##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI AocI Aor51HI ApaI
AscI Asp718I AspEI AviII AvrII
BanI BanII BbeI BglI BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BsrFI BssHII BssSI BstAPI
BstUI Bsu36I CelII Cfr10I ClaI
CpoI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoT22I
FseI FspI HgaI Hin1I KasI
KpnI KspI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PinAI PmeI
PshAI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
Sse8387I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AclI 2456 AACGTT AA^CGTT
Alw44I 2523 GTGCAC G^TGCAC
ApaLI 2523 GTGCAC G^TGCAC
AspHI 2523 GTGCAC G[AT]GC[AT]^C
AvaI 949 CCCGAG C^[CT]CG[GA]G
BglII 2995 AGATCT A^GATCT
BsaBI 4263 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 94 CTCCTC GAGGAG(10/8)
BsiHKAI 2523 GTGCAC G[AT]GC[AT]^C
BsoBI 949 CCCGAG C^[CT]CG[GA]G
Bsp1407I 420 TGTACA T^GTACA
BspHI 3687 TCATGA T^CATGA
BspLU11I 2361 ACATGT A^CATGT
BspMI 218 ACCTGC ACCTGC(4/8)
BsrGI 420 TGTACA T^GTACA
Bst1107I 2681 GTATAC GTA^TAC
BstEII 559 GGTTACC G^GT[ACGT]ACC
BstPI 559 GGTTACC G^GT[ACGT]ACC
BstZ17I 2681 GTATAC GTA^TAC
CfoI 3452 GCGC GCG^C
DrdI 1901 GACCTCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 559 GGTTACC G^GT[ACGT]ACC
FauI 3650 GCGGG CCCGC(4/6)
HaeII 3451 AGCGCC [GA]GCGC^[CT]
HhaI 3452 GCGC GCG^C
HinP1I 3452 GCGC G^CGC
MamI 4263 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflMI 4257 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1455 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 2456 AACGTT AA^CGTT
RcaI 3687 TCATGA T^CATGA
SapI 2118 GCTCTTC GCTCTTC(1/4)
SspBI 420 TGTACA T^GTACA
StuI 3236 AGGCCT AGG^CCT
SwaI 4043 ATTTAAAT ATTT^AAAT
Van91I 4257 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 641 CCATCAGCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 421 GTAC GT^AC
1384 GTAC
AflII 2353 CTTAAG C^TTAAG
2922 CTTAAG
AlwNI 99 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
661 CAGCCACTG
BbrPI 3468 CACGTG CAC^GTG
3504 CACGTG
BbvCI 87 CCTCAGC CCTCAGC(-5/-2)
940 CCTCAGC
BcgI 76 CGATCCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
929 CGATTTTCCTGC
BclI 763 TGATCA T^GATCA
3096 TGATCA
BcnI 268 CCCGG CC^[GC]GG
1667 CCCGG
BfrI 2353 CTTAAG C^TTAAG
2922 CTTAAG
BmyI 1288 GTGCCC G[AGT]GC[ACT]^C
2523 GTGCAC
BsaI 4288 GAGACC GGTCTC(1/5)
1042 GGTCTC
Bsp1286I 1288 GTGCCC G[AGT]GC[ACT]^C
2523 GTGCAC
BsrDI 900 CATTGC GCAATG(2/0)
2319 GCAATG
BstBI 3104 TTCGAA TT^CGAA
3680 TTCGAA
BstXI 233 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2023 CCAGAAGAATGG
DraI 3018 TTTAAA TTT^AAA
4044 TTTAAA
DraII 1455 GGGACCC [GA]G^G[ACGT]CC[CT]
3208 AGGCCCT
EcoO109I 1455 GGGACCC [GA]G^G[ACGT]CC[CT]
3208 AGGCCCT
EcoRV 305 GATATC GAT^ATC
2111 GATATC
FbaI 763 TGATCA T^GATCA
3096 TGATCA
HapII 269 CCGG C^CGG
1668 CCGG
HindIII 2189 AAGCTT A^AGCTT
2537 AAGCTT
HpaI 534 GTTAAC GTT^AAC
3511 GTTAAC
HpaII 269 CCGG C^CGG
1668 CCGG
MspI 269 CCGG C^CGG
1668 CCGG
NciI 268 CCCGG CC^[GC]GG
1667 CCCGG
NdeI 2932 CATATG CA^TATG
3847 CATATG
NspV 3104 TTCGAA TT^CGAA
3680 TTCGAA
PmaCI 3468 CACGTG CAC^GTG
3504 CACGTG
PmlI 3468 CACGTG CAC^GTG
3504 CACGTG
RsaI 421 GTAC GT^AC
1384 GTAC
SexAI 1573 ACCAGGT A^CC[AT]GGT
2824 ACCAGGT
SfuI 3104 TTCGAA TT^CGAA
3680 TTCGAA
SspI 1730 AATATT AAT^ATT
2758 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccI AciI AcsI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AspI AvaII BalI BamHI BbsI
BbvI BciVI BfaI BpmI BpuAI
BsaAI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsmI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EaeI EarI EcoNI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PflFI
PleI PshBI PstI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII XmnI