##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AscI AseI AsnI AviII
AvrII BbeI BfrI BlnI BseAI
BsiWI BspDI BspEI BspLU11I BsrFI
BssHII BstUI Cfr10I ClaI CpoI
EcoRI FseI FspI HincII HindII
HpaI KasI KspI MluI MroI
MvnI NaeI NarI NgoMIV NheI
NotI NruI PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SgrAI SnaBI SphI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 3492 GGTACC G^GTACC
AcyI 6830 GACGCC G[GA]^CG[CT]C
AflIII 4435 ACACGT A^C[GA][CT]GT
AhdI 3785 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3447 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2742 AGCGCT AGC^GCT
Asp718I 3492 GGTACC G^GTACC
AspEI 3785 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 4984 TGGCCA TGG^CCA
BbrPI 2735 CACGTG CAC^GTG
BbvCI 452 GCTGAGG CCTCAGC(-5/-2)
BsaAI 2735 CACGTG [CT]AC^GT[GA]
BsaHI 6830 GACGCC G[GA]^CG[CT]C
BsaWI 6200 TCCGGT [AT]^CCGG[AT]
BsiEI 4215 CGGCCG CG[GA][CT]^CG
BssSI 2113 CTCGTG CACGAG(-5/-1)
Bst1107I 122 GTATAC GTA^TAC
BstEII 4878 GGTTACC G^GT[ACGT]ACC
BstPI 4878 GGTTACC G^GT[ACGT]ACC
BstXI 4786 CCACGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 122 GTATAC GTA^TAC
Bsu36I 3447 CCTGAGG CC^T[ACGT]AGG
DraIII 7063 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 4215 CGGCCG C^GGCCG
Eam1105I 3785 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4215 CGGCCG C^GGCCG
Eco47III 2742 AGCGCT AGC^GCT
Eco52I 4215 CGGCCG C^GGCCG
Eco81I 3447 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4878 GGTTACC G^GT[ACGT]ACC
EcoRV 5615 GATATC GAT^ATC
HaeII 2742 AGCGCT [GA]GCGC^[CT]
Hin1I 6830 GACGCC G[GA]^CG[CT]C
KpnI 3492 GGTACC GGTAC^C
MluNI 4984 TGGCCA TGG^CCA
MscI 4984 TGGCCA TGG^CCA
NspI 6939 GCATGT [GA]CATG^[CT]
PaeR7I 2320 CTCGAG C^TCGAG
PmaCI 2735 CACGTG CAC^GTG
PmlI 2735 CACGTG CAC^GTG
PshAI 3672 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 6918 AGTACT AGT^ACT
SmaI 1750 CCCGGG CCC^GGG
SpeI 303 ACTAGT A^CTAGT
XbaI 6480 TCTAGA T^CTAGA
XhoI 2320 CTCGAG C^TCGAG
XmaCI 1750 CCCGGG C^CCGGG
XmaI 1750 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 122 GTATAC GT^[AC][GT]AC
4625 GTCTAC
Alw44I 432 GTGCAC G^TGCAC
4531 GTGCAC
ApaI 3466 GGGCCC GGGCC^C
5495 GGGCCC
ApaLI 432 GTGCAC G^TGCAC
4531 GTGCAC
BcgI 230 GCAACAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1553 GCAAATTGCTCG
BciVI 2913 GTATCC GTATCC(6/5)
4675 GTATCC
BglII 1381 AGATCT A^GATCT
6436 AGATCT
BsmBI 2837 CGTCTC CGTCTC(1/5)
6158 CGTCTC
Bsp1407I 6800 TGTACA T^GTACA
7672 TGTACA
BsrGI 6800 TGTACA T^GTACA
7672 TGTACA
BstBI 238 TTCGAA TT^CGAA
1090 TTCGAA
EcoT22I 2587 ATGCAT ATGCA^T
2991 ATGCAT
HgaI 6830 GACGC GACGC(5/10)
4973 GCGTC
MfeI 1990 CAATTG C^AATTG
2938 CAATTG
MunI 1990 CAATTG C^AATTG
2938 CAATTG
NcoI 5113 CCATGG C^CATGG
6343 CCATGG
NdeI 3754 CATATG CA^TATG
4102 CATATG
NsiI 2587 ATGCAT ATGCA^T
2991 ATGCAT
NspV 238 TTCGAA TT^CGAA
1090 TTCGAA
SapI 1857 GAAGAGC GCTCTTC(1/4)
2118 GAAGAGC
SfuI 238 TTCGAA TT^CGAA
1090 TTCGAA
SspBI 6800 TGTACA T^GTACA
7672 TGTACA
SspI 3762 AATATT AAT^ATT
7770 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AspI
AvaI AvaII BamHI BanI BanII
BbsI BbvI BclI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflFI PflMI PleI
PpuMI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII XmnI