##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccI AccII AccIII AflII
AgeI Aor51HI ApaI AscI Asp700I
AviII BamHI BbeI BbrPI BcgI
BclI BfrI BglI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BssHII Bst1107I BstBI BstUI
BstZ17I ClaI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KspI MamI MluI
MroI MvnI NarI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PvuI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 4318 AACGTT AA^CGTT
AcyI 3854 GGCGTC G[GA]^CG[CT]C
Alw44I 5538 GTGCAC G^TGCAC
ApaLI 5538 GTGCAC G^TGCAC
AspI 5621 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 4248 GGATAC GTATCC(6/5)
BlpI 230 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 230 GCTTAGC GC^T[ACGT]AGC
BsaHI 3854 GGCGTC G[GA]^CG[CT]C
BsmBI 1525 GAGACG CGTCTC(1/5)
Bsp1407I 4832 TGTACA T^GTACA
BspLU11I 3309 ACATGT A^CATGT
BsrFI 4043 GCCGGC [GA]^CCGG[CT]
BsrGI 4832 TGTACA T^GTACA
BssSI 4359 CACGAG CACGAG(-5/-1)
BstEII 2517 GGTCACC G^GT[ACGT]ACC
BstPI 2517 GGTCACC G^GT[ACGT]ACC
CelII 230 GCTTAGC GC^T[ACGT]AGC
Cfr10I 4043 GCCGGC [GA]^CCGG[CT]
CpoI 3547 CGGTCCG CG^G[AT]CCG
EcoO65I 2517 GGTCACC G^GT[ACGT]ACC
HaeII 3114 GGCGCT [GA]GCGC^[CT]
Hin1I 3854 GGCGTC G[GA]^CG[CT]C
HincII 3922 GTTGAC GT[CT]^[GA]AC
HindII 3922 GTTGAC GT[CT]^[GA]AC
NaeI 4043 GCCGGC GCC^GGC
NgoMIV 4043 GCCGGC G^CCGGC
PaeR7I 5087 CTCGAG C^TCGAG
PflFI 5621 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1386 CCATCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 4318 AACGTT AA^CGTT
RsrII 3547 CGGTCCG CG^G[AT]CCG
SapI 2630 GAAGAGC GCTCTTC(1/4)
SexAI 1105 ACCTGGT A^CC[AT]GGT
SspBI 4832 TGTACA T^GTACA
Tth111I 5621 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1386 CCATCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1545 TCTAGA T^CTAGA
XhoI 5087 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 2420 GGTACC G^GTACC
3173 GGTACC
AflIII 303 ACACGT A^C[GA][CT]GT
3309 ACATGT
AhdI 3786 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3925 GACCTAGGGTC
AseI 1370 ATTAAT AT^TAAT
5231 ATTAAT
AsnI 1370 ATTAAT AT^TAAT
5231 ATTAAT
Asp718I 2420 GGTACC G^GTACC
3173 GGTACC
AspEI 3786 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3925 GACCTAGGGTC
AvrII 3927 CCTAGG C^CTAGG
5497 CCTAGG
BbvCI 764 GCTGAGG CCTCAGC(-5/-2)
1144 GCTGAGG
BglII 1930 AGATCT A^GATCT
4643 AGATCT
BlnI 3927 CCTAGG C^CTAGG
5497 CCTAGG
BsaI 1233 GAGACC GGTCTC(1/5)
5619 GAGACC
BspMI 4452 ACCTGC ACCTGC(4/8)
4488 ACCTGC
BsrDI 3880 CATTGC GCAATG(2/0)
4970 CATTGC
BstAPI 1176 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4222 GCAAAGCATGC
BstXI 1073 CCAGCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3465 CCAAGATGCTGG
Eam1105I 3786 GACAGACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3925 GACCTAGGGTC
HgaI 1173 GACGC GACGC(5/10)
3855 GCGTC
KpnI 2420 GGTACC GGTAC^C
3173 GGTACC
MfeI 4148 CAATTG C^AATTG
5042 CAATTG
MunI 4148 CAATTG C^AATTG
5042 CAATTG
NcoI 4020 CCATGG C^CATGG
5051 CCATGG
NdeI 1852 CATATG CA^TATG
4739 CATATG
PshAI 4593 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4737 GACATATGTC
PshBI 1370 ATTAAT AT^TAAT
5231 ATTAAT
PstI 2734 CTGCAG CTGCA^G
3967 CTGCAG
XcmI 1633 CCAGTGAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5081 CCACAGCTCGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BsrBI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RcaI RsaI SacI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmaI
SmlI SphI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI