Restriction map for pFN21AA0082
 (Linear) (Six-base) MAPSORT of: KIAA0082.seq  Check: 2974  from: 1  to: 2505



KIAA0082.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     450    2505 
   Size:        450    2055
 
AatII G_ACGT'C

Cuts at:      0    1430    2477    2505 
   Size:       1430    1047      28

  Fragments arranged by size: 

                1430    1047      28
 
AccI GT'mk_AC

Cuts at:      0    1250    2505 
   Size:       1250    1255
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282    1366    1692    1900    2505 
   Size:        282    1084     326     208     605

  Fragments arranged by size: 

                1084     605     326     282     208
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2460    2505 
   Size:       2460      45
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1162    2440    2505 
   Size:       1162    1278      65

  Fragments arranged by size: 

                1278    1162      65
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1405    1437    2505 
   Size:       1405      32    1068

  Fragments arranged by size: 

                1405    1068      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     414    2505 
   Size:        414    2091
 
ApaI G_GGCC'C

Cuts at:      0     913    2505 
   Size:        913    1592
 
ApoI r'AATT_y

Cuts at:      0     491    1038    1471    2036    2053    2505 
   Size:        491     547     433     565      17     452

  Fragments arranged by size: 

                 565     547     491     452     433      17
 
AvaI C'yCGr_G

Cuts at:      0     656    1895    2505 
   Size:        656    1239     610

  Fragments arranged by size: 

                1239     656     610
 
BamHI G'GATC_C

Cuts at:      0    1619    2505 
   Size:       1619     886
 
BanI G'GyrC_C

Cuts at:      0    2002    2185    2505 
   Size:       2002     183     320

  Fragments arranged by size: 

                2002     320     183
 
BanII G_rGCy'C

Cuts at:      0     446     651     913    2179    2270    2505 
   Size:        446     205     262    1266      91     235

  Fragments arranged by size: 

                1266     446     262     235     205      91
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     681    1888    2505 
   Size:        681    1207     617

  Fragments arranged by size: 

                1207     681     617
 
BbvCI CC'TCA_GC

Cuts at:      0      79    1135    2505 
   Size:         79    1056    1370

  Fragments arranged by size: 

                1370    1056      79
 
BclI T'GATC_A

Cuts at:      0    1454    2505 
   Size:       1454    1051
 
BfrBI ATG'CAT

Cuts at:      0     208    2505 
   Size:        208    2297
 
BglI GCCn_nnn'nGGC

Cuts at:      0     842    2082    2505 
   Size:        842    1240     423

  Fragments arranged by size: 

                1240     842     423
 
BglII A'GATC_T

Cuts at:      0    1829    2505 
   Size:       1829     676
 
BlpI GC'TnA_GC

Cuts at:      0     803    2197    2505 
   Size:        803    1394     308

  Fragments arranged by size: 

                1394     803     308
 
Bme1580I G_kGCm'C

Cuts at:      0     913    1693    2505 
   Size:        913     780     812

  Fragments arranged by size: 

                 913     812     780
 
BmgBI CAC'GTC

Cuts at:      0      89    2505 
   Size:         89    2416
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     440    2505 
   Size:        440    2065
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1041    1300    1592    2505 
   Size:       1041     259     292     913

  Fragments arranged by size: 

                1041     913     292     259
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     949    1468    1765    2163    2505 
   Size:        949     519     297     398     342

  Fragments arranged by size: 

                 949     519     398     342     297
 
Bpu10I CC'TnA_GC

Cuts at:      0      73      79    1135    2505 
   Size:         73       6    1056    1370

  Fragments arranged by size: 

                1370    1056      73       6
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     160    1457    2505 
   Size:        160    1297    1048

  Fragments arranged by size: 

                1297    1048     160
 
BsaBI GATnn'nnATC

Cuts at:      0    1702    2164    2505 
   Size:       1702     462     341

  Fragments arranged by size: 

                1702     462     341
 
BsaHI Gr'CG_yC

Cuts at:      0    1427    2474    2505 
   Size:       1427    1047      31

  Fragments arranged by size: 

                1427    1047      31
 
BsaWI w'CCGG_w

Cuts at:      0    1184    2505 
   Size:       1184    1321
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2464    2494    2505 
   Size:       2464      30      11

  Fragments arranged by size: 

                2464      30      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      21      99     698    2505 
   Size:         21      78     599    1807

  Fragments arranged by size: 

                1807     599      78      21
 
BsiHKAI G_wGCw'C

Cuts at:      0     651    2505 
   Size:        651    1854
 
BsmI GAATG_Cn'

Cuts at:      0      28     208    2417    2505 
   Size:         28     180    2209      88

  Fragments arranged by size: 

                2209     180      88      28
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     273    2505 
   Size:        273    2232
 
Bsp1286I G_dGCh'C

Cuts at:      0     446     651     913    1693    2179    2270    2505 
   Size:        446     205     262     780     486      91     235

  Fragments arranged by size: 

                 780     486     446     262     235     205      91
 
BspEI T'CCGG_A

Cuts at:      0    1184    2505 
   Size:       1184    1321
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     450    1130    1262    2505 
   Size:        450     680     132    1243

  Fragments arranged by size: 

                1243     680     450     132
 
BsrDI GCAATG_nn'

Cuts at:      0     716    2360    2505 
   Size:        716    1644     145

  Fragments arranged by size: 

                1644     716     145
 
BsrFI r'CCGG_y

Cuts at:      0    2219    2390    2505 
   Size:       2219     171     115

  Fragments arranged by size: 

                2219     171     115
 
BsrGI T'GTAC_A

Cuts at:      0    2117    2505 
   Size:       2117     388
 
BssSI C'ACGA_G

Cuts at:      0    1634    2505 
   Size:       1634     871
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      67    2505 
   Size:         67    2438
 
BstBI TT'CG_AA

Cuts at:      0     962    2505 
   Size:        962    1543
 
BstYI r'GATC_y

Cuts at:      0    1130    1619    1751    1829    2505 
   Size:       1130     489     132      78     676

  Fragments arranged by size: 

                1130     676     489     132      78
 
BtgI C'CryG_G

Cuts at:      0     760    2234    2505 
   Size:        760    1474     271

  Fragments arranged by size: 

                1474     760     271
 
BtsI GCAGTG_nn'

Cuts at:      0      26     607    1391    1723    1797    2505 
   Size:         26     581     784     332      74     708

  Fragments arranged by size: 

                 784     708     581     332      74      26
 
DraIII CAC_nnn'GTG

Cuts at:      0     594    1570    2505 
   Size:        594     976     935

  Fragments arranged by size: 

                 976     935     594
 
EaeI y'GGCC_r

Cuts at:      0     284    1845    2388    2505 
   Size:        284    1561     543     117

  Fragments arranged by size: 

                1561     543     284     117
 
EarI CTCTTCn'nnn_

Cuts at:      0     369     572     629     964    1291    1384    2123    2401 
   Size:        369     203      57     335     327      93     739     278

Cuts at:   2401    2505 
   Size:        104

  Fragments arranged by size: 

                 739     369     335     327     278     203     104      93
                  57
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1744    2505 
   Size:       1744     761
 
EcoICRI GAG'CTC

Cuts at:      0     649    2505 
   Size:        649    1856
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     282     949    1366    1468    1692    1765    1900    2163 
   Size:        282     667     417     102     224      73     135     263

Cuts at:   2163    2505 
   Size:        342

  Fragments arranged by size: 

                 667     417     342     282     263     224     135     102
                  73
 
EcoO109I rG'GnC_Cy

Cuts at:      0     909     910    1341    2505 
   Size:        909       1     431    1164

  Fragments arranged by size: 

                1164     909     431       1
 
EcoRI G'AATT_C

Cuts at:      0    2036    2505 
   Size:       2036     469
 
EcoRV GAT'ATC

Cuts at:      0    1005    2505 
   Size:       1005    1500
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     312     601     823    2325    2505 
   Size:        312     289     222    1502     180

  Fragments arranged by size: 

                1502     312     289     222     180
 
FspI TGC'GCA

Cuts at:      0     831    2505 
   Size:        831    1674
 
HaeII r_GCGC'y

Cuts at:      0    1174    2505 
   Size:       1174    1331
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     448     480     925     957    1512    1544    1648    1680 
   Size:        448      32     445      32     555      32     104      32

Cuts at:   1680    2446    2464    2478    2496    2505 
   Size:        766      18      14      18       9

  Fragments arranged by size: 

                 766     555     448     445     104      32      32      32
                  32      18      18      14       9
 
HincII GTy'rAC

Cuts at:      0    1431    2505 
   Size:       1431    1074
 
HindIII A'AGCT_T

Cuts at:      0     277    2292    2505 
   Size:        277    2015     213

  Fragments arranged by size: 

                2015     277     213
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     316    1448    1632    1679    2135    2505 
   Size:        316    1132     184      47     456     370

  Fragments arranged by size: 

                1132     456     370     316     184      47
 
MscI TGG'CCA

Cuts at:      0     286    2505 
   Size:        286    2219
 
MslI CAynn'nnrTG

Cuts at:      0    2104    2422    2491    2505 
   Size:       2104     318      69      14

  Fragments arranged by size: 

                2104     318      69      14
 
MspA1I CmG'CkG

Cuts at:      0     762    1146    1407    1775    1862    2505 
   Size:        762     384     261     368      87     643

  Fragments arranged by size: 

                 762     643     384     368     261      87
 
NaeI GCC'GGC

Cuts at:      0    2392    2505 
   Size:       2392     113
 
NcoI C'CATG_G

Cuts at:      0    2234    2505 
   Size:       2234     271
 
NgoMIV G'CCGG_C

Cuts at:      0    2390    2505 
   Size:       2390     115
 
NsiI A_TGCA'T

Cuts at:      0     210    2505 
   Size:        210    2295
 
NspI r_CATG'y

Cuts at:      0     750     883    2505 
   Size:        750     133    1622

  Fragments arranged by size: 

                1622     750     133
 
PfoI T'CCnGG_A

Cuts at:      0    1046    2505 
   Size:       1046    1459
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     228     260    1786    1818    2505 
   Size:        228      32    1526      32     687

  Fragments arranged by size: 

                1526     687     228      32      32
 
PspOMI G'GGCC_C

Cuts at:      0     909    2505 
   Size:        909    1596
 
PstI C_TGCA'G

Cuts at:      0    1127    1520    2355    2505 
   Size:       1127     393     835     150

  Fragments arranged by size: 

                1127     835     393     150
 
PvuII CAG'CTG

Cuts at:      0    1146    1407    2505 
   Size:       1146     261    1098

  Fragments arranged by size: 

                1146    1098     261
 
SacI G_AGCT'C

Cuts at:      0     651    2505 
   Size:        651    1854
 
SacII CC_GC'GG

Cuts at:      0     763    2505 
   Size:        763    1742
 
SapI GCTCTTCn'nnn_

Cuts at:      0     572    2401    2505 
   Size:        572    1829     104

  Fragments arranged by size: 

                1829     572     104
 
SbfI CC_TGCA'GG

Cuts at:      0    1127    2505 
   Size:       1127    1378
 
SfcI C'TryA_G

Cuts at:      0    1123    1509    1516    2351    2505 
   Size:       1123     386       7     835     154

  Fragments arranged by size: 

                1123     835     386     154       7
 
SmaI CCC'GGG

Cuts at:      0    1897    2505 
   Size:       1897     608
 
SphI G_CATG'C

Cuts at:      0     883    2505 
   Size:        883    1622
 
SspI AAT'ATT

Cuts at:      0    1392    2505 
   Size:       1392    1113
 
StyI C'CwwG_G

Cuts at:      0    2234    2327    2465    2505 
   Size:       2234      93     138      40

  Fragments arranged by size: 

                2234     138      93      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     184    2505 
   Size:        184    2321
 
TatI w'GTAC_w

Cuts at:      0     812    1259    2117    2505 
   Size:        812     447     858     388

  Fragments arranged by size: 

                 858     812     447     388
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     477     509    1950    1982    2505 
   Size:        477      32    1441      32     523

  Fragments arranged by size: 

                1441     523     477      32      32
 
XmaI C'CCGG_G

Cuts at:      0    1895    2505 
   Size:       1895     610
 
XmnI GAAnn'nnTTC

Cuts at:      0     240    2305    2308    2505 
   Size:        240    2065       3     197

  Fragments arranged by size: 

                2065     240     197       3
 
ZraI GAC'GTC

Cuts at:      0    1428    2475    2505 
   Size:       1428    1047      30

  Fragments arranged by size: 

                1428    1047      30
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AhdI     AlfI     AloI    AlwNI
     ApaI     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI     BtgI     BtsI
   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NsiI     NspI
     PfoI     PpiI   PspOMI     PstI    PvuII     SacI    SacII     SapI
     SbfI     SfcI     SmaI     SphI     SspI     StyI    TaqII     TatI
     TstI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AleI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI    BciVI
     BmtI    BpuEI    BsaAI    BseYI     BsgI    BsiEI    BsiWI    BspHI
    BsrBI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI     DrdI     EagI    EcoNI     FseI    FspAI     HpaI
     KasI     KpnI     MfeI     MluI     NarI     NdeI     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PmeI     PmlI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SrfI     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI
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