##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspI        AvrII       BamHI       BbeI        
BbrPI       BbsI        BciVI       BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        EagI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
FauI        FseI        HaeII       Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
Sse8387I    SwaI        Tth111I     Van91I      XcmI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         405  CGCG                 CG^CG
AhdI         2568  GACATGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1377  CCTTAGG              CC^T[ACGT]AGG
AspEI        2568  GACATGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII        2243  GGACC                G^G[AT]CC
AviII        1953  TGCGCA               TGC^GCA
BbvCI        2784  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2773  CGATTCTCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2218  TGATCA               T^GATCA
BsaBI        2892  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrBI        3491  CCGCTC               CCGCTC(-3/-3)
BssSI        2610  CACGAG               CACGAG(-5/-1)
BstAPI        459  GCAGAAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I       3216  CATCC                GGATG(2/0)
BstUI         405  CGCG                 CG^CG
BstXI        3188  CCAGTGTTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        2798  GGATCT               [GA]^GATC[CT]
Bsu36I       1377  CCTTAGG              CC^T[ACGT]AGG
DraIII         88  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3291  GACCCTGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         2948  CCGTGG               C^C[GA][CT]GG
Eam1105I     2568  GACATGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1377  CCTTAGG              CC^T[ACGT]AGG
FbaI         2218  TGATCA               T^GATCA
FokI         3216  CATCC                GGATG(9/13)
FspI         1953  TGCGCA               TGC^GCA
HgaI          872  GCGTC                GACGC(5/10)
MamI         2892  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         2798  GGATCT               [GA]^GATC[CT]
MspA1I        750  CAGCTG               C[AC]G^C[GT]G
MvnI          405  CGCG                 CG^CG
NdeI         1583  CATATG               CA^TATG
PaeR7I       1982  CTCGAG               C^TCGAG
PmeI          124  GTTTAAAC             GTTT^AAAC
PpuMI        2242  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII         750  CAGCTG               CAG^CTG
SmaI         2793  CCCGGG               CCC^GGG
SpeI         1234  ACTAGT               A^CTAGT
SphI         3449  GCATGC               GCATG^C
SspI         2423  AATATT               AAT^ATT
XbaI          817  TCTAGA               T^CTAGA
XhoI         1982  CTCGAG               C^TCGAG
XhoII        2798  GGATCT               [GA]^GATC[CT]
XmaCI        2793  CCCGGG               C^CCGGG
XmaI         2793  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AflIII        786  ACACGT               A^C[GA][CT]GT
             2411  ACACGT              
AlwNI         460  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2499  CAGCATCTG           
AspHI        1788  GAGCTC               G[AT]GC[AT]^C
             2310  GAGCTC              
AvaI         1982  CTCGAG               C^[CT]CG[GA]G
             2793  CCCGGG              
BalI         2685  TGGCCA               TGG^CCA
             3348  TGGCCA              
BanI          303  GGCACC               G^G[CT][GA]CC
             1288  GGCACC              
BanII        1788  GAGCTC               G[GA]GC[CT]^C
             2310  GAGCTC              
BsaAI         787  CACGTA               [CT]AC^GT[GA]
             2412  CACGTA              
BsiHKAI      1788  GAGCTC               G[AT]GC[AT]^C
             2310  GAGCTC              
BsmI          760  GAATGC               GAATGC(1/-1)
             1915  GAATGC              
BsoBI        1982  CTCGAG               C^[CT]CG[GA]G
             2793  CCCGGG              
Bsp1407I     2408  TGTACA               T^GTACA
             3163  TGTACA              
BspMI         361  GCAGGT               ACCTGC(4/8)
             2616  GCAGGT              
BsrGI        2408  TGTACA               T^GTACA
             3163  TGTACA              
CfoI          404  GCGC                 GCG^C
             1954  GCGC                
DraI          125  TTTAAA               TTT^AAA
             1960  TTTAAA              
EcoNI         204  CCTTAGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              355  CCTGGGGCAGG         
EcoT22I       762  ATGCAT               ATGCA^T
             2854  ATGCAT              
HapII        2552  CCGG                 C^CGG
             2794  CCGG                
HhaI          404  GCGC                 GCG^C
             1954  GCGC                
HinP1I        404  GCGC                 G^CGC
             1954  GCGC                
HpaII        2552  CCGG                 C^CGG
             2794  CCGG                
MluNI        2685  TGGCCA               TGG^CCA
             3348  TGGCCA              
MscI         2685  TGGCCA               TGG^CCA
             3348  TGGCCA              
MspI         2552  CCGG                 C^CGG
             2794  CCGG                
NsiI          762  ATGCAT               ATGCA^T
             2854  ATGCAT              
NspI         2903  GCATGT               [GA]CATG^[CT]
             3449  GCATGC              
SacI         1788  GAGCTC               GAGCT^C
             2310  GAGCTC              
SspBI        2408  TGTACA               T^GTACA
             3163  TGTACA              
TfiI          525  GAATC                G^A[AT]TC
             2774  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     BbvI        BcnI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      HaeIII      
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PstI        
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StuI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XmnI