##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BcgI BfrI BlnI BlpI
Bpu1102I BsaBI BseAI BsiWI BsmBI
BspDI BspEI BspLU11I Bst1107I BstBI
BstZ17I CelII ClaI CpoI DrdI
Eco47III EcoNI FseI FspI KpnI
KspI MamI MfeI MroI MunI
NdeI NheI NotI NspV PacI
PaeR7I PflFI PmeI PshBI Psp1406I
RsrII SacI SacII SalI SfuI
SgrAI SnaBI StuI SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AgeI 908 ACCGGT A^CCGGT
AhdI 2332 GACACTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 4186 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2332 GACACTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 92 GGCGCC GGCGC^C
BciVI 4365 GTATCC GTATCC(6/5)
BclI 737 TGATCA T^GATCA
BsaI 3248 GGTCTC GGTCTC(1/5)
BsaWI 908 ACCGGT [AT]^CCGG[AT]
Bsp1407I 4629 TGTACA T^GTACA
BsrGI 4629 TGTACA T^GTACA
BssHII 181 GCGCGC G^CGCGC
BstEII 4673 GGTGACC G^GT[ACGT]ACC
BstPI 4673 GGTGACC G^GT[ACGT]ACC
DraI 3364 TTTAAA TTT^AAA
EagI 186 CGGCCG C^GGCCG
Eam1105I 2332 GACACTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 186 CGGCCG C^GGCCG
Eco52I 186 CGGCCG C^GGCCG
EcoO65I 4673 GGTGACC G^GT[ACGT]ACC
EcoRI 3613 GAATTC G^AATTC
EcoRV 2972 GATATC GAT^ATC
EcoT22I 1816 ATGCAT ATGCA^T
FbaI 737 TGATCA T^GATCA
HindIII 605 AAGCTT A^AGCTT
HpaI 1609 GTTAAC GTT^AAC
KasI 92 GGCGCC G^GCGCC
MluI 4540 ACGCGT A^CGCGT
NaeI 4237 GCCGGC GCC^GGC
NarI 92 GGCGCC GG^CGCC
NgoMIV 4237 GCCGGC G^CCGGC
NruI 2808 TCGCGA TCG^CGA
NsiI 1816 ATGCAT ATGCA^T
PinAI 908 ACCGGT A^CCGGT
PshAI 905 GACACCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 47 CGATCG CGAT^CG
SbfI 3158 CCTGCAGG CCTGCA^GG
ScaI 3068 AGTACT AGT^ACT
SexAI 941 ACCTGGT A^CC[AT]GGT
SfiI 106 GGCCAGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 92 GGCGCC GGC^GCC
SmlI 2540 CTCAAG C^T[CT][GA]AG
SpeI 2480 ACTAGT A^CTAGT
Sse8387I 3158 CCTGCAGG CCTGCA^GG
SspBI 4629 TGTACA T^GTACA
SspI 4533 AATATT AAT^ATT
XbaI 2410 TCTAGA T^CTAGA
XmnI 4186 GAAGGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 1126 GACGTC GACGT^C
1720 GACGTC
AflIII 1268 ACGTGT A^C[GA][CT]GT
4540 ACGCGT
AocI 3549 CCTGAGG CC^T[ACGT]AGG
3970 CCTCAGG
ApaI 4663 GGGCCC GGGCC^C
4846 GGGCCC
BbsI 4752 GAAGAC GAAGAC(2/6)
442 GTCTTC
BbvCI 3988 CCTCAGC CCTCAGC(-5/-2)
355 GCTGAGG
BglII 1100 AGATCT A^GATCT
3730 AGATCT
BpuAI 4752 GAAGAC GAAGAC(2/6)
442 GTCTTC
BsiEI 47 CGATCG CG[GA][CT]^CG
186 CGGCCG
BsrBI 2440 CCGCTC CCGCTC(-3/-3)
345 GAGCGG
Bsu36I 3549 CCTGAGG CC^T[ACGT]AGG
3970 CCTCAGG
Eco81I 3549 CCTGAGG CC^T[ACGT]AGG
3970 CCTCAGG
HincII 1609 GTTAAC GT[CT]^[GA]AC
4084 GTTGAC
HindII 1609 GTTAAC GT[CT]^[GA]AC
4084 GTTGAC
PflMI 2767 CCATTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3837 CCACCTCCTGG
PpuMI 1621 GGGACCT [GA]G^G[AT]CC[CT]
1933 GGGACCC
SmaI 96 CCCGGG CCC^GGG
4842 CCCGGG
SphI 480 GCATGC GCATG^C
3896 GCATGC
TaqI 709 TCGA T^CGA
1166 TCGA
TfiI 1307 GATTC G^A[AT]TC
3672 GATTC
TthHB8I 709 TCGA T^CGA
1166 TCGA
Van91I 2767 CCATTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3837 CCACCTCCTGG
XmaCI 96 CCCGGG C^CCGGG
4842 CCCGGG
XmaI 96 CCCGGG C^CCGGG
4842 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbrPI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PmaCI PmlI PstI PvuII RcaI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII