##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AccIII AclI AflII AgeI AocI
ApaI AscI AvrII BbeI BbrPI
BcgI BciVI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BsrFI BsrGI BssHII BstBI
BstEII BstPI Bsu36I CelII Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco52I Eco81I EcoO65I EcoRI FseI
HgaI KasI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2307 GGTACC G^GTACC
AccII 2006 CGCG CG^CG
AhdI 1495 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4177 AGCGCT AGC^GCT
Asp700I 932 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2307 GGTACC G^GTACC
AspEI 1495 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1966 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3179 TGCGCA TGC^GCA
BamHI 3897 GGATCC G^GATCC
BanII 3779 GAGCTC G[GA]GC[CT]^C
BbvCI 3410 GCTGAGG CCTCAGC(-5/-2)
BclI 107 TGATCA T^GATCA
BsmI 1167 GCATTC GAATGC(1/-1)
Bst1107I 1730 GTATAC GTA^TAC
BstUI 2006 CGCG CG^CG
BstZ17I 1730 GTATAC GTA^TAC
Eam1105I 1495 GACCTTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4177 AGCGCT AGC^GCT
EcoRV 1231 GATATC GAT^ATC
EcoT22I 1644 ATGCAT ATGCA^T
FbaI 107 TGATCA T^GATCA
FspI 3179 TGCGCA TGC^GCA
HpaI 2149 GTTAAC GTT^AAC
KpnI 2307 GGTACC GGTAC^C
MvnI 2006 CGCG CG^CG
NsiI 1644 ATGCAT ATGCA^T
PaeR7I 1767 CTCGAG C^TCGAG
PflFI 1966 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4276 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 3569 CAGCTG CAG^CTG
SacI 3779 GAGCTC GAGCT^C
SapI 814 GCTCTTC GCTCTTC(1/4)
ScaI 4373 AGTACT AGT^ACT
SfiI 4471 GGCCCTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 766 CCCGGG CCC^GGG
Tth111I 1966 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1767 CTCGAG C^TCGAG
XmaCI 766 CCCGGG C^CCGGG
XmaI 766 CCCGGG C^CCGGG
XmnI 932 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 612 GTCTAC GT^[AC][GT]AC
1730 GTATAC
AflIII 1698 ACATGT A^C[GA][CT]GT
1734 ACATGT
BanI 2307 GGTACC G^G[CT][GA]CC
3728 GGCACC
BbsI 77 GAAGAC GAAGAC(2/6)
3016 GAAGAC
BglII 329 AGATCT A^GATCT
2669 AGATCT
BpuAI 77 GAAGAC GAAGAC(2/6)
3016 GAAGAC
BsmBI 451 CGTCTC CGTCTC(1/5)
4065 GAGACG
BspLU11I 1698 ACATGT A^CATGT
1734 ACATGT
BsrBI 2956 GAGCGG CCGCTC(-3/-3)
3488 GAGCGG
BssSI 2178 CTCGTG CACGAG(-5/-1)
2554 CTCGTG
BstAPI 1704 GCACAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3967 GCACCTGCTGC
BstXI 3146 CCAGGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3216 CCAAGGACATGG
EcoNI 55 CCTTTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3211 CCTATCCAAGG
MspA1I 3569 CAGCTG C[AC]G^C[GT]G
3984 CCGCTG
NcoI 2865 CCATGG C^CATGG
3591 CCATGG
PacI 1866 TTAATTAA TTAAT^TAA
1870 TTAATTAA
PpuMI 2805 AGGACCT [GA]G^G[AT]CC[CT]
3993 AGGACCT
SmlI 774 CTTGAG C^T[CT][GA]AG
1767 CTCGAG
SphI 1642 GCATGC GCATG^C
2985 GCATGC
SspI 2566 AATATT AAT^ATT
3098 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AatII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI AseI AsnI AspHI AvaI
AvaII BalI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PshBI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII