##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AviII BamHI BbeI
BbrPI BciVI BglI BlpI Bpu1102I
BsaAI BsaHI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoT22I FseI FspI HaeII HgaI
Hin1I HpaI KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI XbaI XcmI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 394 GTAGAC GT^[AC][GT]AC
AccII 2113 CGCG CG^CG
AflIII 1612 ACATGT A^C[GA][CT]GT
AhdI 2055 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1952 GTGCAC G^TGCAC
ApaI 1923 GGGCCC GGGCC^C
ApaLI 1952 GTGCAC G^TGCAC
AspEI 2055 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4162 CCTAGG C^CTAGG
BcgI 3391 GCATTTCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 4249 CCCGG CC^[GC]GG
BlnI 4162 CCTAGG C^CTAGG
BsaBI 1383 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1283 ACCGGA [AT]^CCGG[AT]
BsmBI 4140 GAGACG CGTCTC(1/5)
BspHI 3408 TCATGA T^CATGA
BspLU11I 1612 ACATGT A^CATGT
BsrBI 3492 GAGCGG CCGCTC(-3/-3)
BssSI 1074 CACGAG CACGAG(-5/-1)
BstEII 2497 GGTCACC G^GT[ACGT]ACC
BstPI 2497 GGTCACC G^GT[ACGT]ACC
BstUI 2113 CGCG CG^CG
CfoI 4084 GCGC GCG^C
DraIII 3501 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2055 GACTGAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1218 CCTGGTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2497 GGTCACC G^GT[ACGT]ACC
EcoRI 88 GAATTC G^AATTC
EcoRV 1345 GATATC GAT^ATC
HhaI 4084 GCGC GCG^C
HinP1I 4084 GCGC G^CGC
HincII 1600 GTCAAC GT[CT]^[GA]AC
HindII 1600 GTCAAC GT[CT]^[GA]AC
HindIII 2646 AAGCTT A^AGCTT
MamI 1383 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2113 CGCG CG^CG
NciI 4249 CCCGG CC^[GC]GG
NheI 3680 GCTAGC G^CTAGC
PflMI 1524 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 3408 TCATGA T^CATGA
SspI 2887 AATATT AAT^ATT
Van91I 1524 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 88 GAATTC [GA]^AATT[CT]
1692 GAATTT
AocI 751 CCTCAGG CC^T[ACGT]AGG
3454 CCTGAGG
ApoI 88 GAATTC [GA]^AATT[CT]
1692 GAATTT
BalI 467 TGGCCA TGG^CCA
1638 TGGCCA
BbsI 2052 GAAGAC GAAGAC(2/6)
2342 GTCTTC
BglII 209 AGATCT A^GATCT
1511 AGATCT
BpuAI 2052 GAAGAC GAAGAC(2/6)
2342 GTCTTC
BsrDI 180 CATTGC GCAATG(2/0)
1758 CATTGC
Bsu36I 751 CCTCAGG CC^T[ACGT]AGG
3454 CCTGAGG
DraI 3158 TTTAAA TTT^AAA
4267 TTTAAA
EaeI 467 TGGCCA [CT]^GGCC[GA]
1638 TGGCCA
EarI 313 GAAGAG CTCTTC(1/4)
2742 GAAGAG
Eco81I 751 CCTCAGG CC^T[ACGT]AGG
3454 CCTGAGG
HapII 1284 CCGG C^CGG
4250 CCGG
HpaII 1284 CCGG C^CGG
4250 CCGG
Ksp632I 313 GAAGAG CTCTTC(1/4)
2742 GAAGAG
MaeII 140 ACGT A^CGT
1352 ACGT
MluNI 467 TGGCCA TGG^CCA
1638 TGGCCA
MscI 467 TGGCCA TGG^CCA
1638 TGGCCA
MspI 1284 CCGG C^CGG
4250 CCGG
SexAI 1217 ACCTGGT A^CC[AT]GGT
1325 ACCTGGT
SphI 3296 GCATGC GCATG^C
4080 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AflII AluI AlwI
AlwNI AspHI AspI AvaI AvaII
BanI BanII BbvCI BbvI BclI
BfaI BfrI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV NspI
PaeR7I PflFI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoI XhoII