##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BamHI
BbvCI BciVI BclI BlnI BlpI
Bpu1102I BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI BssSI Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DraIII Eco47III EcoRV FbaI FseI
HpaI KpnI KspI MfeI MluI
MroI MunI NdeI NotI NruI
NspV PacI PflFI PmeI PshBI
Psp1406I RcaI RsrII SacII SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 690 GTCGAC GT^[AC][GT]AC
AcsI 1461 GAATTC [GA]^AATT[CT]
AflII 1595 CTTAAG C^TTAAG
AflIII 2548 ACATGT A^C[GA][CT]GT
AgeI 963 ACCGGT A^CCGGT
AhdI 1340 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 774 CCTCAGG CC^T[ACGT]AGG
ApaI 1668 GGGCCC GGGCC^C
ApoI 1461 GAATTC [GA]^AATT[CT]
AspEI 1340 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3183 TGCGCA TGC^GCA
BbeI 1654 GGCGCC GGCGC^C
BbrPI 1965 CACGTG CAC^GTG
BbsI 710 GAAGAC GAAGAC(2/6)
BfrI 1595 CTTAAG C^TTAAG
BglII 1425 AGATCT A^GATCT
BpuAI 710 GAAGAC GAAGAC(2/6)
BsaBI 1546 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1810 GAGACG CGTCTC(1/5)
BspLU11I 2548 ACATGT A^CATGT
BstAPI 141 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 774 CCTCAGG CC^T[ACGT]AGG
DraI 2276 TTTAAA TTT^AAA
DrdI 1036 GACCTCACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1728 CCATGG C^C[GA][CT]GG
EagI 2944 CGGCCG C^GGCCG
Eam1105I 1340 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2944 CGGCCG C^GGCCG
Eco52I 2944 CGGCCG C^GGCCG
Eco81I 774 CCTCAGG CC^T[ACGT]AGG
EcoRI 1461 GAATTC G^AATTC
FspI 3183 TGCGCA TGC^GCA
KasI 1654 GGCGCC G^GCGCC
MamI 1546 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1654 GGCGCC GG^CGCC
NcoI 1728 CCATGG C^CATGG
NheI 1816 GCTAGC G^CTAGC
PaeR7I 1937 CTCGAG C^TCGAG
PinAI 963 ACCGGT A^CCGGT
PmaCI 1965 CACGTG CAC^GTG
PmlI 1965 CACGTG CAC^GTG
PpuMI 1747 GGGTCCC [GA]G^G[AT]CC[CT]
PvuI 1235 CGATCG CGAT^CG
SacI 2452 GAGCTC GAGCT^C
SalI 690 GTCGAC G^TCGAC
ScaI 24 AGTACT AGT^ACT
SfoI 1654 GGCGCC GGC^GCC
SmaI 254 CCCGGG CCC^GGG
SphI 1222 GCATGC GCATG^C
TfiI 2638 GAATC G^A[AT]TC
XcmI 1531 CCAGGAAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1937 CTCGAG C^TCGAG
XmaCI 254 CCCGGG C^CCGGG
XmaI 254 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 1654 GGCGCC G[GA]^CG[CT]C
2150 GGCGTC
AlwNI 392 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1170 CAGACACTG
BalI 388 TGGCCA TGG^CCA
2624 TGGCCA
BsaAI 1965 CACGTG [CT]AC^GT[GA]
2652 CACGTA
BsaHI 1654 GGCGCC G[GA]^CG[CT]C
2150 GGCGTC
BsaWI 963 ACCGGT [AT]^CCGG[AT]
1491 ACCGGA
BsmI 284 GAATGC GAATGC(1/-1)
463 GAATGC
BspMI 1774 ACCTGC ACCTGC(4/8)
1757 GCAGGT
BsrBI 2400 CCGCTC CCGCTC(-3/-3)
2375 GAGCGG
BsrDI 3180 CATTGC GCAATG(2/0)
3230 GCAATG
BstEII 1044 GGTCACC G^GT[ACGT]ACC
2505 GGTAACC
BstPI 1044 GGTCACC G^GT[ACGT]ACC
2505 GGTAACC
BstXI 1479 CCAAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2627 CCAAAGCCCTGG
EcoNI 70 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
337 CCTGTCCCAGG
EcoO65I 1044 GGTCACC G^GT[ACGT]ACC
2505 GGTAACC
EcoT14I 405 CCTTGG C^C[AT][AT]GG
1728 CCATGG
EcoT22I 286 ATGCAT ATGCA^T
3274 ATGCAT
HgaI 2108 GCGTC GACGC(5/10)
2151 GCGTC
Hin1I 1654 GGCGCC G[GA]^CG[CT]C
2150 GGCGTC
HincII 690 GTCGAC GT[CT]^[GA]AC
2182 GTCAAC
HindII 690 GTCGAC GT[CT]^[GA]AC
2182 GTCAAC
HindIII 806 AAGCTT A^AGCTT
1255 AAGCTT
MluNI 388 TGGCCA TGG^CCA
2624 TGGCCA
MscI 388 TGGCCA TGG^CCA
2624 TGGCCA
MslI 1639 CACTGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1898 CACACAGGTG
NaeI 237 GCCGGC GCC^GGC
1778 GCCGGC
NgoMIV 237 GCCGGC G^CCGGC
1778 GCCGGC
NsiI 286 ATGCAT ATGCA^T
3274 ATGCAT
PshAI 1509 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2175 GACAAATGTC
PstI 921 CTGCAG CTGCA^G
1909 CTGCAG
SapI 1279 GAAGAGC GCTCTTC(1/4)
2835 GCTCTTC
StyI 405 CCTTGG C^C[AT][AT]GG
1728 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII