##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

AccII       AgeI        AhdI        Aor51HI     AscI        
AspEI       AviII       AvrII       BbvCI       BcgI        
BclI        BcnI        BglI        BlnI        BsiEI       
BsiWI       BspDI       BsrFI       BssHII      BstEII      
BstPI       BstUI       Cfr10I      ClaI        CpoI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRV       FbaI        
FseI        FspI        HpaI        KspI        MluI        
MvnI        NaeI        NciI        NgoMIV      NotI        
NruI        PacI        PinAI       PmeI        PvuI        
RsrII       SacII       SbfI        SfiI        SgrAI       
SmaI        SnaBI       Sse8387I    SwaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII        2463  GACGTC               GACGT^C
Acc65I       3874  GGTACC               G^GTACC
AclI          550  AACGTT               AA^CGTT
Alw44I       1689  GTGCAC               G^TGCAC
ApaI         3249  GGGCCC               GGGCC^C
ApaLI        1689  GTGCAC               G^TGCAC
AseI         6950  ATTAAT               AT^TAAT
AsnI         6950  ATTAAT               AT^TAAT
Asp718I      3874  GGTACC               G^GTACC
BamHI        1634  GGATCC               G^GATCC
BciVI        2183  GTATCC               GTATCC(6/5)
BlpI         3692  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3692  GCTGAGC              GC^T[ACGT]AGC
BsaBI        3752  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         2099  GGTCTC               GGTCTC(1/5)
BsrBI        3829  CCGCTC               CCGCTC(-3/-3)
Bst1107I     2215  GTATAC               GTA^TAC
BstZ17I      2215  GTATAC               GTA^TAC
CelII        3692  GCTGAGC              GC^T[ACGT]AGC
DsaI         3114  CCATGG               C^C[GA][CT]GG
FauI         3828  CCCGC                CCCGC(4/6)
KpnI         3874  GGTACC               GGTAC^C
MamI         3752  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI         3114  CCATGG               C^CATGG
NdeI         1398  CATATG               CA^TATG
PflMI         451  CCACTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        6950  ATTAAT               AT^TAAT
Psp1406I      550  AACGTT               AA^CGTT
SalI         1548  GTCGAC               G^TCGAC
SapI         3201  GCTCTTC              GCTCTTC(1/4)
ScaI         3680  AGTACT               AGT^ACT
SphI         1224  GCATGC               GCATG^C
StuI         2521  AGGCCT               AGG^CCT
Van91I        451  CCACTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         4051  TCTAGA               T^CTAGA
XcmI         3678  CCAGTACTCTATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccIII        214  TCCGGA               T^CCGGA
              805  TCCGGA              
AflII        6422  CTTAAG               C^TTAAG
             7120  CTTAAG              
AocI         4625  CCTCAGG              CC^T[ACGT]AGG
             6235  CCTGAGG             
AspI         3499  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4017  GACCTGGTC           
BbeI         4943  GGCGCC               GGCGC^C
             5784  GGCGCC              
BbrPI        5082  CACGTG               CAC^GTG
             6216  CACGTG              
BfrI         6422  CTTAAG               C^TTAAG
             7120  CTTAAG              
BsaAI        5082  CACGTG               [CT]AC^GT[GA]
             6216  CACGTG              
BsaWI         214  TCCGGA               [AT]^CCGG[AT]
              805  TCCGGA              
BseAI         214  TCCGGA               T^CCGGA
              805  TCCGGA              
BspEI         214  TCCGGA               T^CCGGA
              805  TCCGGA              
BspHI        5605  TCATGA               T^CATGA
             6453  TCATGA              
Bsu36I       4625  CCTCAGG              CC^T[ACGT]AGG
             6235  CCTGAGG             
DraIII       1692  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5544  CACGAGGTG           
Eco81I       4625  CCTCAGG              CC^T[ACGT]AGG
             6235  CCTGAGG             
EcoT22I      5437  ATGCAT               ATGCA^T
             6035  ATGCAT              
KasI         4943  GGCGCC               G^GCGCC
             5784  GGCGCC              
MroI          214  TCCGGA               T^CCGGA
              805  TCCGGA              
NarI         4943  GGCGCC               GG^CGCC
             5784  GGCGCC              
NheI          517  GCTAGC               G^CTAGC
             6469  GCTAGC              
NsiI         5437  ATGCAT               ATGCA^T
             6035  ATGCAT              
PflFI        3499  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4017  GACCTGGTC           
PmaCI        5082  CACGTG               CAC^GTG
             6216  CACGTG              
PmlI         5082  CACGTG               CAC^GTG
             6216  CACGTG              
PshAI        2231  GACTGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3623  GACACAGGTC          
PvuII        1953  CAGCTG               CAG^CTG
             5946  CAGCTG              
RcaI         5605  TCATGA               T^CATGA
             6453  TCATGA              
SfoI         4943  GGCGCC               GGC^GCC
             5784  GGCGCC              
Tth111I      3499  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             4017  GACCTGGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  132 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AflIII      AluI        AlwI        AlwNI       ApoI        
Asp700I     AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BfaI        
BglII       BmyI        BpmI        BpuAI       BsaHI       
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspLU11I    BspMI       
BsrDI       BsrGI       BsrI        BssKI       BssSI       
BstAPI      BstBI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       EaeI        EarI        
EcoO109I    EcoRI       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MfeI        MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MunI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        NspV        PaeR7I      PleI        
PpuMI       PstI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SexAI       SfaNI       SfcI        
SfuI        SmlI        SpeI        SspBI       SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoI        
XhoII       XmnI