##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI ApaI
AscI Asp718I AspEI AspI AviII
BamHI BbeI BbrPI BcgI BclI
BfrI BglI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspHI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DsaI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI FbaI FseI FspI Hin1I
HincII HindII HpaI KasI KpnI
KspI MamI MluI MroI NaeI
NarI NcoI NgoMIV NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 22 CGCG CG^CG
AflIII 3462 ACATGT A^C[GA][CT]GT
Alw44I 2734 GTGCAC G^TGCAC
AocI 2076 CCTGAGG CC^T[ACGT]AGG
Aor51HI 76 AGCGCT AGC^GCT
ApaLI 2734 GTGCAC G^TGCAC
AvrII 1833 CCTAGG C^CTAGG
BciVI 3435 GTATCC GTATCC(6/5)
BlnI 1833 CCTAGG C^CTAGG
BspLU11I 3462 ACATGT A^CATGT
BspMI 380 ACCTGC ACCTGC(4/8)
BstAPI 2253 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 22 CGCG CG^CG
BstXI 3891 CCAAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2076 CCTGAGG CC^T[ACGT]AGG
CfoI 77 GCGC GCG^C
DraIII 3925 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2817 GACAAATAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 76 AGCGCT AGC^GCT
Eco81I 2076 CCTGAGG CC^T[ACGT]AGG
EcoRV 1603 GATATC GAT^ATC
FauI 66 GCGGG CCCGC(4/6)
HaeII 76 AGCGCT [GA]GCGC^[CT]
HhaI 77 GCGC GCG^C
HinP1I 77 GCGC G^CGC
MvnI 22 CGCG CG^CG
NdeI 1219 CATATG CA^TATG
NheI 86 GCTAGC G^CTAGC
PaeR7I 132 CTCGAG C^TCGAG
PflMI 3083 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmaI 208 CCCGGG CCC^GGG
SpeI 2720 ACTAGT A^CTAGT
SphI 3876 GCATGC GCATG^C
Van91I 3083 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 145 CCACCCTGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 132 CTCGAG C^TCGAG
XmaCI 208 CCCGGG C^CCGGG
XmaI 208 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 57 GGATC GGATC(4/5)
2309 GGATC
AlwNI 499 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
3662 CAGTTCCTG
AseI 2281 ATTAAT AT^TAAT
3957 ATTAAT
AsnI 2281 ATTAAT AT^TAAT
3957 ATTAAT
AvaI 132 CTCGAG C^[CT]CG[GA]G
208 CCCGGG
BalI 193 TGGCCA TGG^CCA
3581 TGGCCA
BanI 170 GGCACC G^G[CT][GA]CC
3078 GGTGCC
BbsI 608 GAAGAC GAAGAC(2/6)
1804 GAAGAC
BbvCI 1707 CCTCAGC CCTCAGC(-5/-2)
393 GCTGAGG
BpuAI 608 GAAGAC GAAGAC(2/6)
1804 GAAGAC
BsmBI 834 CGTCTC CGTCTC(1/5)
41 GAGACG
BsoBI 132 CTCGAG C^[CT]CG[GA]G
208 CCCGGG
BsrDI 1228 GCAATG GCAATG(2/0)
2104 GCAATG
EcoT22I 2052 ATGCAT ATGCA^T
3845 ATGCAT
HgaI 231 GACGC GACGC(5/10)
1103 GCGTC
MaeII 180 ACGT A^CGT
1909 ACGT
MfeI 2795 CAATTG C^AATTG
3803 CAATTG
MluNI 193 TGGCCA TGG^CCA
3581 TGGCCA
MscI 193 TGGCCA TGG^CCA
3581 TGGCCA
MspA1I 3283 CAGCTG C[AC]G^C[GT]G
3783 CAGCTG
MunI 2795 CAATTG C^AATTG
3803 CAATTG
NsiI 2052 ATGCAT ATGCA^T
3845 ATGCAT
PshBI 2281 ATTAAT AT^TAAT
3957 ATTAAT
PvuII 3283 CAGCTG CAG^CTG
3783 CAGCTG
SfcI 2315 CTATAG C^T[GA][CT]AG
2938 CTACAG
SspI 2323 AATATT AAT^ATT
4037 AATATT
TfiI 764 GATTC G^A[AT]TC
1576 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
Asp700I AspHI AvaII BanII BbvI
BcnI BfaI BglII BlpI BmyI
BpmI Bpu1102I BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SmlI SspBI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI