##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AflIII ApoI AscI AseI AsnI
Asp718I BclI BfrI BsaBI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI Bst1107I BstBI BstZ17I ClaI
DraI EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PshBI Psp1406I PvuI
RcaI SalI SbfI ScaI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2634 GTCTAC GT^[AC][GT]AC
AccIII 4774 TCCGGA T^CCGGA
AgeI 849 ACCGGT A^CCGGT
Alw44I 1613 GTGCAC G^TGCAC
Aor51HI 3168 AGCGCT AGC^GCT
ApaLI 1613 GTGCAC G^TGCAC
AviII 4811 TGCGCA TGC^GCA
AvrII 271 CCTAGG C^CTAGG
BciVI 4105 GTATCC GTATCC(6/5)
BglII 3181 AGATCT A^GATCT
BlnI 271 CCTAGG C^CTAGG
BsaI 5274 GGTCTC GGTCTC(1/5)
BseAI 4774 TCCGGA T^CCGGA
BspEI 4774 TCCGGA T^CCGGA
BsrDI 5113 GCAATG GCAATG(2/0)
BssHII 4011 GCGCGC G^CGCGC
CpoI 4864 CGGACCG CG^G[AT]CCG
Eco47III 3168 AGCGCT AGC^GCT
FspI 4811 TGCGCA TGC^GCA
MroI 4774 TCCGGA T^CCGGA
PflMI 2180 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 849 ACCGGT A^CCGGT
PshAI 846 GACACCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 4864 CGGACCG CG^G[AT]CCG
SapI 3506 GCTCTTC GCTCTTC(1/4)
SexAI 1622 ACCTGGT A^CC[AT]GGT
Van91I 2180 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AhdI 716 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
918 GACCTGGAGTC
Asp700I 2222 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5207 GAATTGATTC
AspEI 716 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
918 GACCTGGAGTC
AspI 1950 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4896 GACTTTGTC
BalI 3958 TGGCCA TGG^CCA
4724 TGGCCA
BamHI 561 GGATCC G^GATCC
1018 GGATCC
BbrPI 1064 CACGTG CAC^GTG
4716 CACGTG
BbsI 1089 GTCTTC GAAGAC(2/6)
4399 GTCTTC
BlpI 251 GCTGAGC GC^T[ACGT]AGC
1477 GCTCAGC
Bpu1102I 251 GCTGAGC GC^T[ACGT]AGC
1477 GCTCAGC
BpuAI 1089 GTCTTC GAAGAC(2/6)
4399 GTCTTC
BsaWI 849 ACCGGT [AT]^CCGG[AT]
4774 TCCGGA
BsiEI 1517 CGGCCG CG[GA][CT]^CG
4220 CGGCCG
BsmBI 1266 CGTCTC CGTCTC(1/5)
1806 GAGACG
BstEII 1755 GGTGACC G^GT[ACGT]ACC
4406 GGTCACC
BstPI 1755 GGTGACC G^GT[ACGT]ACC
4406 GGTCACC
CelII 251 GCTGAGC GC^T[ACGT]AGC
1477 GCTCAGC
DraIII 2181 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4616 CACTTAGTG
DrdI 4239 GACCCGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4697 GACGCGGGCGTC
EagI 1517 CGGCCG C^GGCCG
4220 CGGCCG
Eam1105I 716 GACCTAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
918 GACCTGGAGTC
EclXI 1517 CGGCCG C^GGCCG
4220 CGGCCG
Eco52I 1517 CGGCCG C^GGCCG
4220 CGGCCG
EcoO65I 1755 GGTGACC G^GT[ACGT]ACC
4406 GGTCACC
HincII 1438 GTTGAC GT[CT]^[GA]AC
3720 GTCAAC
HindII 1438 GTTGAC GT[CT]^[GA]AC
3720 GTCAAC
MluNI 3958 TGGCCA TGG^CCA
4724 TGGCCA
MscI 3958 TGGCCA TGG^CCA
4724 TGGCCA
MseI 1136 TTAA T^TAA
4453 TTAA
NaeI 1519 GCCGGC GCC^GGC
2976 GCCGGC
NcoI 1692 CCATGG C^CATGG
3304 CCATGG
NgoMIV 1519 GCCGGC G^CCGGC
2976 GCCGGC
NspI 2761 GCATGC [GA]CATG^[CT]
4192 GCATGC
PflFI 1950 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4896 GACTTTGTC
PmaCI 1064 CACGTG CAC^GTG
4716 CACGTG
PmlI 1064 CACGTG CAC^GTG
4716 CACGTG
SacI 377 GAGCTC GAGCT^C
4086 GAGCTC
SphI 2761 GCATGC GCATG^C
4192 GCATGC
Tru9I 1136 TTAA T^TAA
4453 TTAA
Tsp509I 796 AATT ^AATT
5208 AATT
Tth111I 1950 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4896 GACTTTGTC
XmnI 2222 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5207 GAATTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbeI
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SfoI SmaI SmlI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XcmI XhoII XmaCI XmaI