##################################
# NO CUT ENZYMES ( 31 enzymes) #
##################################
AscI AvrII BbeI BcgI BciVI
BlnI BsiEI BsiWI CpoI EagI
EclXI Eco52I FseI KasI KspI
MluI NarI NdeI NheI NotI
NruI PacI PmeI PvuI RsrII
SacII SalI SfoI SgrAI SnaBI
XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AclI 6670 AACGTT AA^CGTT
AflII 4762 CTTAAG C^TTAAG
AgeI 2129 ACCGGT A^CCGGT
AhdI 374 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4937 AGCGCT AGC^GCT
AspEI 374 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1185 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2623 CACGTG CAC^GTG
BfrI 4762 CTTAAG C^TTAAG
BsaAI 2623 CACGTG [CT]AC^GT[GA]
BspDI 2815 ATCGAT AT^CGAT
BsrDI 653 GCAATG GCAATG(2/0)
BssHII 1213 GCGCGC G^CGCGC
BssSI 3769 CACGAG CACGAG(-5/-1)
Bst1107I 1081 GTATAC GTA^TAC
BstEII 1158 GGTCACC G^GT[ACGT]ACC
BstPI 1158 GGTCACC G^GT[ACGT]ACC
BstZ17I 1081 GTATAC GTA^TAC
ClaI 2815 ATCGAT AT^CGAT
DrdI 3442 GACATGACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 374 GACAAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4937 AGCGCT AGC^GCT
EcoO65I 1158 GGTCACC G^GT[ACGT]ACC
HpaI 93 GTTAAC GTT^AAC
PflFI 1185 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2129 ACCGGT A^CCGGT
PmaCI 2623 CACGTG CAC^GTG
PmlI 2623 CACGTG CAC^GTG
PshAI 1173 GACCTACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6670 AACGTT AA^CGTT
SbfI 5052 CCTGCAGG CCTGCA^GG
SfiI 2496 GGCCCGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 1773 ACTAGT A^CTAGT
Sse8387I 5052 CCTGCAGG CCTGCA^GG
SwaI 3983 ATTTAAAT ATTT^AAAT
Tth111I 1185 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 707 GGTACC G^GTACC
3215 GGTACC
AccI 1081 GTATAC GT^[AC][GT]AC
8814 GTAGAC
AflIII 4535 ACATGT A^C[GA][CT]GT
5465 ACATGT
ApaI 2495 GGGCCC GGGCC^C
2996 GGGCCC
Asp718I 707 GGTACC G^GTACC
3215 GGTACC
AviII 2978 TGCGCA TGC^GCA
3785 TGCGCA
BamHI 56 GGATCC G^GATCC
7303 GGATCC
BclI 1205 TGATCA T^GATCA
5081 TGATCA
BsaBI 1743 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2073 GATTCGAATC
BsaI 1171 GAGACC GGTCTC(1/5)
1948 GAGACC
BsmBI 2767 CGTCTC CGTCTC(1/5)
2068 GAGACG
Bsp1407I 174 TGTACA T^GTACA
8233 TGTACA
BspLU11I 4535 ACATGT A^CATGT
5465 ACATGT
BsrGI 174 TGTACA T^GTACA
8233 TGTACA
BstAPI 1091 GCAACTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4581 GCACTCCCTGC
BstBI 1842 TTCGAA TT^CGAA
2075 TTCGAA
DraIII 7595 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
8013 CACCAGGTG
EcoRV 211 GATATC GAT^ATC
8105 GATATC
EcoT22I 5568 ATGCAT ATGCA^T
8277 ATGCAT
FbaI 1205 TGATCA T^GATCA
5081 TGATCA
FspI 2978 TGCGCA TGC^GCA
3785 TGCGCA
KpnI 707 GGTACC GGTAC^C
3215 GGTACC
MamI 1743 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2073 GATTCGAATC
MfeI 167 CAATTG C^AATTG
6824 CAATTG
MunI 167 CAATTG C^AATTG
6824 CAATTG
NaeI 3847 GCCGGC GCC^GGC
7216 GCCGGC
NgoMIV 3847 GCCGGC G^CCGGC
7216 GCCGGC
NsiI 5568 ATGCAT ATGCA^T
8277 ATGCAT
NspV 1842 TTCGAA TT^CGAA
2075 TTCGAA
PaeR7I 3778 CTCGAG C^TCGAG
4111 CTCGAG
SapI 1735 GAAGAGC GCTCTTC(1/4)
5070 GCTCTTC
ScaI 6178 AGTACT AGT^ACT
8420 AGTACT
SexAI 4466 ACCTGGT A^CC[AT]GGT
8014 ACCAGGT
SfuI 1842 TTCGAA TT^CGAA
2075 TTCGAA
SmaI 8 CCCGGG CCC^GGG
2552 CCCGGG
SspBI 174 TGTACA T^GTACA
8233 TGTACA
XhoI 3778 CTCGAG C^TCGAG
4111 CTCGAG
XmaCI 8 CCCGGG C^CCGGG
2552 CCCGGG
XmaI 8 CCCGGG C^CCGGG
2552 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AatII AccII AccIII AciI AcsI
AcyI AfaI AluI Alw44I AlwI
AlwNI AocI ApaLI ApoI AseI
AsnI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshBI
PstI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmnI