##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
AocI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BsaAI BsaBI BsaWI BseAI
BsgI BsiWI BspEI BspLU11I BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcsI 1216 AAATTT [GA]^AATT[CT]
AcyI 678 GACGCC G[GA]^CG[CT]C
Alw44I 2262 GTGCAC G^TGCAC
AlwNI 976 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 895 GGGCCC GGGCC^C
ApaLI 2262 GTGCAC G^TGCAC
ApoI 1216 AAATTT [GA]^AATT[CT]
Asp700I 76 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2105 GGATCC G^GATCC
BglII 289 AGATCT A^GATCT
BsaHI 678 GACGCC G[GA]^CG[CT]C
BsmBI 676 GAGACG CGTCTC(1/5)
Bsp1407I 2524 TGTACA T^GTACA
BspDI 1990 ATCGAT AT^CGAT
BsrBI 2188 GAGCGG CCGCTC(-3/-3)
BsrFI 2407 ACCGGC [GA]^CCGG[CT]
BsrGI 2524 TGTACA T^GTACA
BstXI 359 CCATCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2407 ACCGGC [GA]^CCGG[CT]
ClaI 1990 ATCGAT AT^CGAT
DsaI 2279 CCACGG C^C[GA][CT]GG
EaeI 1564 CGGCCA [CT]^GGCC[GA]
EcoNI 551 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1046 AGCGCC [GA]GCGC^[CT]
Hin1I 678 GACGCC G[GA]^CG[CT]C
HincII 1852 GTTGAC GT[CT]^[GA]AC
HindII 1852 GTTGAC GT[CT]^[GA]AC
HindIII 1949 AAGCTT A^AGCTT
NdeI 1492 CATATG CA^TATG
PaeR7I 1605 CTCGAG C^TCGAG
PvuII 1303 CAGCTG CAG^CTG
SapI 520 GAAGAGC GCTCTTC(1/4)
SbfI 345 CCTGCAGG CCTGCA^GG
SfaNI 1113 GATGC GCATC(5/9)
SphI 1320 GCATGC GCATG^C
Sse8387I 345 CCTGCAGG CCTGCA^GG
SspBI 2524 TGTACA T^GTACA
SspI 220 AATATT AAT^ATT
XbaI 1431 TCTAGA T^CTAGA
XcmI 1877 CCAACCTTGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1605 CTCGAG C^TCGAG
XmnI 76 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaII 486 GGACC G^G[AT]CC
623 GGTCC
BbsI 747 GAAGAC GAAGAC(2/6)
2355 GAAGAC
BlpI 1116 GCTTAGC GC^T[ACGT]AGC
1225 GCTGAGC
Bpu1102I 1116 GCTTAGC GC^T[ACGT]AGC
1225 GCTGAGC
BpuAI 747 GAAGAC GAAGAC(2/6)
2355 GAAGAC
BsiEI 2191 CGGTCG CG[GA][CT]^CG
2247 CGACCG
BsmI 592 GAATGC GAATGC(1/-1)
759 GCATTC
BspHI 684 TCATGA T^CATGA
1617 TCATGA
BspMI 612 ACCTGC ACCTGC(4/8)
2088 GCAGGT
BssSI 1657 CACGAG CACGAG(-5/-1)
935 CTCGTG
BstAPI 1179 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2163 GCAGGCCATGC
CelII 1116 GCTTAGC GC^T[ACGT]AGC
1225 GCTGAGC
CfoI 1047 GCGC GCG^C
2234 GCGC
DraI 1513 TTTAAA TTT^AAA
1780 TTTAAA
DraIII 239 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
661 CACTCAGTG
HgaI 678 GACGC GACGC(5/10)
2358 GACGC
HhaI 1047 GCGC GCG^C
2234 GCGC
HinP1I 1047 GCGC G^CGC
2234 GCGC
NspI 1320 GCATGC [GA]CATG^[CT]
2459 GCATGT
PflMI 1437 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2016 CCAAAGAATGG
PpuMI 485 AGGACCT [GA]G^G[AT]CC[CT]
622 GGGTCCC
RcaI 684 TCATGA T^CATGA
1617 TCATGA
SacI 456 GAGCTC GAGCT^C
940 GAGCTC
Van91I 1437 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2016 CCAAAGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI Sau3AI Sau96I ScrFI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI