Restriction map for
pFN21AA0073
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I AocI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BanII BbrPI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaI BseAI
BsiEI BsiHKAI BsiWI BsmI BsoBI
BspDI BspEI BspMI BsrBI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FseI FspI
HgaI HincII HindII HpaI KpnI
KspI MaeI MluI MroI MspA1I
NaeI NcoI NgoMIV NheI NotI
NruI NspV PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccII 1746 CGCG CG^CG
AcyI 44 GGCGCC G[GA]^CG[CT]C
AflIII 333 ACATGT A^C[GA][CT]GT
AgeI 1157 ACCGGT A^CCGGT
Aor51HI 143 AGCGCT AGC^GCT
BalI 179 TGGCCA TGG^CCA
BamHI 1765 GGATCC G^GATCC
BanI 44 GGCGCC G^G[CT][GA]CC
BbeI 44 GGCGCC GGCGC^C
BbsI 717 GTCTTC GAAGAC(2/6)
BclI 500 TGATCA T^GATCA
BmyI 1533 GTGCCC G[AGT]GC[ACT]^C
BpmI 1631 CTGGAG CTGGAG(16/14)
BpuAI 717 GTCTTC GAAGAC(2/6)
BsaBI 1387 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 44 GGCGCC G[GA]^CG[CT]C
BsgI 1382 GTGCAG GTGCAG(16/14)
BsmBI 33 GAGACG CGTCTC(1/5)
Bsp1286I 1533 GTGCCC G[AGT]GC[ACT]^C
BspLU11I 333 ACATGT A^CATGT
BsrDI 466 GCAATG GCAATG(2/0)
BsrFI 1157 ACCGGT [GA]^CCGG[CT]
BstUI 1746 CGCG CG^CG
BstXI 1792 CCAACGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 93 GCTGGC GC[ACGT]^[ACGT]GC
Cfr10I 1157 ACCGGT [GA]^CCGG[CT]
DraIII 1102 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 143 AGCGCT AGC^GCT
EcoT14I 1798 CCTTGG C^C[AT][AT]GG
EcoT22I 262 ATGCAT ATGCA^T
FauI 1747 GCGGG CCCGC(4/6)
FbaI 500 TGATCA T^GATCA
Hin1I 44 GGCGCC G[GA]^CG[CT]C
HindIII 1265 AAGCTT A^AGCTT
KasI 44 GGCGCC G^GCGCC
MamI 1387 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1204 CAATTG C^AATTG
MluNI 179 TGGCCA TGG^CCA
MscI 179 TGGCCA TGG^CCA
MunI 1204 CAATTG C^AATTG
MvnI 1746 CGCG CG^CG
NarI 44 GGCGCC GG^CGCC
NdeI 1668 CATATG CA^TATG
NsiI 262 ATGCAT ATGCA^T
PacI 1072 TTAATTAA TTAAT^TAA
PinAI 1157 ACCGGT A^CCGGT
SapI 139 GAAGAGC GCTCTTC(1/4)
SfoI 44 GGCGCC GGC^GCC
SmlI 1445 CTCAAG C^T[CT][GA]AG
StyI 1798 CCTTGG C^C[AT][AT]GG
TaqI 1395 TCGA T^CGA
TthHB8I 1395 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AseI 1071 ATTAAT AT^TAAT
1075 ATTAAT
AsnI 1071 ATTAAT AT^TAAT
1075 ATTAAT
BcnI 48 CCGGG CC^[GC]GG
54 CCCGG
BsaWI 63 ACCGGA [AT]^CCGG[AT]
1157 ACCGGT
BsmFI 50 GGGAC GGGAC(10/14)
114 GTCCC
Bsp1407I 1318 TGTACA T^GTACA
1854 TGTACA
BsrGI 1318 TGTACA T^GTACA
1854 TGTACA
DraII 50 GGGACCC [GA]G^G[ACGT]CC[CT]
1455 AGGACCT
EcoO109I 50 GGGACCC [GA]G^G[ACGT]CC[CT]
1455 AGGACCT
HaeII 44 GGCGCC [GA]GCGC^[CT]
143 AGCGCT
MwoI 172 GCAACACTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1441 GCTACTCAAGC
NciI 48 CCGGG CC^[GC]GG
54 CCCGG
PleI 212 GAGTC GAGTC(4/5)
947 GAGTC
PpuMI 50 GGGACCC [GA]G^G[AT]CC[CT]
1455 AGGACCT
PshBI 1071 ATTAAT AT^TAAT
1075 ATTAAT
SfcI 1034 CTGTAG C^T[GA][CT]AG
1253 CTATAG
SspBI 1318 TGTACA T^GTACA
1854 TGTACA
Tsp45I 392 GTCAC ^GT[GC]AC
1834 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BbvI BsaJI
BseRI BsiYI BslI BsmAI BspHI
BsrI BssKI BstF5I BstNI BstYI
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI NdeII NlaIII
NlaIV NspI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SspI TfiI
Tru9I TseI Tsp509I TspRI XhoII