##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AviII BamHI BbrPI
BciVI BclI BfrI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV EcoT22I FbaI FspI
HindIII HpaI KpnI MfeI MluI
MroI MunI NdeI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
SexAI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 729 GACGTC GACGT^C
AlwI 947 GATCC GGATC(4/5)
AocI 2096 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1858 AGCGCT AGC^GCT
AspI 684 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 237 CCTAGG C^CTAGG
BalI 477 TGGCCA TGG^CCA
BbsI 290 GAAGAC GAAGAC(2/6)
BlnI 237 CCTAGG C^CTAGG
BpuAI 290 GAAGAC GAAGAC(2/6)
BsaAI 1266 TACGTG [CT]AC^GT[GA]
BsaBI 1307 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1340 TCCGGT [AT]^CCGG[AT]
BsmI 1958 GAATGC GAATGC(1/-1)
BspLU11I 1030 ACATGT A^CATGT
BsrDI 3155 GCAATG GCAATG(2/0)
BssHII 1022 GCGCGC G^CGCGC
BssSI 3551 CTCGTG CACGAG(-5/-1)
BstAPI 1946 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 166 CCACTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2096 CCTGAGG CC^T[ACGT]AGG
Eco47III 1858 AGCGCT AGC^GCT
Eco81I 2096 CCTGAGG CC^T[ACGT]AGG
FseI 2592 GGCCGGCC GGCCGG^CC
HincII 2183 GTTGAC GT[CT]^[GA]AC
HindII 2183 GTTGAC GT[CT]^[GA]AC
KspI 844 CCGCGG CCGC^GG
MamI 1307 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 477 TGGCCA TGG^CCA
MscI 477 TGGCCA TGG^CCA
MseI 3772 TTAA T^TAA
NcoI 694 CCATGG C^CATGG
NheI 2083 GCTAGC G^CTAGC
PaeR7I 1594 CTCGAG C^TCGAG
PflFI 684 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 929 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 844 CCGCGG CCGC^GG
ScaI 1204 AGTACT AGT^ACT
SfiI 2805 GGCCCACTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1439 GCATGC GCATG^C
Tru9I 3772 TTAA T^TAA
Tth111I 684 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1594 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 9 enzymes) #
###################################
Alw44I 354 GTGCAC G^TGCAC
1832 GTGCAC
ApaLI 354 GTGCAC G^TGCAC
1832 GTGCAC
BbvCI 950 CCTCAGC CCTCAGC(-5/-2)
821 GCTGAGG
BcgI 1151 CGAGCTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1499 CGACAAGATTGC
BglI 858 GCCCCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2806 GCCCACTCGGC
DrdI 522 GACACAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3373 GACGGTGCAGTC
EcoNI 40 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1873 CCTTTGCCAGG
EcoRI 3254 GAATTC G^AATTC
3584 GAATTC
HgaI 855 GACGC GACGC(5/10)
2609 GACGC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI