 Cuts at: 4122 5456 7168 96 Size: 1334 1712 138 Fragments arranged by size: 1712 1443 1334 858 840 447 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2482 2514 3955 3987 7170 7202 2482 Size: 32 1441 32 3183 32 2490 Fragments arranged by size: 3183 2490 1441 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7210 XbaI T'CTAG_A Cuts at: 4546 4546 Size: 7210 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7210 XmaI C'CCGG_G Cuts at: 1870 3900 4535 1870 Size: 2030 635 4545 Fragments arranged by size: 4545 2030 635 XmnI GAAnn'nnTTC Cuts at: 2245 4310 4313 5339 2245 Size: 2065 3 1026 4116 Fragments arranged by size: 4116 2065 1026 3 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3433 4480 5018 276 Size: 53 83 186 936 1899 1047 538 2468 Fragments arranged by size: 2468 1899 1047 936 538 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I Bsu36I EcoNI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI