##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AvrII BclI BlnI
BsaBI BsaWI BseAI BsiEI BsiWI
BspDI BspEI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I FbaI
FseI HindIII KpnI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
SapI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SwaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 91 GTCGAC GT^[AC][GT]AC
Alw44I 2747 GTGCAC G^TGCAC
AocI 3969 CCTGAGG CC^T[ACGT]AGG
ApaLI 2747 GTGCAC G^TGCAC
BalI 2646 TGGCCA TGG^CCA
BbeI 137 GGCGCC GGCGC^C
BbvCI 696 GCTGAGG CCTCAGC(-5/-2)
BcgI 2008 CGATCCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2667 GGATAC GTATCC(6/5)
BglII 1168 AGATCT A^GATCT
BsmBI 149 CGTCTC CGTCTC(1/5)
BssHII 343 GCGCGC G^CGCGC
BstAPI 2347 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3969 CCTGAGG CC^T[ACGT]AGG
CpoI 3386 CGGTCCG CG^G[AT]CCG
DraIII 2888 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3969 CCTGAGG CC^T[ACGT]AGG
EcoRI 2123 GAATTC G^AATTC
EcoRV 1767 GATATC GAT^ATC
EcoT22I 2471 ATGCAT ATGCA^T
HpaI 4735 GTTAAC GTT^AAC
KasI 137 GGCGCC G^GCGCC
KspI 2156 CCGCGG CCGC^GG
MluNI 2646 TGGCCA TGG^CCA
MscI 2646 TGGCCA TGG^CCA
NarI 137 GGCGCC GG^CGCC
NcoI 1667 CCATGG C^CATGG
NruI 1853 TCGCGA TCG^CGA
NsiI 2471 ATGCAT ATGCA^T
RsrII 3386 CGGTCCG CG^G[AT]CCG
SacII 2156 CCGCGG CCGC^GG
SalI 91 GTCGAC G^TCGAC
SbfI 2689 CCTGCAGG CCTGCA^GG
SfoI 137 GGCGCC GGC^GCC
SmaI 2186 CCCGGG CCC^GGG
Sse8387I 2689 CCTGCAGG CCTGCA^GG
SspI 719 AATATT AAT^ATT
StuI 3973 AGGCCT AGG^CCT
XmaCI 2186 CCCGGG C^CCGGG
XmaI 2186 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 137 GGCGCC G[GA]^CG[CT]C
3861 GGCGTC
AflII 2296 CTTAAG C^TTAAG
4079 CTTAAG
Asp700I 1898 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4261 GAACACCTTC
AspI 2720 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3424 GACCCGGTC
BamHI 4243 GGATCC G^GATCC
4606 GGATCC
BfrI 2296 CTTAAG C^TTAAG
4079 CTTAAG
BglI 3376 GCCCACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3618 GCCGAGCTGGC
BsaHI 137 GGCGCC G[GA]^CG[CT]C
3861 GGCGTC
BsaI 3808 GAGACC GGTCTC(1/5)
4321 GGTCTC
BsrBI 321 GAGCGG CCGCTC(-3/-3)
384 GAGCGG
BsrDI 2056 CATTGC GCAATG(2/0)
547 GCAATG
BsrFI 199 ACCGGC [GA]^CCGG[CT]
1528 ACCGGC
BstXI 1469 CCAGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4328 CCAGTTTCCTGG
Cfr10I 199 ACCGGC [GA]^CCGG[CT]
1528 ACCGGC
EarI 4343 CTCTTC CTCTTC(1/4)
1863 GAAGAG
EcoNI 3849 CCTCTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3953 CCTGTGGGAGG
HgaI 2207 GACGC GACGC(5/10)
3862 GCGTC
Hin1I 137 GGCGCC G[GA]^CG[CT]C
3861 GGCGTC
Ksp632I 4343 CTCTTC CTCTTC(1/4)
1863 GAAGAG
PaeR7I 975 CTCGAG C^TCGAG
3674 CTCGAG
PflFI 2720 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3424 GACCCGGTC
PshAI 1308 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4552 GACCTTTGTC
Tth111I 2720 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3424 GACCCGGTC
XbaI 1200 TCTAGA T^CTAGA
1440 TCTAGA
XcmI 9 CCAGTTTCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
592 CCATTGTGACCCTGG
XhoI 975 CTCGAG C^TCGAG
3674 CTCGAG
XmnI 1898 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4261 GAACACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII AviII BanI
BanII BbrPI BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrGI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII