ARHGEF9 ORF clone FHC00073

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00073

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Clone info.

Species

human

Clone Name

pFN21AA0424 Sequence (with vector) Restriction Map

Product ID

FHC00073

Flexi Clone PID

FXC00073

ORF Sequence

DNA sequence (1548 bp)
Protein sequence (516 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385332]

Gene

KIAA0424

Symbol

ARHGEF9

Alias : COLLYBISTIN, DEE8, EIEE8, HPEM-2, PEM-2, PEM2

Description

Cdc42 guanine nucleotide exchange factor (GEF) 9, transcript variant 1

Original Clone ID

hh01267

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1548 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 516 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS35315.1 5.5e-152 100.0 ARHGEF9 CCDS83477.1 3.6e-146 100.0 ARHGEF9 CCDS55430.1 8.2e-133 98.5 ARHGEF9 CCDS55429.1 5.9e-120 100.0 ARHGEF9 CCDS66517.1 5.8e-90 61.5 SPATA13 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR011511 23 63 PF07653 Variant SH3 IPR000219 107 286 PF00621 Dbl homology (DH) domain IPR001849 334 422 PF00169 Pleckstrin homology domain HMMSmart IPR001452 11 66 SM00326 Src homology-3 domain IPR000219 107 286 SM00325 Dbl homology (DH) domain IPR001849 319 427 SM00233 Pleckstrin homology domain ProfileScan IPR001452 8 67 PS50002 Src homology-3 domain IPR000219 103 287 PS50010 Dbl homology (DH) domain IPR001849 318 425 PS50003 Pleckstrin homology domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_056000 516 300429,300607 100.0 100.0 XP_016884855 516 300429,300607 100.0 100.0 XP_005262306 523 300429,300607 99.8 99.6 XP_011529192 516 300429,300607 99.6 100.0 XP_016884854 516 300429,300607 99.6 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA0424 Download

>KIAA0424  1548 bp
ATGACGTTGCTGATCACTGGAGATTCCATCGTTAGTGCTGAGGCAGTATGGGATCACGTC
ACCATGGCCAACCGGGAGTTGGCATTTAAAGCTGGCGACGTCATCAAAGTCTTGGATGCT
TCCAACAAGGATTGGTGGTGGGGCCAGATCGACGATGAGGAGGGATGGTTTCCTGCCAGC
TTTGTGAGGCTCTGGGTGAACCAGGAGGATGAGGTGGAGGAGGGGCCCAGCGATGTGCAG
AACGGACACCTGGACCCCAATTCAGACTGCCTCTGTCTGGGGCGGCCACTACAGAACCGG
GACCAGATGCGGGCCAATGTCATCAATGAGATAATGAGCACTGAGCGTCACTACATCAAG
CACCTCAAGGATATTTGTGAGGGCTATCTGAAGCAGTGCCGGAAGAGAAGGGACATGTTC
AGTGACGAGCAACTGAAGGTAATCTTTGGGAACATTGAAGATATCTACAGATTTCAGATG
GGCTTTGTGAGAGACCTGGAGAAACAGTATAACAATGATGACCCCCACCTCAGCGAGATA
GGACCCTGCTTCCTAGAGCACCAAGATGGATTCTGGATATACTCTGAGTATTGTAACAAC
CACCTGGATGCTTGCATGGAGCTCTCCAAACTGATGAAGGACAGCCGCTACCAGCACTTC
TTTGAGGCCTGTCGCCTCTTGCAGCAGATGATTGACATTGCTATCGATGGTTTCCTTTTG
ACTCCAGTGCAGAAGATCTGCAAGTATCCCTTACAGTTGGCTGAGCTCCTAAAGTATACT
GCCCAAGACCACAGTGACTACAGGTATGTGGCAGCTGCTTTGGCTGTCATGAGAAATGTG
ACTCAGCAGATCAACGAACGCAAGCGACGTTTAGAGAATATTGACAAGATTGCTCAGTGG
CAGGCTTCTGTCCTAGACTGGGAGGGCGAGGACATCCTAGACAGGAGCTCGGAGCTGATC
TACACTGGGGAGATGGCCTGGATCTACCAGCCCTACGGCCGCAACCAGCAGCGGGTCTTC
TTCCTGTTTGACCACCAGATGGTCCTCTGCAAGAAGGACCTAATCCGGAGAGACATCCTG
TACTACAAAGGCCGCATTGACATGGATAAATATGAGGTAGTTGACATTGAGGATGGCAGA
GATGATGACTTCAATGTCAGCATGAAGAATGCCTTTAAGCTTCACAACAAGGAGACTGAG
GAGATACATCTGTTCTTTGCCAAGAAGCTGGAGGAAAAAATACGCTGGCTCAGGGCTTTC
AGAGAAGAGAGGAAAATGGTACAGGAAGATGAAAAAATTGGCTTTGAAATTTCTGAAAAC
CAGAAGAGGCAGGCTGCAATGACTGTGAGAAAAGTCCCTAAGCAAAAAGGTGTCAACTCT
GCCCGCTCAGTTCCTCCTTCCTACCCACCACCGCAGGACCCGTTAAACCACGGCCAGTAC
CTGGTCCCCGACGGCATCGCTCAGTCGCAGGTCTTTGAGTTCACCGAACCCAAGCGCAGC
CAGTCACCATTCTGGCAAAACTTCAGCAGGTTAACCCCCTTCAAAAAA

ORF Protein

Cloned ORF protein sequence for pFN21AA0424 Download

>KIAA0424  516 aa
MTLLITGDSIVSAEAVWDHVTMANRELAFKAGDVIKVLDASNKDWWWGQIDDEEGWFPAS
FVRLWVNQEDEVEEGPSDVQNGHLDPNSDCLCLGRPLQNRDQMRANVINEIMSTERHYIK
HLKDICEGYLKQCRKRRDMFSDEQLKVIFGNIEDIYRFQMGFVRDLEKQYNNDDPHLSEI
GPCFLEHQDGFWIYSEYCNNHLDACMELSKLMKDSRYQHFFEACRLLQQMIDIAIDGFLL
TPVQKICKYPLQLAELLKYTAQDHSDYRYVAAALAVMRNVTQQINERKRRLENIDKIAQW
QASVLDWEGEDILDRSSELIYTGEMAWIYQPYGRNQQRVFFLFDHQMVLCKKDLIRRDIL
YYKGRIDMDKYEVVDIEDGRDDDFNVSMKNAFKLHNKETEEIHLFFAKKLEEKIRWLRAF
REERKMVQEDEKIGFEISENQKRQAAMTVRKVPKQKGVNSARSVPPSYPPPQDPLNHGQY
LVPDGIAQSQVFEFTEPKRSQSPFWQNFSRLTPFKK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA0424 Download

>pFN21AA0424  6253 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACGTTGCTGATCACTGGAGATTCCATCGTTAG
TGCTGAGGCAGTATGGGATCACGTCACCATGGCCAACCGGGAGTTGGCATTTAAAGCTGG
CGACGTCATCAAAGTCTTGGATGCTTCCAACAAGGATTGGTGGTGGGGCCAGATCGACGA
TGAGGAGGGATGGTTTCCTGCCAGCTTTGTGAGGCTCTGGGTGAACCAGGAGGATGAGGT
GGAGGAGGGGCCCAGCGATGTGCAGAACGGACACCTGGACCCCAATTCAGACTGCCTCTG
TCTGGGGCGGCCACTACAGAACCGGGACCAGATGCGGGCCAATGTCATCAATGAGATAAT
GAGCACTGAGCGTCACTACATCAAGCACCTCAAGGATATTTGTGAGGGCTATCTGAAGCA
GTGCCGGAAGAGAAGGGACATGTTCAGTGACGAGCAACTGAAGGTAATCTTTGGGAACAT
TGAAGATATCTACAGATTTCAGATGGGCTTTGTGAGAGACCTGGAGAAACAGTATAACAA
TGATGACCCCCACCTCAGCGAGATAGGACCCTGCTTCCTAGAGCACCAAGATGGATTCTG
GATATACTCTGAGTATTGTAACAACCACCTGGATGCTTGCATGGAGCTCTCCAAACTGAT
GAAGGACAGCCGCTACCAGCACTTCTTTGAGGCCTGTCGCCTCTTGCAGCAGATGATTGA
CATTGCTATCGATGGTTTCCTTTTGACTCCAGTGCAGAAGATCTGCAAGTATCCCTTACA
GTTGGCTGAGCTCCTAAAGTATACTGCCCAAGACCACAGTGACTACAGGTATGTGGCAGC
TGCTTTGGCTGTCATGAGAAATGTGACTCAGCAGATCAACGAACGCAAGCGACGTTTAGA
GAATATTGACAAGATTGCTCAGTGGCAGGCTTCTGTCCTAGACTGGGAGGGCGAGGACAT
CCTAGACAGGAGCTCGGAGCTGATCTACACTGGGGAGATGGCCTGGATCTACCAGCCCTA
CGGCCGCAACCAGCAGCGGGTCTTCTTCCTGTTTGACCACCAGATGGTCCTCTGCAAGAA
GGACCTAATCCGGAGAGACATCCTGTACTACAAAGGCCGCATTGACATGGATAAATATGA
GGTAGTTGACATTGAGGATGGCAGAGATGATGACTTCAATGTCAGCATGAAGAATGCCTT
TAAGCTTCACAACAAGGAGACTGAGGAGATACATCTGTTCTTTGCCAAGAAGCTGGAGGA
AAAAATACGCTGGCTCAGGGCTTTCAGAGAAGAGAGGAAAATGGTACAGGAAGATGAAAA
AATTGGCTTTGAAATTTCTGAAAACCAGAAGAGGCAGGCTGCAATGACTGTGAGAAAAGT
CCCTAAGCAAAAAGGTGTCAACTCTGCCCGCTCAGTTCCTCCTTCCTACCCACCACCGCA
GGACCCGTTAAACCACGGCCAGTACCTGGTCCCCGACGGCATCGCTCAGTCGCAGGTCTT
TGAGTTCACCGAACCCAAGCGCAGCCAGTCACCATTCTGGCAAAACTTCAGCAGGTTAAC
CCCCTTCAAAAAAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT