##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AclI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AspEI BamHI
BbrPI BcgI BfrI BglII BsaAI
BsiWI BsmI Bsp1407I BspDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
Eam1105I Eco47III EcoNI EcoO65I EcoRV
EcoT22I FseI HindIII HpaI KspI
MfeI MluI MunI NaeI NdeI
NgoMIV NheI NotI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SapI ScaI SfiI
SfuI SnaBI SpeI SphI SspBI
StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 663 GGTACC G^GTACC
AccI 107 GTCTAC GT^[AC][GT]AC
AocI 595 CCTGAGG CC^T[ACGT]AGG
AseI 2365 ATTAAT AT^TAAT
AsnI 2365 ATTAAT AT^TAAT
Asp700I 2555 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 663 GGTACC G^GTACC
AspI 1679 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 828 TGCGCA TGC^GCA
AvrII 667 CCTAGG C^CTAGG
BalI 2121 TGGCCA TGG^CCA
BbeI 1271 GGCGCC GGCGC^C
BbvCI 1538 GCTGAGG CCTCAGC(-5/-2)
BciVI 892 GTATCC GTATCC(6/5)
BclI 2387 TGATCA T^GATCA
BglI 1323 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 667 CCTAGG C^CTAGG
BsaBI 55 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 2056 CGGCCG CG[GA][CT]^CG
BspHI 1960 TCATGA T^CATGA
BsrDI 325 CATTGC GCAATG(2/0)
BstAPI 1237 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 595 CCTGAGG CC^T[ACGT]AGG
DraIII 1718 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2056 CGGCCG C^GGCCG
EclXI 2056 CGGCCG C^GGCCG
Eco52I 2056 CGGCCG C^GGCCG
Eco81I 595 CCTGAGG CC^T[ACGT]AGG
EcoRI 2559 GAATTC G^AATTC
FbaI 2387 TGATCA T^GATCA
FspI 828 TGCGCA TGC^GCA
HaeII 1271 GGCGCC [GA]GCGC^[CT]
HincII 935 GTTGAC GT[CT]^[GA]AC
HindII 935 GTTGAC GT[CT]^[GA]AC
KasI 1271 GGCGCC G^GCGCC
KpnI 663 GGTACC GGTAC^C
MamI 55 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2121 TGGCCA TGG^CCA
MscI 2121 TGGCCA TGG^CCA
MslI 80 CACCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1271 GGCGCC GG^CGCC
NcoI 1008 CCATGG C^CATGG
NruI 820 TCGCGA TCG^CGA
PaeR7I 1206 CTCGAG C^TCGAG
PflFI 1679 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2365 ATTAAT AT^TAAT
RcaI 1960 TCATGA T^CATGA
SfoI 1271 GGCGCC GGC^GCC
SgrAI 1511 CACCGGCG C[GA]^CCGG[CT]G
Tth111I 1679 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 744 CCAACGGAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1206 CTCGAG C^TCGAG
XmnI 2555 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 104 GACGTC GACGT^C
764 GACGTC
AccIII 2451 TCCGGA T^CCGGA
2551 TCCGGA
AcsI 645 AAATTC [GA]^AATT[CT]
2559 GAATTC
ApoI 645 AAATTC [GA]^AATT[CT]
2559 GAATTC
AspHI 143 GAGCAC G[AT]GC[AT]^C
1954 GAGCAC
BbsI 551 GTCTTC GAAGAC(2/6)
1778 GTCTTC
BpuAI 551 GTCTTC GAAGAC(2/6)
1778 GTCTTC
BseAI 2451 TCCGGA T^CCGGA
2551 TCCGGA
BsgI 1373 CTGCAC GTGCAG(16/14)
1976 GTGCAG
BsiHKAI 143 GAGCAC G[AT]GC[AT]^C
1954 GAGCAC
BsmBI 148 CGTCTC CGTCTC(1/5)
1466 CGTCTC
BspEI 2451 TCCGGA T^CCGGA
2551 TCCGGA
BsrFI 1512 ACCGGC [GA]^CCGG[CT]
2263 ACCGGC
BstYI 1097 AGATCC [GA]^GATC[CT]
2448 AGATCC
Cfr10I 1512 ACCGGC [GA]^CCGG[CT]
2263 ACCGGC
DrdI 758 GACCCCGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1558 GACGCCAGAGTC
EarI 473 CTCTTC CTCTTC(1/4)
2491 CTCTTC
Ksp632I 473 CTCTTC CTCTTC(1/4)
2491 CTCTTC
MflI 1097 AGATCC [GA]^GATC[CT]
2448 AGATCC
MroI 2451 TCCGGA T^CCGGA
2551 TCCGGA
MseI 417 TTAA T^TAA
2366 TTAA
PflMI 523 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
704 CCAGCCCCTGG
SbfI 349 CCTGCAGG CCTGCA^GG
2274 CCTGCAGG
SexAI 132 ACCTGGT A^CC[AT]GGT
174 ACCTGGT
SmlI 92 CTCAAG C^T[CT][GA]AG
1206 CTCGAG
Sse8387I 349 CCTGCAGG CCTGCA^GG
2274 CCTGCAGG
SspI 123 AATATT AAT^ATT
1633 AATATT
TfiI 959 GATTC G^A[AT]TC
2211 GAATC
Tru9I 417 TTAA T^TAA
2366 TTAA
Van91I 523 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
704 CCAGCCCCTGG
XhoII 1097 AGATCC [GA]^GATC[CT]
2448 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BsaWI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrI
BssKI BssSI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XmaCI
XmaI