(Circular) (Six-base) MAPSORT of: pFN21AA0015.seq Check: 4121 from: 1 to: 6067 pFN21AA0015 6067 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2422 2422 Size: 6067 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3099 3877 278 Size: 53 83 186 936 1563 778 2468 Fragments arranged by size: 2468 1563 936 778 186 83 53 AccI GT'mk_AC Cuts at: 1937 3410 1937 Size: 1473 4594 Acc65I G'GTAC_C Cuts at: 3388 3388 Size: 6067 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4130 5514 5782 1632 Size: 2498 1384 268 1917 Fragments arranged by size: 2498 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5870 AflIII A'CryG_T Cuts at: 1203 2523 3012 4966 5943 1203 Size: 1320 489 1954 977 1327 Fragments arranged by size: 1954 1327 1320 977 489 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 4796 4796 Size: 6067 AleI CACnn'nnGTG Cuts at: 1166 2774 1166 Size: 1608 4459 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6067 AlwNI CAG_nnn'CTG Cuts at: 2470 2684 3341 3810 5382 2470 Size: 214 657 469 1572 3155 Fragments arranged by size: 3155 1572 657 469 214 ApaLI G'TGCA_C Cuts at: 2642 4124 5280 2642 Size: 1482 1156 3429 Fragments arranged by size: 3429 1482 1156 ApoI r'AATT_y Cuts at: 1493 2285 3376 3602 3754 5863 1493 Size: 792 1091 226 152 2109 1697 Fragments arranged by size: 2109 1697 1091 792 226 152 AseI AT'TA_AT Cuts at: 160 1032 4621 160 Size: 872 3589 1606 Fragments arranged by size: 3589 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 2448 2819 3039 3195 3392 1870 Size: 73 505 371 220 156 197 4545 Fragments arranged by size: 4545 505 371 220 197 156 73 BamHI G'GATC_C Cuts at: 3397 3764 3397 Size: 367 5700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2434 2765 3388 4843 618 Size: 325 217 627 647 331 623 1455 1842 Fragments arranged by size: 1842 1455 647 627 623 331 325 217 BanII G_rGCy'C Cuts at: 1391 2020 2695 3056 3165 3209 3386 1391 Size: 629 675 361 109 44 177 4072 Fragments arranged by size: 4072 675 629 361 177 109 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2989 3229 928 Size: 971 1090 240 3766 Fragments arranged by size: 3766 1090 971 240 BbvCI CC'TCA_GC Cuts at: 2052 2052 Size: 6067 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4258 4292 1903 Size: 34 2321 34 3678 Fragments arranged by size: 3678 2321 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3959 5169 3959 Size: 1210 4857 BclI T'GATC_A Cuts at: 1548 1548 Size: 6067 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4678 136 Size: 107 122 71 631 605 3006 1525 Fragments arranged by size: 3006 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6062 6062 Size: 6067 BlpI GC'TnA_GC Cuts at: 2150 2181 3484 2150 Size: 31 1303 4733 Fragments arranged by size: 4733 1303 31 Bme1580I G_kGCm'C Cuts at: 1790 2646 4128 5284 1790 Size: 856 1482 1156 2573 Fragments arranged by size: 2573 1482 1156 856 BmgBI CAC'GTC Cuts at: 1318 2809 1318 Size: 1491 4576 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3798 4756 442 Size: 416 53 83 399 24 2381 958 1753 Fragments arranged by size: 2381 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3223 3223 Size: 6067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2034 2962 2971 3284 3310 4727 1239 Size: 795 928 9 313 26 1417 2579 Fragments arranged by size: 2579 1417 928 795 313 26 9 Bpu10I CC'TnA_GC Cuts at: 2052 2052 Size: 6067 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4192 5057 5355 4192 Size: 865 298 4904 Fragments arranged by size: 4904 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2051 2479 3338 4730 882 Size: 617 552 428 859 1392 2219 Fragments arranged by size: 2219 1392 859 617 552 428 BsaAI yAC'GTr Cuts at: 493 1204 2524 2641 3013 3858 493 Size: 711 1320 117 372 845 2702 Fragments arranged by size: 2702 1320 845 711 372 117 BsaBI GATnn'nnATC Cuts at: 6061 6061 Size: 6067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3096 3874 4256 4891 Size: 53 83 186 936 1563 778 382 635 Cuts at: 4891 275 Size: 1451 Fragments arranged by size: 1563 1451 936 778 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2490 4500 4898 5172 5319 5730 2490 Size: 2010 398 274 147 411 2827 Fragments arranged by size: 2827 2010 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3308 3337 3338 3367 1178 Size: 30 165 30 1905 29 1 29 3878 Fragments arranged by size: 3878 1905 165 30 30 29 29 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2174 2333 2336 2351 3191 3322 1186 Size: 883 105 159 3 15 840 131 3931 Fragments arranged by size: 3931 883 840 159 131 105 15 3 BseYI C'CCAG_C Cuts at: 2095 5270 5766 2095 Size: 3175 496 2396 Fragments arranged by size: 3175 2396 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2789 1163 Size: 573 1053 4441 Fragments arranged by size: 4441 1053 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3517 4278 5306 1540 Size: 275 186 1516 761 1028 2301 Fragments arranged by size: 2301 1516 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2151 2646 4128 4213 5284 2151 Size: 495 1482 85 1071 2934 Fragments arranged by size: 2934 1482 1071 495 85 BsmI GAATG_Cn' Cuts at: 3578 3671 3578 Size: 93 5974 BsmBI CGTCTCn'nnnn_ Cuts at: 2980 2980 Size: 6067 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2151 2646 2695 3056 3165 3209 Size: 399 230 131 495 49 361 109 44 Cuts at: 3209 3386 4128 4213 5284 1391 Size: 177 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 495 399 361 230 177 131 109 85 49 44 BspEI T'CCGG_A Cuts at: 2490 5730 2490 Size: 3240 2827 BspHI T'CATG_A Cuts at: 3956 5686 3956 Size: 1730 4337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2422 3075 3422 844 Size: 1578 653 347 3489 Fragments arranged by size: 3489 1578 653 347 BsrBI CCG'CTC Cuts at: 1394 1823 2752 2763 3202 3954 1394 Size: 429 929 11 439 752 3507 Fragments arranged by size: 3507 929 752 439 429 11 BsrDI GCAATG_nn' Cuts at: 65 1248 2787 4562 4736 65 Size: 1183 1539 1775 174 1396 Fragments arranged by size: 1775 1539 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2414 2517 4711 4898 1700 Size: 110 604 103 2194 187 2869 Fragments arranged by size: 2869 2194 604 187 110 103 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6067 BssHII G'CGCG_C Cuts at: 1925 2906 1925 Size: 981 5086 BssSI C'ACGA_G Cuts at: 4127 5139 4127 Size: 1012 5055 BstAPI GCAn_nnn'nTGC Cuts at: 794 3319 794 Size: 2525 3542 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6067 BstYI r'GATC_y Cuts at: 1972 3397 3764 4149 4166 5618 5704 5716 6062 Size: 1425 367 385 17 1452 86 12 346 Cuts at: 6062 1972 Size: 1977 Fragments arranged by size: 1977 1452 1425 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2685 3242 2685 Size: 557 5510 BtgI C'CryG_G Cuts at: 513 1065 2126 2190 513 Size: 552 1061 64 4390 Fragments arranged by size: 4390 1061 552 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5328 Fragments arranged by size: 5328 683 56 BtsI GCAGTG_nn' Cuts at: 2471 3583 4377 4397 2471 Size: 1112 794 20 4141 Fragments arranged by size: 4141 1112 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6067 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 3371 3371 Size: 6067 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2187 2416 Size: 54 1425 50 275 18 210 147 229 Cuts at: 2416 2970 3514 4403 6032 8 Size: 554 544 889 1629 43 Fragments arranged by size: 1629 1425 889 554 544 275 229 210 147 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3514 1537 Size: 275 1702 4090 Fragments arranged by size: 4090 1702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 2310 2555 3997 1353 Size: 668 289 245 1442 3423 Fragments arranged by size: 3423 1442 668 289 245 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4654 5028 5174 387 Size: 1064 138 3065 374 146 1280 Fragments arranged by size: 3065 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2962 2971 3284 3310 4130 4727 Size: 393 402 928 9 313 26 820 597 Cuts at: 4727 5514 5782 1239 Size: 787 268 1524 Fragments arranged by size: 1524 928 820 787 597 402 393 313 268 26 9 EcoO109I rG'GnC_Cy Cuts at: 2116 2134 2215 2347 2964 3247 3289 2116 Size: 18 81 132 617 283 42 4894 Fragments arranged by size: 4894 617 283 132 81 42 18 EcoRI G'AATT_C Cuts at: 2285 3376 3754 2285 Size: 1091 378 4598 Fragments arranged by size: 4598 1091 378 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 3047 5214 1678 Size: 1369 2167 2531 Fragments arranged by size: 2531 2167 1369 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3308 3337 3340 3369 4708 Size: 32 135 32 1736 29 3 29 1339 Cuts at: 4708 4740 4782 4814 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1736 1339 135 42 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 2959 3411 3657 669 Size: 988 281 1021 452 246 3079 Fragments arranged by size: 3079 1021 988 452 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6067 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KpnI G_GTAC'C Cuts at: 3392 3392 Size: 6067 MfeI C'AATT_G Cuts at: 3666 3666 Size: 6067 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5181 5365 1305 Size: 3876 184 2007 Fragments arranged by size: 3876 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2042 2189 10 Size: 54 1425 343 210 147 3888 Fragments arranged by size: 3888 1425 343 210 147 54 MslI CAynn'nnrTG Cuts at: 518 1166 2774 3227 4025 4384 4543 518 Size: 648 1608 453 798 359 159 2042 Fragments arranged by size: 2042 1608 798 648 453 359 159 MspA1I CmG'CkG Cuts at: 1606 2263 2461 2633 3159 3483 4160 5308 5553 Size: 657 198 172 526 324 677 1148 245 Cuts at: 5553 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 657 526 324 245 198 172 NaeI GCC'GGC Cuts at: 2416 2416 Size: 6067 NcoI C'CATG_G Cuts at: 513 1065 2126 2190 513 Size: 552 1061 64 4390 Fragments arranged by size: 4390 1061 552 64 NdeI CA'TA_TG Cuts at: 387 387 Size: 6067 NgoMIV G'CCGG_C Cuts at: 2414 2414 Size: 6067 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NspI r_CATG'y Cuts at: 1132 1229 3425 4970 1132 Size: 97 2196 1545 2229 Fragments arranged by size: 2229 2196 1545 97 PasI CC'CwG_GG Cuts at: 1348 2138 2301 1348 Size: 790 163 5114 Fragments arranged by size: 5114 790 163 PciI A'CATG_T Cuts at: 4966 4966 Size: 6067 PflMI CCAn_nnn'nTGG Cuts at: 1108 2377 1108 Size: 1269 4798 PfoI T'CCnGG_A Cuts at: 2070 2070 Size: 6067 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PmlI CAC'GTG Cuts at: 2524 2641 2524 Size: 117 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4136 4168 5649 5681 4136 Size: 32 1481 32 4522 Fragments arranged by size: 4522 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2116 2964 3247 3289 2116 Size: 848 283 42 4894 Fragments arranged by size: 4894 848 283 42 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6067 PsiI TTA'TAA Cuts at: 3637 3637 Size: 6067 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2564 2596 2564 Size: 32 6035 PstI C_TGCA'G Cuts at: 830 2760 3419 830 Size: 1930 659 3478 Fragments arranged by size: 3478 1930 659 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 PvuII CAG'CTG Cuts at: 2263 2263 Size: 6067 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SanDI GG'GwC_CC Cuts at: 2964 3247 3289 2964 Size: 283 42 5742 Fragments arranged by size: 5742 283 42 SapI GCTCTTCn'nnn_ Cuts at: 2021 2555 2021 Size: 534 5533 SbfI CC_TGCA'GG Cuts at: 3419 3419 Size: 6067 ScaI AGT'ACT Cuts at: 4315 6027 4315 Size: 1712 4355 SfcI C'TryA_G Cuts at: 826 1045 2267 2756 3415 4550 5231 5422 826 Size: 219 1222 489 659 1135 681 191 1471 Fragments arranged by size: 1471 1222 1135 681 659 489 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 2450 3041 3197 3394 1872 Size: 578 591 156 197 4545 Fragments arranged by size: 4545 591 578 197 156 SmlI C'TyrA_G Cuts at: 820 1017 1943 4171 5072 5334 820 Size: 197 926 2228 901 262 1553 Fragments arranged by size: 2228 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3425 1132 Size: 2293 3774 SrfI GCCC'GGGC Cuts at: 2450 2450 Size: 6067 SspI AAT'ATT Cuts at: 5 52 3991 5 Size: 47 3939 2081 Fragments arranged by size: 3939 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6067 StyI C'CwwG_G Cuts at: 513 1065 2126 2190 3506 513 Size: 552 1061 64 1316 3074 Fragments arranged by size: 3074 1316 1061 552 64 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2912 4094 4111 4264 4449 4894 325 Size: 1255 1332 1182 17 153 185 445 1498 Fragments arranged by size: 1498 1332 1255 1182 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4313 6025 96 Size: 274 80 33 51 1443 2336 1712 138 Fragments arranged by size: 2336 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2844 2876 6027 6059 2844 Size: 32 3151 32 2852 Fragments arranged by size: 3151 2852 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6067 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2197 3116 2197 Size: 919 5148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6067 XmaI C'CCGG_G Cuts at: 1870 2448 3039 3195 3392 1870 Size: 578 591 156 197 4545 Fragments arranged by size: 4545 591 578 197 156 XmnI GAAnn'nnTTC Cuts at: 4196 4196 Size: 6067 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3097 3875 276 Size: 53 83 186 936 1563 778 2468 Fragments arranged by size: 2468 1563 936 778 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI RsrII SacI SacII SexAI SfiI SfoI SwaI