##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AspEI BclI BfrI
BglII BsaBI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssHII ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoRV
EcoT22I FbaI FseI HindIII HpaI
KspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI PaeR7I PinAI PmeI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI ScaI SgrAI SnaBI SphI
SspBI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1522 GTATAC GT^[AC][GT]AC
AcyI 1317 GGCGCC G[GA]^CG[CT]C
Aor51HI 3733 AGCGCT AGC^GCT
AseI 357 ATTAAT AT^TAAT
AsnI 357 ATTAAT AT^TAAT
Asp700I 4221 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3057 TGCGCA TGC^GCA
BamHI 730 GGATCC G^GATCC
BbeI 1317 GGCGCC GGCGC^C
BsaHI 1317 GGCGCC G[GA]^CG[CT]C
BsmI 3249 GCATTC GAATGC(1/-1)
BssSI 5203 CACGAG CACGAG(-5/-1)
Bst1107I 1522 GTATAC GTA^TAC
BstBI 1614 TTCGAA TT^CGAA
BstZ17I 1522 GTATAC GTA^TAC
Eco47III 3733 AGCGCT AGC^GCT
FspI 3057 TGCGCA TGC^GCA
Hin1I 1317 GGCGCC G[GA]^CG[CT]C
HincII 1232 GTTGAC GT[CT]^[GA]AC
HindII 1232 GTTGAC GT[CT]^[GA]AC
KasI 1317 GGCGCC G^GCGCC
NarI 1317 GGCGCC GG^CGCC
NspV 1614 TTCGAA TT^CGAA
PacI 354 TTAATTAA TTAAT^TAA
PshAI 109 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 357 ATTAAT AT^TAAT
SbfI 4727 CCTGCAGG CCTGCA^GG
SfoI 1317 GGCGCC GGC^GCC
SfuI 1614 TTCGAA TT^CGAA
SpeI 1057 ACTAGT A^CTAGT
Sse8387I 4727 CCTGCAGG CCTGCA^GG
SspI 516 AATATT AAT^ATT
XbaI 4808 TCTAGA T^CTAGA
XmnI 4221 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 2225 GGTACC G^GTACC
5176 GGTACC
AocI 2371 CCTCAGG CC^T[ACGT]AGG
3716 CCTCAGG
Asp718I 2225 GGTACC G^GTACC
5176 GGTACC
AspI 377 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
560 GACGGAGTC
BbrPI 1966 CACGTG CAC^GTG
5113 CACGTG
BcgI 822 GCAGCCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4022 GCAGTGGCCTCG
BciVI 3884 GGATAC GTATCC(6/5)
5639 GGATAC
BsmBI 558 GAGACG CGTCTC(1/5)
1408 GAGACG
BspLU11I 951 ACATGT A^CATGT
1328 ACATGT
Bsu36I 2371 CCTCAGG CC^T[ACGT]AGG
3716 CCTCAGG
DrdI 841 GACCAAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5405 GACTGGGTGGTC
EarI 2021 CTCTTC CTCTTC(1/4)
2577 GAAGAG
Eco81I 2371 CCTCAGG CC^T[ACGT]AGG
3716 CCTCAGG
KpnI 2225 GGTACC GGTAC^C
5176 GGTACC
Ksp632I 2021 CTCTTC CTCTTC(1/4)
2577 GAAGAG
NaeI 55 GCCGGC GCC^GGC
2698 GCCGGC
NcoI 3818 CCATGG C^CATGG
4793 CCATGG
NgoMIV 55 GCCGGC G^CCGGC
2698 GCCGGC
PflFI 377 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
560 GACGGAGTC
PmaCI 1966 CACGTG CAC^GTG
5113 CACGTG
PmlI 1966 CACGTG CAC^GTG
5113 CACGTG
SexAI 378 ACCAGGT A^CC[AT]GGT
858 ACCTGGT
SfiI 4660 GGCCCCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5020 GGCCGGTGGGGCC
SmaI 2562 CCCGGG CCC^GGG
5563 CCCGGG
TaqI 831 TCGA T^CGA
1615 TCGA
Tth111I 377 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
560 GACGGAGTC
TthHB8I 831 TCGA T^CGA
1615 TCGA
XmaCI 2562 CCCGGG C^CCGGG
5563 CCCGGG
XmaI 2562 CCCGGG C^CCGGG
5563 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AspHI AvaI AvaII
AvrII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII