##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       Acc65I      AccII       Aor51HI     ApaI        
AscI        Asp718I     AspI        AvaI        AviII       
AvrII       BanI        BanII       BbeI        BbvCI       
BclI        BglI        BglII       BlnI        BsiEI       
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspHI       BspMI       BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstXI       BstZ17I     ClaI        CpoI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoO65I     EcoRV       FbaI        FseI        FspI        
HaeII       HindIII     KasI        KpnI        KspI        
MluI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        PacI        
PaeR7I      PflFI       PmeI        PshAI       PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SphI        Sse8387I    SspBI       StuI        
Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccIII        384  TCCGGA               T^CCGGA
AclI         3690  AACGTT               AA^CGTT
AcyI         1124  GGCGTC               G[GA]^CG[CT]C
AgeI         1536  ACCGGT               A^CCGGT
Alw44I        920  GTGCAC               G^TGCAC
AlwNI        3558  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         920  GTGCAC               G^TGCAC
BalI         1092  TGGCCA               TGG^CCA
BamHI         158  GGATCC               G^GATCC
BbrPI        3069  CACGTG               CAC^GTG
BcgI         1437  GCAGCAGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          904  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I      904  GCTAAGC              GC^T[ACGT]AGC
BsaBI         643  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1124  GGCGTC               G[GA]^CG[CT]C
BseAI         384  TCCGGA               T^CCGGA
BsgI         1609  CTGCAC               GTGCAG(16/14)
BsmI          900  GAATGC               GAATGC(1/-1)
BspEI         384  TCCGGA               T^CCGGA
BspLU11I     3716  ACATGT               A^CATGT
BstBI        3932  TTCGAA               TT^CGAA
BstYI         158  GGATCC               [GA]^GATC[CT]
CelII         904  GCTAAGC              GC^T[ACGT]AGC
CfoI         2313  GCGC                 GCG^C
DraIII       4063  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1547  CCACGG               C^C[GA][CT]GG
EcoNI        2074  CCTATGTTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I      3624  CCAAGG               C^C[AT][AT]GG
FauI           13  CCCGC                CCCGC(4/6)
HgaI         1125  GCGTC                GACGC(5/10)
HhaI         2313  GCGC                 GCG^C
Hin1I        1124  GGCGTC               G[GA]^CG[CT]C
HinP1I       2313  GCGC                 G^CGC
HpaI         2817  GTTAAC               GTT^AAC
MamI          643  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          158  GGATCC               [GA]^GATC[CT]
MluNI        1092  TGGCCA               TGG^CCA
MroI          384  TCCGGA               T^CCGGA
MscI         1092  TGGCCA               TGG^CCA
NdeI         2117  CATATG               CA^TATG
NspV         3932  TTCGAA               TT^CGAA
PinAI        1536  ACCGGT               A^CCGGT
PmaCI        3069  CACGTG               CAC^GTG
PmlI         3069  CACGTG               CAC^GTG
Psp1406I     3690  AACGTT               AA^CGTT
PstI         1636  CTGCAG               CTGCA^G
ScaI         2897  AGTACT               AGT^ACT
SfuI         3932  TTCGAA               TT^CGAA
StyI         3624  CCAAGG               C^C[AT][AT]GG
SwaI         3186  ATTTAAAT             ATTT^AAAT
TaqI         3933  TCGA                 T^CGA
TthHB8I      3933  TCGA                 T^CGA
XcmI         1179  CCAGGCTTCTAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         158  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccI         2537  GTAGAC               GT^[AC][GT]AC
             3506  GTAGAC              
AflII        2014  CTTAAG               C^TTAAG
             2023  CTTAAG              
AhdI         1296  GACACTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2861  GACATCAAGTC         
AocI          781  CCTAAGG              CC^T[ACGT]AGG
              852  CCTGAGG             
AspEI        1296  GACACTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2861  GACATCAAGTC         
BbsI         2869  GTCTTC               GAAGAC(2/6)
             3871  GTCTTC              
BcnI         1404  CCCGG                CC^[GC]GG
             1460  CCCGG               
BfrI         2014  CTTAAG               C^TTAAG
             2023  CTTAAG              
BpmI         1432  CTCCAG               CTGGAG(16/14)
             1815  CTCCAG              
BpuAI        2869  GTCTTC               GAAGAC(2/6)
             3871  GTCTTC              
BsaI         2833  GAGACC               GGTCTC(1/5)
              224  GGTCTC              
BsaWI         384  TCCGGA               [AT]^CCGG[AT]
             1536  ACCGGT              
BsrFI        1415  ACCGGC               [GA]^CCGG[CT]
             1536  ACCGGT              
Bsu36I        781  CCTAAGG              CC^T[ACGT]AGG
              852  CCTGAGG             
Cfr10I       1415  ACCGGC               [GA]^CCGG[CT]
             1536  ACCGGT              
EaeI         1092  TGGCCA               [CT]^GGCC[GA]
             1462  CGGCCA              
Eam1105I     1296  GACACTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2861  GACATCAAGTC         
Eco81I        781  CCTAAGG              CC^T[ACGT]AGG
              852  CCTGAGG             
EcoRI         955  GAATTC               G^AATTC
             1583  GAATTC              
EcoT22I      2131  ATGCAT               ATGCA^T
             3666  ATGCAT              
MspA1I       1633  CAGCTG               C[AC]G^C[GT]G
             3569  CAGCTG              
NciI         1404  CCCGG                CC^[GC]GG
             1460  CCCGG               
NsiI         2131  ATGCAT               ATGCA^T
             3666  ATGCAT              
PflMI         622  CCAATGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4062  CCACACTGTGG         
PpuMI        2305  GGGTCCT              [GA]G^G[AT]CC[CT]
             3283  AGGTCCT             
PvuII        1633  CAGCTG               CAG^CTG
             3569  CAGCTG              
SapI         1248  GAAGAGC              GCTCTTC(1/4)
             1408  GCTCTTC             
SmlI         2014  CTTAAG               C^T[CT][GA]AG
             2023  CTTAAG              
SnaBI        3247  TACGTA               TAC^GTA
             3454  TACGTA              
Tsp45I        590  GTGAC                ^GT[GC]AC
             2803  GTCAC               
Van91I        622  CCAATGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4062  CCACACTGTGG         
XbaI         1709  TCTAGA               T^CTAGA
             1832  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        ApoI        AseI        AsnI        Asp700I     
AspHI       AvaII       BbvI        BciVI       BfaI        
BmyI        BsaAI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
DraII       EarI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MfeI        MnlI        MseI        MslI        MspI        
MunI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PshBI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SpeI        SspI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XmnI