##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AhdI Aor51HI ApaI AscI AspEI
AspI AviII BbeI BbrPI BbvCI
BcgI BclI BglI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspMI BsrBI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoT22I FbaI FseI
FspI HaeII Hin1I HpaI KasI
KspI MaeII MamI MluI MroI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 4127 ACATGT A^C[GA][CT]GT
AgeI 3378 ACCGGT A^CCGGT
Alw44I 2914 GTGCAC G^TGCAC
AocI 3444 CCTAAGG CC^T[ACGT]AGG
ApaLI 2914 GTGCAC G^TGCAC
AseI 1855 ATTAAT AT^TAAT
AsnI 1855 ATTAAT AT^TAAT
AvrII 2721 CCTAGG C^CTAGG
BamHI 1418 GGATCC G^GATCC
BciVI 2697 GGATAC GTATCC(6/5)
BglII 3099 AGATCT A^GATCT
BlnI 2721 CCTAGG C^CTAGG
BsaWI 3378 ACCGGT [AT]^CCGG[AT]
BseRI 3356 CTCCTC GAGGAG(10/8)
BsgI 228 GTGCAG GTGCAG(16/14)
BsmBI 142 GAGACG CGTCTC(1/5)
BspLU11I 4127 ACATGT A^CATGT
BsrDI 3881 GCAATG GCAATG(2/0)
BsrFI 3378 ACCGGT [GA]^CCGG[CT]
BssHII 153 GCGCGC G^CGCGC
Bsu36I 3444 CCTAAGG CC^T[ACGT]AGG
Cfr10I 3378 ACCGGT [GA]^CCGG[CT]
Eco81I 3444 CCTAAGG CC^T[ACGT]AGG
EcoNI 2721 CCTAGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 814 GATATC GAT^ATC
HgaI 26 GCGTC GACGC(5/10)
PinAI 3378 ACCGGT A^CCGGT
PshAI 1895 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1855 ATTAAT AT^TAAT
PstI 1610 CTGCAG CTGCA^G
SapI 958 GAAGAGC GCTCTTC(1/4)
SphI 556 GCATGC GCATG^C
TaqI 347 TCGA T^CGA
TthHB8I 347 TCGA T^CGA
XcmI 3152 CCAACCAATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 2789 GGTACC G^GTACC
3716 GGTACC
AflII 580 CTTAAG C^TTAAG
3826 CTTAAG
AlwNI 736 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2879 CAGTCCCTG
Asp718I 2789 GGTACC G^GTACC
3716 GGTACC
AspHI 386 GAGCAC G[AT]GC[AT]^C
2914 GTGCAC
BalI 756 TGGCCA TGG^CCA
3968 TGGCCA
BanII 1406 GGGCTC G[GA]GC[CT]^C
3338 GAGCCC
BfrI 580 CTTAAG C^TTAAG
3826 CTTAAG
BlpI 425 GCTGAGC GC^T[ACGT]AGC
859 GCTGAGC
Bpu1102I 425 GCTGAGC GC^T[ACGT]AGC
859 GCTGAGC
BsaI 2051 GAGACC GGTCTC(1/5)
4020 GGTCTC
BsiHKAI 386 GAGCAC G[AT]GC[AT]^C
2914 GTGCAC
BspHI 615 TCATGA T^CATGA
650 TCATGA
BstAPI 1260 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2812 GCACCAGTTGC
CelII 425 GCTGAGC GC^T[ACGT]AGC
859 GCTGAGC
DsaI 804 CCATGG C^C[GA][CT]GG
1650 CCATGG
EaeI 756 TGGCCA [CT]^GGCC[GA]
3968 TGGCCA
EcoRI 1911 GAATTC G^AATTC
3866 GAATTC
FauI 631 GCGGG CCCGC(4/6)
666 GCGGG
HincII 913 GTCAAC GT[CT]^[GA]AC
1215 GTCAAC
HindII 913 GTCAAC GT[CT]^[GA]AC
1215 GTCAAC
HindIII 583 AAGCTT A^AGCTT
2893 AAGCTT
KpnI 2789 GGTACC GGTAC^C
3716 GGTACC
MfeI 2438 CAATTG C^AATTG
2779 CAATTG
MluNI 756 TGGCCA TGG^CCA
3968 TGGCCA
MscI 756 TGGCCA TGG^CCA
3968 TGGCCA
MunI 2438 CAATTG C^AATTG
2779 CAATTG
NcoI 804 CCATGG C^CATGG
1650 CCATGG
NdeI 1802 CATATG CA^TATG
2534 CATATG
PpuMI 1285 GGGTCCC [GA]G^G[AT]CC[CT]
3727 AGGACCT
RcaI 615 TCATGA T^CATGA
650 TCATGA
SfcI 1610 CTGCAG C^T[GA][CT]AG
3035 CTGTAG
SspI 2425 AATATT AAT^ATT
3547 AATATT
SwaI 3512 ATTTAAAT ATTT^AAAT
4241 ATTTAAAT
XbaI 2047 TCTAGA T^CTAGA
2139 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaI AvaII
BanI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SmaI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII XmaCI XmaI XmnI