##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AgeI Alw44I Aor51HI ApaLI
AscI Asp718I AspI AviII AvrII
BalI BbsI BciVI BlnI BlpI
Bpu1102I BpuAI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrGI BssHII
Bst1107I BstEII BstPI BstZ17I CelII
ClaI CpoI DrdI Eco47III EcoO65I
EcoT22I FseI FspI HindIII KpnI
MluI MluNI MscI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmeI PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfiI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 912 GACGTC GACGT^C
AccI 1003 GTAGAC GT^[AC][GT]AC
AccIII 4795 TCCGGA T^CCGGA
AclI 937 AACGTT AA^CGTT
AflII 146 CTTAAG C^TTAAG
AhdI 2191 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2930 GGGCCC GGGCC^C
AspEI 2191 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2306 GGATCC G^GATCC
BbeI 1701 GGCGCC GGCGC^C
BbrPI 4075 CACGTG CAC^GTG
BclI 502 TGATCA T^GATCA
BfrI 146 CTTAAG C^TTAAG
BglI 1907 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 4795 TCCGGA [AT]^CCGG[AT]
BseAI 4795 TCCGGA T^CCGGA
BspEI 4795 TCCGGA T^CCGGA
BsrDI 977 GCAATG GCAATG(2/0)
BssSI 2800 CTCGTG CACGAG(-5/-1)
EaeI 1593 CGGCCG [CT]^GGCC[GA]
EagI 1593 CGGCCG C^GGCCG
Eam1105I 2191 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1593 CGGCCG C^GGCCG
Eco52I 1593 CGGCCG C^GGCCG
EcoNI 1506 CCTGCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4652 GAATTC G^AATTC
EcoRV 3359 GATATC GAT^ATC
EcoT14I 1522 CCTTGG C^C[AT][AT]GG
FbaI 502 TGATCA T^GATCA
KasI 1701 GGCGCC G^GCGCC
MfeI 2492 CAATTG C^AATTG
MroI 4795 TCCGGA T^CCGGA
MunI 2492 CAATTG C^AATTG
NarI 1701 GGCGCC GG^CGCC
PflMI 222 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4075 CACGTG CAC^GTG
PmlI 4075 CACGTG CAC^GTG
Psp1406I 937 AACGTT AA^CGTT
SacI 3114 GAGCTC GAGCT^C
SapI 98 GAAGAGC GCTCTTC(1/4)
SfoI 1701 GGCGCC GGC^GCC
SgrAI 2564 CGCCGGTG C[GA]^CCGG[CT]G
SphI 2795 GCATGC GCATG^C
StyI 1522 CCTTGG C^C[AT][AT]GG
Van91I 222 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5080 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 1787 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
4711 CAGCAACTG
AocI 2157 CCTCAGG CC^T[ACGT]AGG
2811 CCTGAGG
BanI 1701 GGCGCC G^G[CT][GA]CC
3034 GGCACC
BbvCI 2620 CCTCAGC CCTCAGC(-5/-2)
2331 GCTGAGG
BsaBI 1833 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3355 GATAGATATC
BsiEI 248 CGGTCG CG[GA][CT]^CG
1593 CGGCCG
BsmI 1581 GAATGC GAATGC(1/-1)
4955 GCATTC
BspMI 1392 ACCTGC ACCTGC(4/8)
2327 ACCTGC
BsrFI 2565 GCCGGT [GA]^CCGG[CT]
4367 ACCGGC
BstAPI 196 GCAGCGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
655 GCACTACGTGC
BstBI 1247 TTCGAA TT^CGAA
4844 TTCGAA
BstXI 1819 CCAGGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3076 CCATCAGCCTGG
Bsu36I 2157 CCTCAGG CC^T[ACGT]AGG
2811 CCTGAGG
Cfr10I 2565 GCCGGT [GA]^CCGG[CT]
4367 ACCGGC
Eco81I 2157 CCTCAGG CC^T[ACGT]AGG
2811 CCTGAGG
HaeII 218 AGCGCC [GA]GCGC^[CT]
1701 GGCGCC
KspI 356 CCGCGG CCGC^GG
3310 CCGCGG
MamI 1833 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3355 GATAGATATC
NspI 463 ACATGC [GA]CATG^[CT]
2795 GCATGC
NspV 1247 TTCGAA TT^CGAA
4844 TTCGAA
PshAI 692 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1660 GACTTGTGTC
SacII 356 CCGCGG CCGC^GG
3310 CCGCGG
SfuI 1247 TTCGAA TT^CGAA
4844 TTCGAA
XcmI 2732 CCATCTCGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4859 CCAATGTAACTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
BanII BbvI BcgI BcnI BfaI
BglII BmyI BpmI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI XmnI