Vector Information

KIAA0001 sequence was obtained by assembly of 
sequences derived from two clones. ha00008 retains 
sequence from nt. positions 757 to 2416 of KIAA0001. 
The cDNA fragment was cloned by use of ZAP-cDNA 
synthesis kit and inserted at the EcoRI-XhoI site of 
the pNTT104 vector (GenBank Accession No. AB032066, AmpR).
The 5' end of the cDNA was repaired and ligated with 
the EcoRI adapter (5'AATTCGGCACGAG3') and inserted 
at the EcoRI site of the vector. The 3' end of the cDNA 
was inserted at the XhoI site of the vector. The other 
clone a240 harbors sequence from nt. positions 1 to 
795 of KIAA0001 which was amplified by RACE and 
cloned into M13mp18 vector (GenBank Accession No. 
X02513).
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