##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccII       AciI        AclI        
AcyI        AgeI        AhdI        Alw44I      ApaI        
ApaLI       AscI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BbeI        BclI        BglI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaHI       
BsiEI       BsiWI       BsmBI       BspDI       BspHI       
BspMI       BsrBI       BssHII      BssSI       BstAPI      
BstBI       BstEII      BstPI       BstUI       CelII       
ClaI        CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoO65I     FauI        FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MunI        MvnI        
NarI        NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmeI        Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AccIII       1144  TCCGGA               T^CCGGA
AflII         355  CTTAAG               C^TTAAG
AocI         2134  CCTAAGG              CC^T[ACGT]AGG
Aor51HI        95  AGCGCT               AGC^GCT
AspI         3826  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          969  CTCGGG               C^[CT]CG[GA]G
BamHI         831  GGATCC               G^GATCC
BanI           66  GGTGCC               G^G[CT][GA]CC
BanII          74  GGGCTC               G[GA]GC[CT]^C
BbrPI        1444  CACGTG               CAC^GTG
BbvCI        3352  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1826  CGAAAAGTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         866  GGATAC               GTATCC(6/5)
BcnI          207  CCGGG                CC^[GC]GG
BfrI          355  CTTAAG               C^TTAAG
BsaAI        1444  CACGTG               [CT]AC^GT[GA]
BsaI          847  GAGACC               GGTCTC(1/5)
BsaWI        1144  TCCGGA               [AT]^CCGG[AT]
BseAI        1144  TCCGGA               T^CCGGA
BsmI          543  GAATGC               GAATGC(1/-1)
BsoBI         969  CTCGGG               C^[CT]CG[GA]G
Bsp1407I     3037  TGTACA               T^GTACA
BspEI        1144  TCCGGA               T^CCGGA
BsrFI         107  GCCGGC               [GA]^CCGG[CT]
BsrGI        3037  TGTACA               T^GTACA
Bst1107I     3732  GTATAC               GTA^TAC
BstZ17I      3732  GTATAC               GTA^TAC
Bsu36I       2134  CCTAAGG              CC^T[ACGT]AGG
Cfr10I        107  GCCGGC               [GA]^CCGG[CT]
DraIII       2741  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III       95  AGCGCT               AGC^GCT
Eco81I       2134  CCTAAGG              CC^T[ACGT]AGG
EcoNI          51  CCTGTTCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         453  GAATTC               G^AATTC
EcoT22I      2963  ATGCAT               ATGCA^T
HindIII      1541  AAGCTT               A^AGCTT
MaeII        1445  ACGT                 A^CGT
MroI         1144  TCCGGA               T^CCGGA
NaeI          107  GCCGGC               GCC^GGC
NciI          207  CCGGG                CC^[GC]GG
NdeI         2557  CATATG               CA^TATG
NgoMIV        107  GCCGGC               G^CCGGC
NsiI         2963  ATGCAT               ATGCA^T
PflFI        3826  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1444  CACGTG               CAC^GTG
PmlI         1444  CACGTG               CAC^GTG
PpuMI        1330  GGGTCCC              [GA]G^G[AT]CC[CT]
PshAI        2670  GACTTGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI         2902  AGTACT               AGT^ACT
SspBI        3037  TGTACA               T^GTACA
Tth111I      3826  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         2381  CCAGAGTCGGCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AseI         1936  ATTAAT               AT^TAAT
             3686  ATTAAT              
AsnI         1936  ATTAAT               AT^TAAT
             3686  ATTAAT              
AspHI        1393  GAGCAC               G[AT]GC[AT]^C
             1447  GTGCTC              
BalI         2393  TGGCCA               TGG^CCA
             3224  TGGCCA              
BpmI         1921  CTCCAG               CTGGAG(16/14)
              538  CTGGAG              
BseRI          45  CTCCTC               GAGGAG(10/8)
              329  CTCCTC              
BsiHKAI      1393  GAGCAC               G[AT]GC[AT]^C
             1447  GTGCTC              
BsmFI        1332  GTCCC                GGGAC(10/14)
             2422  GTCCC               
BsrDI         458  CATTGC               GCAATG(2/0)
             2474  CATTGC              
DraII        1280  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1330  GGGTCCC             
EaeI         2393  TGGCCA               [CT]^GGCC[GA]
             3224  TGGCCA              
EcoO109I     1280  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1330  GGGTCCC             
EcoRV         940  GATATC               GAT^ATC
             2932  GATATC              
HaeII          95  AGCGCT               [GA]GCGC^[CT]
              110  GGCGCT              
MluNI        2393  TGGCCA               TGG^CCA
             3224  TGGCCA              
MscI         2393  TGGCCA               TGG^CCA
             3224  TGGCCA              
MspA1I        440  CAGCTG               C[AC]G^C[GT]G
             3350  CAGCTG              
NcoI          731  CCATGG               C^CATGG
             2186  CCATGG              
PflMI          57  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              885  CCAAGAATTGG         
PshBI        1936  ATTAAT               AT^TAAT
             3686  ATTAAT              
PvuII         440  CAGCTG               CAG^CTG
             3350  CAGCTG              
SfcI         2653  CTATAG               C^T[GA][CT]AG
             3192  CTGTAG              
Van91I         57  CCAGGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              885  CCAAGAATTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AccI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        AvaII       BbsI        
BbvI        BfaI        BglII       BmyI        BpuAI       
BsaJI       BsgI        BsiYI       BslI        BsmAI       
Bsp1286I    BspLU11I    BsrI        BssKI       BstF5I      
BstNI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DsaI        
EarI        EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII