Restriction map for pFN21AA0073
 (Circular) (Six-base) MAPSORT of: pFN21AA0073.seq  Check: 5654  from: 1  to: 6643



pFN21AA0073  6643 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:22  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4453     278 
   Size:         53      83     186     936    2917    2468

  Fragments arranged by size: 

                2917    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3986    1937 
   Size:       2049    4594
 
Acc65I G'GTAC_C

Cuts at:   3964    3964 
   Size:       6643
 
AclI AA'CG_TT

Cuts at:   1563    4770    5143    1563 
   Size:       3207     373    3063

  Fragments arranged by size: 

                3207    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2562    3275    3477    4706    6090    6358    1632 
   Size:        930     713     202    1229    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1229     930     713     268     202
 
AfeI AGC'GCT

Cuts at:   2150    2150 
   Size:       6643
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6446
 
AflIII A'CryG_T

Cuts at:   1203    2338    5542    6519    1203 
   Size:       1135    3204     977    1327

  Fragments arranged by size: 

                3204    1327    1135     977
 
AgeI A'CCGG_T

Cuts at:   3162    5474    3162 
   Size:       2312    4331
 
AhdI GACnn_n'nnGTC

Cuts at:   5372    5372 
   Size:       6643
 
AleI CACnn'nnGTG

Cuts at:   1166    2288    1166 
   Size:       1122    5521
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2282    3264    1184 
   Size:       1098     982    4563

  Fragments arranged by size: 

                4563    1098     982
 
AlwNI CAG_nnn'CTG

Cuts at:   2585    3395    3653    4386    5958    2585 
   Size:        810     258     733    1572    3270

  Fragments arranged by size: 

                3270    1572     810     733     258
 
ApaLI G'TGCA_C

Cuts at:   4700    5856    4700 
   Size:       1156    5487
 
ApoI r'AATT_y

Cuts at:   1493    2375    2610    2777    2828    2919    3020    3686    3698 
   Size:        882     235     167      51      91     101     666      12

Cuts at:   3698    3952    4178    4330    6439    1493 
   Size:        254     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     882     666     254     235     226     167
                 152     101      91      51      12
 
AseI AT'TA_AT

Cuts at:    160    1032    3077    3081    5197     160 
   Size:        872    2045       4    2116    1606

  Fragments arranged by size: 

                2116    2045    1606     872       4
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6643
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3968    1870 
   Size:         73    2025    4545

  Fragments arranged by size: 

                4545    2025      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   3777    3810    3777 
   Size:         33    6610
 
BamHI G'GATC_C

Cuts at:   3770    3973    4340    3770 
   Size:        203     367    6073

  Fragments arranged by size: 

                6073     367     203
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2049    3964    5419     618 
   Size:        325     217     627     262    1915    1455    1842

  Fragments arranged by size: 

                1915    1842    1455     627     325     262     217
 
BanII G_rGCy'C

Cuts at:   1391    3962    1391 
   Size:       2571    4072
 
BbeI G_GCGC'C

Cuts at:   2053    2053 
   Size:       6643
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2715     928 
   Size:        971     816    4856

  Fragments arranged by size: 

                4856     971     816
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4834    4868    1903 
   Size:         34    2897      34    3678

  Fragments arranged by size: 

                3678    2897      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4535    5745    4535 
   Size:       1210    5433
 
BclI T'GATC_A

Cuts at:   1548    2505    1548 
   Size:        957    5686
 
BfrBI ATG'CAT

Cuts at:   2269    2269 
   Size:       6643
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    5254     136 
   Size:        107     122      71     631     605     337    3245    1525

  Fragments arranged by size: 

                3245    1525     631     605     337     122     107      71
            
 
BglII A'GATC_T

Cuts at:   6638    6638 
   Size:       6643
 
BlpI GC'TnA_GC

Cuts at:   4060    4060 
   Size:       6643
 
Bme1580I G_kGCm'C

Cuts at:   1790    3542    4704    5860    1790 
   Size:       1752    1162    1156    2573

  Fragments arranged by size: 

                2573    1752    1162    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6643
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2232    2696    2766 
   Size:        416      53      83     399      24     815     464      70

Cuts at:   2766    2806    3769    4374    5332     442 
   Size:         40     963     605     958    1753

  Fragments arranged by size: 

                1753     963     958     815     605     464     416     399
                  83      70      53      40      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6643
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3657    5303    1239 
   Size:       2418    1646    2579

  Fragments arranged by size: 

                2579    2418    1646
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3435    4768    5633    5931    3435 
   Size:       1333     865     298    4147

  Fragments arranged by size: 

                4147    1333     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5306     882 
   Size:        617    3807    2219

  Fragments arranged by size: 

                3807    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4434     493 
   Size:        711    3230    2702

  Fragments arranged by size: 

                3230    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   3396    6637    3396 
   Size:       3241    3402
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2050    4450    4832    5467 
   Size:         53      83     186     936     517    2400     382     635

Cuts at:   5467     275 
   Size:       1451

  Fragments arranged by size: 

                2400    1451     936     635     517     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2068    3162    5076    5474    5748    5895    6306    2068 
   Size:       1094    1914     398     274     147     411    2405

  Fragments arranged by size: 

                2405    1914    1094     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2746    2776    1178 
   Size:         30     165      30    1343      30    5045

  Fragments arranged by size: 

                5045    1343     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2150    2646    2754    3678    3696    1186 
   Size:        964     496     108     924      18    4133

  Fragments arranged by size: 

                4133     964     924     496     108      18
 
BseYI C'CCAG_C

Cuts at:   2151    3130    5846    6342    2151 
   Size:        979    2716     496    2452

  Fragments arranged by size: 

                2716    2452     979     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    3408    1163 
   Size:        573    1672    4398

  Fragments arranged by size: 

                4398    1672     573
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    4093    4854    5882    1540 
   Size:        275     186    2092     761    1028    2301

  Fragments arranged by size: 

                2301    2092    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   4704    4789    5860    4704 
   Size:         85    1071    5487

  Fragments arranged by size: 

                5487    1071      85
 
BsmI GAATG_Cn'

Cuts at:   4154    4247    4154 
   Size:         93    6550
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2032    2032 
   Size:       6643
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3542    3962    4704    4789    5860    1391 
   Size:        399    1752     420     742      85    1071    2174

  Fragments arranged by size: 

                2174    1752    1071     742     420     399      85
 
BspEI T'CCGG_A

Cuts at:   6306    6306 
   Size:       6643
 
BspHI T'CATG_A

Cuts at:   2329    2767    2946    4532    6262    2329 
   Size:        438     179    1586    1730    2710

  Fragments arranged by size: 

                2710    1730    1586     438     179
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3998     844 
   Size:       3154    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4530    1394 
   Size:        429    2707    3507

  Fragments arranged by size: 

                3507    2707     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2478    5138    5312      65 
   Size:       1183    1230    2660     174    1396

  Fragments arranged by size: 

                2660    1396    1230    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3162    5287    5474    1700 
   Size:        110    1352    2125     187    2869

  Fragments arranged by size: 

                2869    2125    1352     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    3323    3859      96 
   Size:       3227     536    2880

  Fragments arranged by size: 

                3227    2880     536
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6643
 
BssSI C'ACGA_G

Cuts at:   4703    5715    4703 
   Size:       1012    5631
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6643
 
BstBI TT'CG_AA

Cuts at:   5447    5447 
   Size:       6643
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6643
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3804    3804 
   Size:       6643
 
BstYI r'GATC_y

Cuts at:   1972    2550    3640    3770    3867    3973    4340    4725    4742 
   Size:        578    1090     130      97     106     367     385      17

Cuts at:   4742    6194    6280    6292    6638    1972 
   Size:       1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1452    1090     578     385     367     346     130
                 106      97      86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    5152

  Fragments arranged by size: 

                5152     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5904

  Fragments arranged by size: 

                5904     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2115    4159    4953    4973    2115 
   Size:       2044     794      20    3785

  Fragments arranged by size: 

                3785    2044     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6643
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6608
 
DraI TTT'AAA

Cuts at:   3947    3947 
   Size:       6643
 
DraIII CAC_nnn'GTG

Cuts at:   3112    3112 
   Size:       6643
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5650     809 
   Size:        507    4334    1802

  Fragments arranged by size: 

                4334    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2184    2626    3025 
   Size:         54    1425      50     275      18     354     442     399

Cuts at:   3025    4090    4979    6608       8 
   Size:       1065     889    1629      43

  Fragments arranged by size: 

                1629    1425    1065     889     442     399     354     275
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    4090    1537 
   Size:        275    2278    4090

  Fragments arranged by size: 

                4090    2278     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2139    2186    2358    4573    1353 
   Size:        786      47     172    2215    3423

  Fragments arranged by size: 

                3423    2215     786     172      47
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2027    5230    5604    5750     387 
   Size:       1064     138     438    3203     374     146    1280

  Fragments arranged by size: 

                3203    1280    1064     438     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2562    3275    3477    3657    4706    5303    6090 
   Size:        393     930     713     202     180    1049     597     787

Cuts at:   6090    6358    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1049     930     787     713     597     393     268
                 202     180
 
EcoO109I rG'GnC_Cy

Cuts at:   2056    3461    2056 
   Size:       1405    5238
 
EcoRI G'AATT_C

Cuts at:   3952    4330    3952 
   Size:        378    6265
 
FspI TGC'GCA

Cuts at:   5149    5149 
   Size:       6643
 
HaeII r_GCGC'y

Cuts at:   1678    2053    2152    5790    1678 
   Size:        375      99    3638    2531

  Fragments arranged by size: 

                3638    2531     375      99
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2265    2297    2746    2778    3625 
   Size:         32     135      32     693      32     449      32     847

Cuts at:   3625    3657    5284    5316    5358    5390    1373 
   Size:         32    1627      32      42      32    2626

  Fragments arranged by size: 

                2626    1627     847     693     449     135      42      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3987    4233     669 
   Size:        988     281    2049     246    3079

  Fragments arranged by size: 

                3079    2049     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3270     748 
   Size:       2522    4121
 
HpaI GTT'AAC

Cuts at:   4233    4233 
   Size:       6643
 
KasI G'GCGC_C

Cuts at:   2049    2049 
   Size:       6643
 
KpnI G_GTAC'C

Cuts at:   3968    3968 
   Size:       6643
 
MfeI C'AATT_G

Cuts at:   3209    4242    3209 
   Size:       1033    5610
 
MluI A'CGCG_T

Cuts at:   6519    6519 
   Size:       6643
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2137    2876    3033    3668    3897    5757    5941    1305 
   Size:        832     739     157     635     229    1860     184    2007

  Fragments arranged by size: 

                2007    1860     832     739     635     229     184     157
            
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2186      10 
   Size:         54    1425     343     354    4467

  Fragments arranged by size: 

                4467    1425     354     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2274    2288    3500    4601    4960    5119     518 
   Size:        648    1108      14    1212    1101     359     159    2042

  Fragments arranged by size: 

                2042    1212    1108    1101     648     359     159      14
            
 
MspA1I CmG'CkG

Cuts at:   1606    4059    4736    5884    6129    1606 
   Size:       2453     677    1148     245    2120

  Fragments arranged by size: 

                2453    2120    1148     677     245
 
NarI GG'CG_CC

Cuts at:   2050    2050 
   Size:       6643
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    5152

  Fragments arranged by size: 

                5152     939     552
 
NdeI CA'TA_TG

Cuts at:    387    3674     387 
   Size:       3287    3356
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6643
 
NotI GC'GGCC_GC

Cuts at:   4090    4090 
   Size:       6643
 
NsiI A_TGCA'T

Cuts at:   2271    2271 
   Size:       6643
 
NspI r_CATG'y

Cuts at:   1132    1229    2269    2342    2515    2977    4001    5546    1132 
   Size:         97    1040      73     173     462    1024    1545    2229

  Fragments arranged by size: 

                2229    1545    1040    1024     462     173      97      73
            
 
PacI TTA_AT'TAA

Cuts at:   3081    3081 
   Size:       6643
 
PasI CC'CwG_GG

Cuts at:   1348    2561    1348 
   Size:       1213    5430
 
PciI A'CATG_T

Cuts at:   2338    5542    2338 
   Size:       3204    3439
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6643
 
PfoI T'CCnGG_A

Cuts at:   2533    2533 
   Size:       6643
 
PmeI GTTT'AAAC

Cuts at:   3947    3947 
   Size:       6643
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4712    4744    6225    6257    4712 
   Size:         32    1481      32    5098

  Fragments arranged by size: 

                5098    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2056    3461    2056 
   Size:       1405    5238
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6643
 
PsiI TTA'TAA

Cuts at:   3146    3588    4213    3146 
   Size:        442     625    5576

  Fragments arranged by size: 

                5576     625     442
 
PstI C_TGCA'G

Cuts at:    830    3995     830 
   Size:       3165    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6643
 
SalI G'TCGA_C

Cuts at:   1936    3985    1936 
   Size:       2049    4594
 
SanDI GG'GwC_CC

Cuts at:   2056    2056 
   Size:       6643
 
SapI GCTCTTCn'nnn_

Cuts at:   2139    2139 
   Size:       6643
 
SbfI CC_TGCA'GG

Cuts at:   3995    3995 
   Size:       6643
 
ScaI AGT'ACT

Cuts at:   4891    6603    4891 
   Size:       1712    4931
 
SfcI C'TryA_G

Cuts at:    826    1045    3039    3258    3991    5126    5807    5998     826 
   Size:        219    1994     219     733    1135     681     191    1471

  Fragments arranged by size: 

                1994    1471    1135     733     681     219     219     191
            
 
SfoI GGC'GCC

Cuts at:   2051    2051 
   Size:       6643
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6643
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6643
 
SmaI CCC'GGG

Cuts at:   1872    3970    1872 
   Size:       2098    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3450    4747    5648    5910     820 
   Size:        197     926    1507    1297     901     262    1553

  Fragments arranged by size: 

                1553    1507    1297     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4434     493 
   Size:       3941    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6643
 
SphI G_CATG'C

Cuts at:   1132    4001    1132 
   Size:       2869    3774
 
SspI AAT'ATT

Cuts at:      5      52    3196    3298    3931    4567       5 
   Size:         47    3144     102     633     636    2081

  Fragments arranged by size: 

                3144    2081     636     633     102      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6643
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3803    4082     513 
   Size:        552     939    1799     279    3074

  Fragments arranged by size: 

                3074    1799     939     552     279
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2300    2447    4670    4687    4840    5025    5470 
   Size:       1255     720     147    2223      17     153     185     445

Cuts at:   5470     325 
   Size:       1498

  Fragments arranged by size: 

                2223    1498    1255     720     445     185     153     147
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2600    3323    3859 
   Size:        274      80      33      51    1443     623     723     536

Cuts at:   3859    4889    6601      96 
   Size:       1030    1712     138

  Fragments arranged by size: 

                1712    1443    1030     723     623     536     274     138
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3096    3128    6603    6635    3096 
   Size:         32    3475      32    3104

  Fragments arranged by size: 

                3475    3104      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6643
 
XbaI T'CTAG_A

Cuts at:   3979    3979 
   Size:       6643
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6643
 
XmaI C'CCGG_G

Cuts at:   1870    3968    1870 
   Size:       2098    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4772    4772 
   Size:       6643
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4451     276 
   Size:         53      83     186     936    2917    2468

  Fragments arranged by size: 

                2917    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NarI     NcoI
     NdeI     NheI     NotI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI
     PvuI     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII    BbvCI     BplI   Bpu10I
    BsiWI  BstZ17I   Bsu36I  EcoICRI    EcoNI    EcoRV     FalI     FseI
    FspAI     NaeI   NgoMIV     NruI     PmlI   PspOMI    PspXI     PsrI
    PvuII    RsrII     SacI    SacII    SexAI     SfiI     SrfI     SwaI
     XcmI
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