##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AvaII AviII AvrII BbeI
BbrPI BglI BlnI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BspDI
BspEI BsrBI BssHII BstAPI BstEII
BstPI ClaI CpoI DraII DsaI
EagI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I FseI FspI HaeII
HgaI Hin1I KasI KpnI KspI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NotI NruI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SphI Sse8387I StuI Van91I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 831 GTATAC GT^[AC][GT]AC
AclI 2821 AACGTT AA^CGTT
AflII 671 CTTAAG C^TTAAG
AhdI 3697 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2306 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3697 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 3342 GAGCAC G[AT]GC[AT]^C
BalI 3076 TGGCCA TGG^CCA
BamHI 753 GGATCC G^GATCC
BanI 501 GGCACC G^G[CT][GA]CC
BciVI 3812 GTATCC GTATCC(6/5)
BfrI 671 CTTAAG C^TTAAG
BglII 2095 AGATCT A^GATCT
BlpI 1596 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1596 GCTCAGC GC^T[ACGT]AGC
BsaAI 2917 CACGTA [CT]AC^GT[GA]
BsaBI 1624 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 3342 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1683 TGTACA T^GTACA
BsrFI 653 GCCGGC [GA]^CCGG[CT]
BsrGI 1683 TGTACA T^GTACA
BssSI 586 CTCGTG CACGAG(-5/-1)
Bst1107I 831 GTATAC GTA^TAC
BstBI 3465 TTCGAA TT^CGAA
BstZ17I 831 GTATAC GTA^TAC
CelII 1596 GCTCAGC GC^T[ACGT]AGC
Cfr10I 653 GCCGGC [GA]^CCGG[CT]
Cfr13I 3684 GGCCC G^G[ACGT]CC
DraIII 2074 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1271 GACACCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 3076 TGGCCA [CT]^GGCC[GA]
Eam1105I 3697 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3288 GAATTC G^AATTC
EcoRV 2993 GATATC GAT^ATC
EcoT22I 2905 ATGCAT ATGCA^T
MamI 1624 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3076 TGGCCA TGG^CCA
MscI 3076 TGGCCA TGG^CCA
NaeI 653 GCCGGC GCC^GGC
NgoMIV 653 GCCGGC G^CCGGC
NsiI 2905 ATGCAT ATGCA^T
NspV 3465 TTCGAA TT^CGAA
PacI 2331 TTAATTAA TTAAT^TAA
Psp1406I 2821 AACGTT AA^CGTT
PvuII 2466 CAGCTG CAG^CTG
Sau96I 3684 GGCCC G^G[ACGT]CC
SfuI 3465 TTCGAA TT^CGAA
SspBI 1683 TGTACA T^GTACA
XmnI 2306 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 419 CGCG CG^CG
650 CGCG
AlwNI 3371 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
3526 CAGTGTCTG
AocI 302 CCTTAGG CC^T[ACGT]AGG
1171 CCTCAGG
AseI 848 ATTAAT AT^TAAT
2330 ATTAAT
AsnI 848 ATTAAT AT^TAAT
2330 ATTAAT
AvaI 1032 CCCGGG C^[CT]CG[GA]G
1510 CCCGGG
BcgI 1044 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2717 CGATGGGGCTGC
BclI 1349 TGATCA T^GATCA
1721 TGATCA
BsgI 146 GTGCAG GTGCAG(16/14)
1338 GTGCAG
BsmFI 1420 GGGAC GGGAC(10/14)
1559 GTCCC
BsmI 1805 GCATTC GAATGC(1/-1)
3065 GCATTC
BsoBI 1032 CCCGGG C^[CT]CG[GA]G
1510 CCCGGG
BspHI 595 TCATGA T^CATGA
3089 TCATGA
BstUI 419 CGCG CG^CG
650 CGCG
Bsu36I 302 CCTTAGG CC^T[ACGT]AGG
1171 CCTCAGG
EarI 836 CTCTTC CTCTTC(1/4)
2940 GAAGAG
Eco81I 302 CCTTAGG CC^T[ACGT]AGG
1171 CCTCAGG
FauI 605 CCCGC CCCGC(4/6)
1185 CCCGC
FbaI 1349 TGATCA T^GATCA
1721 TGATCA
HincII 859 GTTAAC GT[CT]^[GA]AC
2924 GTTAAC
HindII 859 GTTAAC GT[CT]^[GA]AC
2924 GTTAAC
HindIII 1726 AAGCTT A^AGCTT
2266 AAGCTT
HpaI 859 GTTAAC GTT^AAC
2924 GTTAAC
Ksp632I 836 CTCTTC CTCTTC(1/4)
2940 GAAGAG
MslI 1550 CACCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2612 CATTGAAATG
MspA1I 2466 CAGCTG C[AC]G^C[GT]G
2788 CAGCGG
MvnI 419 CGCG CG^CG
650 CGCG
NlaIV 501 GGCACC GG[ACGT]^[ACGT]CC
753 GGATCC
PshBI 848 ATTAAT AT^TAAT
2330 ATTAAT
RcaI 595 TCATGA T^CATGA
3089 TCATGA
SmaI 1032 CCCGGG CCC^GGG
1510 CCCGGG
SpeI 827 ACTAGT A^CTAGT
2243 ACTAGT
SwaI 70 ATTTAAAT ATTT^AAAT
3518 ATTTAAAT
XmaCI 1032 CCCGGG C^CCGGG
1510 CCCGGG
XmaI 1032 CCCGGG C^CCGGG
1510 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsiYI
BslI BsmAI BsmBI Bsp1286I BspLU11I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
DdeI DpnII DraI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NdeII NlaIII NspI
PflFI PleI PstI RsaI Sau3AI
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
XhoII