(Circular) (Six-base) MAPSORT of: pFN21AA0073.seq Check: 5654 from: 1 to: 6643 pFN21AA0073 6643 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4453 278 Size: 53 83 186 936 2917 2468 Fragments arranged by size: 2917 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3986 1937 Size: 2049 4594 Acc65I G'GTAC_C Cuts at: 3964 3964 Size: 6643 AclI AA'CG_TT Cuts at: 1563 4770 5143 1563 Size: 3207 373 3063 Fragments arranged by size: 3207 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2562 3275 3477 4706 6090 6358 1632 Size: 930 713 202 1229 1384 268 1917 Fragments arranged by size: 1917 1384 1229 930 713 268 202 AfeI AGC'GCT Cuts at: 2150 2150 Size: 6643 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6446 AflIII A'CryG_T Cuts at: 1203 2338 5542 6519 1203 Size: 1135 3204 977 1327 Fragments arranged by size: 3204 1327 1135 977 AgeI A'CCGG_T Cuts at: 3162 5474 3162 Size: 2312 4331 AhdI GACnn_n'nnGTC Cuts at: 5372 5372 Size: 6643 AleI CACnn'nnGTG Cuts at: 1166 2288 1166 Size: 1122 5521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2282 3264 1184 Size: 1098 982 4563 Fragments arranged by size: 4563 1098 982 AlwNI CAG_nnn'CTG Cuts at: 2585 3395 3653 4386 5958 2585 Size: 810 258 733 1572 3270 Fragments arranged by size: 3270 1572 810 733 258 ApaLI G'TGCA_C Cuts at: 4700 5856 4700 Size: 1156 5487 ApoI r'AATT_y Cuts at: 1493 2375 2610 2777 2828 2919 3020 3686 3698 Size: 882 235 167 51 91 101 666 12 Cuts at: 3698 3952 4178 4330 6439 1493 Size: 254 226 152 2109 1697 Fragments arranged by size: 2109 1697 882 666 254 235 226 167 152 101 91 51 12 AseI AT'TA_AT Cuts at: 160 1032 3077 3081 5197 160 Size: 872 2045 4 2116 1606 Fragments arranged by size: 2116 2045 1606 872 4 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6643 AvaI C'yCGr_G Cuts at: 1870 1943 3968 1870 Size: 73 2025 4545 Fragments arranged by size: 4545 2025 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3777 3810 3777 Size: 33 6610 BamHI G'GATC_C Cuts at: 3770 3973 4340 3770 Size: 203 367 6073 Fragments arranged by size: 6073 367 203 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2049 3964 5419 618 Size: 325 217 627 262 1915 1455 1842 Fragments arranged by size: 1915 1842 1455 627 325 262 217 BanII G_rGCy'C Cuts at: 1391 3962 1391 Size: 2571 4072 BbeI G_GCGC'C Cuts at: 2053 2053 Size: 6643 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2715 928 Size: 971 816 4856 Fragments arranged by size: 4856 971 816 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4834 4868 1903 Size: 34 2897 34 3678 Fragments arranged by size: 3678 2897 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4535 5745 4535 Size: 1210 5433 BclI T'GATC_A Cuts at: 1548 2505 1548 Size: 957 5686 BfrBI ATG'CAT Cuts at: 2269 2269 Size: 6643 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5254 136 Size: 107 122 71 631 605 337 3245 1525 Fragments arranged by size: 3245 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6638 6638 Size: 6643 BlpI GC'TnA_GC Cuts at: 4060 4060 Size: 6643 Bme1580I G_kGCm'C Cuts at: 1790 3542 4704 5860 1790 Size: 1752 1162 1156 2573 Fragments arranged by size: 2573 1752 1162 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6643 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2232 2696 2766 Size: 416 53 83 399 24 815 464 70 Cuts at: 2766 2806 3769 4374 5332 442 Size: 40 963 605 958 1753 Fragments arranged by size: 1753 963 958 815 605 464 416 399 83 70 53 40 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6643 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3657 5303 1239 Size: 2418 1646 2579 Fragments arranged by size: 2579 2418 1646 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3435 4768 5633 5931 3435 Size: 1333 865 298 4147 Fragments arranged by size: 4147 1333 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5306 882 Size: 617 3807 2219 Fragments arranged by size: 3807 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4434 493 Size: 711 3230 2702 Fragments arranged by size: 3230 2702 711 BsaBI GATnn'nnATC Cuts at: 3396 6637 3396 Size: 3241 3402 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2050 4450 4832 5467 Size: 53 83 186 936 517 2400 382 635 Cuts at: 5467 275 Size: 1451 Fragments arranged by size: 2400 1451 936 635 517 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2068 3162 5076 5474 5748 5895 6306 2068 Size: 1094 1914 398 274 147 411 2405 Fragments arranged by size: 2405 1914 1094 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2746 2776 1178 Size: 30 165 30 1343 30 5045 Fragments arranged by size: 5045 1343 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2150 2646 2754 3678 3696 1186 Size: 964 496 108 924 18 4133 Fragments arranged by size: 4133 964 924 496 108 18 BseYI C'CCAG_C Cuts at: 2151 3130 5846 6342 2151 Size: 979 2716 496 2452 Fragments arranged by size: 2716 2452 979 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3408 1163 Size: 573 1672 4398 Fragments arranged by size: 4398 1672 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4093 4854 5882 1540 Size: 275 186 2092 761 1028 2301 Fragments arranged by size: 2301 2092 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4704 4789 5860 4704 Size: 85 1071 5487 Fragments arranged by size: 5487 1071 85 BsmI GAATG_Cn' Cuts at: 4154 4247 4154 Size: 93 6550 BsmBI CGTCTCn'nnnn_ Cuts at: 2032 2032 Size: 6643 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3542 3962 4704 4789 5860 1391 Size: 399 1752 420 742 85 1071 2174 Fragments arranged by size: 2174 1752 1071 742 420 399 85 BspEI T'CCGG_A Cuts at: 6306 6306 Size: 6643 BspHI T'CATG_A Cuts at: 2329 2767 2946 4532 6262 2329 Size: 438 179 1586 1730 2710 Fragments arranged by size: 2710 1730 1586 438 179 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3998 844 Size: 3154 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4530 1394 Size: 429 2707 3507 Fragments arranged by size: 3507 2707 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2478 5138 5312 65 Size: 1183 1230 2660 174 1396 Fragments arranged by size: 2660 1396 1230 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3162 5287 5474 1700 Size: 110 1352 2125 187 2869 Fragments arranged by size: 2869 2125 1352 187 110 BsrGI T'GTAC_A Cuts at: 96 3323 3859 96 Size: 3227 536 2880 Fragments arranged by size: 3227 2880 536 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6643 BssSI C'ACGA_G Cuts at: 4703 5715 4703 Size: 1012 5631 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6643 BstBI TT'CG_AA Cuts at: 5447 5447 Size: 6643 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6643 BstXI CCAn_nnnn'nTGG Cuts at: 3804 3804 Size: 6643 BstYI r'GATC_y Cuts at: 1972 2550 3640 3770 3867 3973 4340 4725 4742 Size: 578 1090 130 97 106 367 385 17 Cuts at: 4742 6194 6280 6292 6638 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1090 578 385 367 346 130 106 97 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5152 Fragments arranged by size: 5152 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5904 Fragments arranged by size: 5904 683 56 BtsI GCAGTG_nn' Cuts at: 2115 4159 4953 4973 2115 Size: 2044 794 20 3785 Fragments arranged by size: 3785 2044 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6643 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6608 DraI TTT'AAA Cuts at: 3947 3947 Size: 6643 DraIII CAC_nnn'GTG Cuts at: 3112 3112 Size: 6643 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5650 809 Size: 507 4334 1802 Fragments arranged by size: 4334 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 2626 3025 Size: 54 1425 50 275 18 354 442 399 Cuts at: 3025 4090 4979 6608 8 Size: 1065 889 1629 43 Fragments arranged by size: 1629 1425 1065 889 442 399 354 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4090 1537 Size: 275 2278 4090 Fragments arranged by size: 4090 2278 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2139 2186 2358 4573 1353 Size: 786 47 172 2215 3423 Fragments arranged by size: 3423 2215 786 172 47 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 5230 5604 5750 387 Size: 1064 138 438 3203 374 146 1280 Fragments arranged by size: 3203 1280 1064 438 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2562 3275 3477 3657 4706 5303 6090 Size: 393 930 713 202 180 1049 597 787 Cuts at: 6090 6358 1239 Size: 268 1524 Fragments arranged by size: 1524 1049 930 787 713 597 393 268 202 180 EcoO109I rG'GnC_Cy Cuts at: 2056 3461 2056 Size: 1405 5238 EcoRI G'AATT_C Cuts at: 3952 4330 3952 Size: 378 6265 FspI TGC'GCA Cuts at: 5149 5149 Size: 6643 HaeII r_GCGC'y Cuts at: 1678 2053 2152 5790 1678 Size: 375 99 3638 2531 Fragments arranged by size: 3638 2531 375 99 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2265 2297 2746 2778 3625 Size: 32 135 32 693 32 449 32 847 Cuts at: 3625 3657 5284 5316 5358 5390 1373 Size: 32 1627 32 42 32 2626 Fragments arranged by size: 2626 1627 847 693 449 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3987 4233 669 Size: 988 281 2049 246 3079 Fragments arranged by size: 3079 2049 988 281 246 HindIII A'AGCT_T Cuts at: 748 3270 748 Size: 2522 4121 HpaI GTT'AAC Cuts at: 4233 4233 Size: 6643 KasI G'GCGC_C Cuts at: 2049 2049 Size: 6643 KpnI G_GTAC'C Cuts at: 3968 3968 Size: 6643 MfeI C'AATT_G Cuts at: 3209 4242 3209 Size: 1033 5610 MluI A'CGCG_T Cuts at: 6519 6519 Size: 6643 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2137 2876 3033 3668 3897 5757 5941 1305 Size: 832 739 157 635 229 1860 184 2007 Fragments arranged by size: 2007 1860 832 739 635 229 184 157 MscI TGG'CCA Cuts at: 10 64 1489 1832 2186 10 Size: 54 1425 343 354 4467 Fragments arranged by size: 4467 1425 354 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2274 2288 3500 4601 4960 5119 518 Size: 648 1108 14 1212 1101 359 159 2042 Fragments arranged by size: 2042 1212 1108 1101 648 359 159 14 MspA1I CmG'CkG Cuts at: 1606 4059 4736 5884 6129 1606 Size: 2453 677 1148 245 2120 Fragments arranged by size: 2453 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2050 2050 Size: 6643 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5152 Fragments arranged by size: 5152 939 552 NdeI CA'TA_TG Cuts at: 387 3674 387 Size: 3287 3356 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6643 NotI GC'GGCC_GC Cuts at: 4090 4090 Size: 6643 NsiI A_TGCA'T Cuts at: 2271 2271 Size: 6643 NspI r_CATG'y Cuts at: 1132 1229 2269 2342 2515 2977 4001 5546 1132 Size: 97 1040 73 173 462 1024 1545 2229 Fragments arranged by size: 2229 1545 1040 1024 462 173 97 73 PacI TTA_AT'TAA Cuts at: 3081 3081 Size: 6643 PasI CC'CwG_GG Cuts at: 1348 2561 1348 Size: 1213 5430 PciI A'CATG_T Cuts at: 2338 5542 2338 Size: 3204 3439 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6643 PfoI T'CCnGG_A Cuts at: 2533 2533 Size: 6643 PmeI GTTT'AAAC Cuts at: 3947 3947 Size: 6643 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4712 4744 6225 6257 4712 Size: 32 1481 32 5098 Fragments arranged by size: 5098 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2056 3461 2056 Size: 1405 5238 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6643 PsiI TTA'TAA Cuts at: 3146 3588 4213 3146 Size: 442 625 5576 Fragments arranged by size: 5576 625 442 PstI C_TGCA'G Cuts at: 830 3995 830 Size: 3165 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6643 SalI G'TCGA_C Cuts at: 1936 3985 1936 Size: 2049 4594 SanDI GG'GwC_CC Cuts at: 2056 2056 Size: 6643 SapI GCTCTTCn'nnn_ Cuts at: 2139 2139 Size: 6643 SbfI CC_TGCA'GG Cuts at: 3995 3995 Size: 6643 ScaI AGT'ACT Cuts at: 4891 6603 4891 Size: 1712 4931 SfcI C'TryA_G Cuts at: 826 1045 3039 3258 3991 5126 5807 5998 826 Size: 219 1994 219 733 1135 681 191 1471 Fragments arranged by size: 1994 1471 1135 733 681 219 219 191 SfoI GGC'GCC Cuts at: 2051 2051 Size: 6643 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6643 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6643 SmaI CCC'GGG Cuts at: 1872 3970 1872 Size: 2098 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3450 4747 5648 5910 820 Size: 197 926 1507 1297 901 262 1553 Fragments arranged by size: 1553 1507 1297 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4434 493 Size: 3941 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6643 SphI G_CATG'C Cuts at: 1132 4001 1132 Size: 2869 3774 SspI AAT'ATT Cuts at: 5 52 3196 3298 3931 4567 5 Size: 47 3144 102 633 636 2081 Fragments arranged by size: 3144 2081 636 633 102 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6643 StyI C'CwwG_G Cuts at: 513 1065 2004 3803 4082 513 Size: 552 939 1799 279 3074 Fragments arranged by size: 3074 1799 939 552 279 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2300 2447 4670 4687 4840 5025 5470 Size: 1255 720 147 2223 17 153 185 445 Cuts at: 5470 325 Size: 1498 Fragments arranged by size: 2223 1498 1255 720 445 185 153 147 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2600 3323 3859 Size: 274 80 33 51 1443 623 723 536 Cuts at: 3859 4889 6601 96 Size: 1030 1712 138 Fragments arranged by size: 1712 1443 1030 723 623 536 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3096 3128 6603 6635 3096 Size: 32 3475 32 3104 Fragments arranged by size: 3475 3104 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6643 XbaI T'CTAG_A Cuts at: 3979 3979 Size: 6643 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6643 XmaI C'CCGG_G Cuts at: 1870 3968 1870 Size: 2098 4545 XmnI GAAnn'nnTTC Cuts at: 4772 4772 Size: 6643 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4451 276 Size: 53 83 186 936 2917 2468 Fragments arranged by size: 2917 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SrfI SwaI XcmI