##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        Alw44I      ApaI        ApaLI       
AscI        Asp718I     AspHI       AviII       AvrII       
BamHI       BanII       BbeI        BbrPI       BfrI        
BglI        BlnI        BsaAI       BsaHI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BspDI       
BspEI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstZ17I     
Cfr10I      ClaI        CpoI        DraIII      DrdI        
EagI        EclXI       Eco52I      EcoO65I     EcoT22I     
FseI        FspI        Hin1I       HpaI        KasI        
KpnI        MluI        MroI        NaeI        NarI        
NcoI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacI        SbfI        ScaI        SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AflIII       2995  ACATGT               A^C[GA][CT]GT
AocI         3392  CCTCAGG              CC^T[ACGT]AGG
Aor51HI        17  AGCGCT               AGC^GCT
AspI         3183  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BciVI        3544  GTATCC               GTATCC(6/5)
BclI         2186  TGATCA               T^GATCA
BcnI         3321  CCCGG                CC^[GC]GG
BglII        1625  AGATCT               A^GATCT
BlpI         1662  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1662  GCTAAGC              GC^T[ACGT]AGC
BsaBI        2712  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1614  GAGACC               GGTCTC(1/5)
BsaWI        2873  TCCGGT               [AT]^CCGG[AT]
BsgI         1575  GTGCAG               GTGCAG(16/14)
BspLU11I     2995  ACATGT               A^CATGT
Bsu36I       3392  CCTCAGG              CC^T[ACGT]AGG
CelII        1662  GCTAAGC              GC^T[ACGT]AGC
Eco47III       17  AGCGCT               AGC^GCT
Eco81I       3392  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1437  CCTAAATCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         936  GAATTC               G^AATTC
FbaI         2186  TGATCA               T^GATCA
HgaI           47  GACGC                GACGC(5/10)
HindIII      2233  AAGCTT               A^AGCTT
KspI          139  CCGCGG               CCGC^GG
MamI         2712  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3490  CAATTG               C^AATTG
MunI         3490  CAATTG               C^AATTG
NciI         3321  CCCGG                CC^[GC]GG
PflFI        3183  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacII         139  CCGCGG               CCGC^GG
SalI         1290  GTCGAC               G^TCGAC
SapI         2255  GAAGAGC              GCTCTTC(1/4)
Tth111I      3183  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1122  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AccI         1290  GTCGAC               GT^[AC][GT]AC
             1376  GTCTAC              
AccII          49  CGCG                 CG^CG
              140  CGCG                
AhdI         1593  GACAGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2518  GACCAGCTGTC         
AseI         1230  ATTAAT               AT^TAAT
             3926  ATTAAT              
AsnI         1230  ATTAAT               AT^TAAT
             3926  ATTAAT              
AspEI        1593  GACAGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2518  GACCAGCTGTC         
BanI         1466  GGCACC               G^G[CT][GA]CC
             3305  GGCACC              
BcgI         1718  CGAAGCATATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3265  CGATTCTCCTGC        
BmyI         2987  GTGCCC               G[AGT]GC[ACT]^C
             3964  GGGCAC              
Bsp1286I     2987  GTGCCC               G[AGT]GC[ACT]^C
             3964  GGGCAC              
BspHI         965  TCATGA               T^CATGA
             4183  TCATGA              
BsrDI         511  CATTGC               GCAATG(2/0)
             4228  GCAATG              
BstBI         220  TTCGAA               TT^CGAA
             1364  TTCGAA              
BstUI          49  CGCG                 CG^CG
              140  CGCG                
CfoI           18  GCGC                 GCG^C
             3452  GCGC                
DsaI          139  CCGCGG               C^C[GA][CT]GG
              151  CCGTGG              
Eam1105I     1593  GACAGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2518  GACCAGCTGTC         
EcoRV        2291  GATATC               GAT^ATC
             3859  GATATC              
EcoT14I       287  CCTTGG               C^C[AT][AT]GG
              314  CCAAGG              
HaeII          17  AGCGCT               [GA]GCGC^[CT]
             3451  AGCGCC              
HhaI           18  GCGC                 GCG^C
             3452  GCGC                
HinP1I         18  GCGC                 G^CGC
             3452  GCGC                
HincII       1290  GTCGAC               GT[CT]^[GA]AC
             2526  GTCAAC              
HindII       1290  GTCGAC               GT[CT]^[GA]AC
             2526  GTCAAC              
MaeII        1418  ACGT                 A^CGT
             2239  ACGT                
MvnI           49  CGCG                 CG^CG
              140  CGCG                
NspV          220  TTCGAA               TT^CGAA
             1364  TTCGAA              
PaeR7I       1611  CTCGAG               C^TCGAG
             3285  CTCGAG              
PpuMI         134  GGGTCCC              [GA]G^G[AT]CC[CT]
             2726  AGGTCCT             
PshBI        1230  ATTAAT               AT^TAAT
             3926  ATTAAT              
PvuII        2521  CAGCTG               CAG^CTG
             4210  CAGCTG              
RcaI          965  TCATGA               T^CATGA
             4183  TCATGA              
SexAI        2458  ACCTGGT              A^CC[AT]GGT
             3915  ACCTGGT             
SfuI          220  TTCGAA               TT^CGAA
             1364  TTCGAA              
SspI          681  AATATT               AAT^ATT
             3468  AATATT              
StyI          287  CCTTGG               C^C[AT][AT]GG
              314  CCAAGG              
XhoI         1611  CTCGAG               C^TCGAG
             3285  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AvaI        AvaII       
BalI        BbsI        BbvCI       BbvI        BfaI        
BpmI        BpuAI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspMI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        DraII       
EaeI        EarI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NdeI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspBI       TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmnI