##################################
# NO CUT ENZYMES (   69 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AflII       
AgeI        AhdI        AscI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BbrPI       BbvCI       
BclI        BfrI        BglII       BlnI        BseAI       
BsiEI       BspDI       BspEI       BspHI       BssHII      
Bst1107I    BstAPI      BstBI       BstZ17I     ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco52I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KpnI        MluI        
MroI        NdeI        NotI        NsiI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PvuI        RcaI        RsrII       
SalI        SfuI        SgrAI       SpeI        SspI        
SwaI        Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AclI         1539  AACGTT               AA^CGTT
Alw44I       1986  GTGCAC               G^TGCAC
AocI         4024  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1986  GTGCAC               G^TGCAC
AseI         2083  ATTAAT               AT^TAAT
AsnI         2083  ATTAAT               AT^TAAT
AviII         671  TGCGCA               TGC^GCA
BamHI        3783  GGATCC               G^GATCC
BbeI         1630  GGCGCC               GGCGC^C
BsiWI        1388  CGTACG               C^GTACG
BspLU11I     1796  ACATGT               A^CATGT
BsrDI        3446  CATTGC               GCAATG(2/0)
Bsu36I       4024  CCTGAGG              CC^T[ACGT]AGG
DraIII       1900  CACGAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       4024  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1974  CCTTGCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI          671  TGCGCA               TGC^GCA
KasI         1630  GGCGCC               G^GCGCC
MfeI          624  CAATTG               C^AATTG
MunI          624  CAATTG               C^AATTG
NarI         1630  GGCGCC               GG^CGCC
NcoI         1190  CCATGG               C^CATGG
NheI         3135  GCTAGC               G^CTAGC
NruI         4153  TCGCGA               TCG^CGA
PshBI        2083  ATTAAT               AT^TAAT
Psp1406I     1539  AACGTT               AA^CGTT
SapI         2178  GAAGAGC              GCTCTTC(1/4)
SbfI          609  CCTGCAGG             CCTGCA^GG
ScaI         1841  AGTACT               AGT^ACT
SexAI        2381  ACCTGGT              A^CC[AT]GGT
SfiI         2547  GGCCTTCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1630  GGCGCC               GGC^GCC
SnaBI        2161  TACGTA               TAC^GTA
SphI         2591  GCATGC               GCATG^C
Sse8387I      609  CCTGCAGG             CCTGCA^GG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AcsI         3596  AAATTT               [GA]^AATT[CT]
             3995  AAATTC              
ApoI         3596  AAATTT               [GA]^AATT[CT]
             3995  AAATTC              
BcgI         1326  CGAGGGCCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2507  GCAGGAGGATCG        
BsaBI        2514  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2784  GATTGCCATC          
BsaI         2740  GAGACC               GGTCTC(1/5)
              717  GGTCTC              
BsmI           77  GAATGC               GAATGC(1/-1)
             1877  GCATTC              
Bsp1407I     1799  TGTACA               T^GTACA
             2420  TGTACA              
BsrBI        1105  GAGCGG               CCGCTC(-3/-3)
             1810  GAGCGG              
BsrGI        1799  TGTACA               T^GTACA
             2420  TGTACA              
BstXI         244  CCAAACCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1526  CCACCACAGTGG        
DrdI         2110  GACCAGTACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2380  GACCTGGTGGTC        
EcoT14I      1190  CCATGG               C^C[AT][AT]GG
             1979  CCAAGG              
HincII         62  GTCAAC               GT[CT]^[GA]AC
              790  GTCAAC              
HindII         62  GTCAAC               GT[CT]^[GA]AC
              790  GTCAAC              
KspI         1211  CCGCGG               CCGC^GG
             2907  CCGCGG              
MamI         2514  GATCGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2784  GATTGCCATC          
NaeI         1617  GCCGGC               GCC^GGC
             4118  GCCGGC              
NgoMIV       1617  GCCGGC               G^CCGGC
             4118  GCCGGC              
NspI         1796  ACATGT               [GA]CATG^[CT]
             2591  GCATGC              
SacI          868  GAGCTC               GAGCT^C
             3325  GAGCTC              
SacII        1211  CCGCGG               CCGC^GG
             2907  CCGCGG              
SmaI         2647  CCCGGG               CCC^GGG
             3399  CCCGGG              
SspBI        1799  TGTACA               T^GTACA
             2420  TGTACA              
StyI         1190  CCATGG               C^C[AT][AT]GG
             1979  CCAAGG              
XmaCI        2647  CCCGGG               C^CCGGG
             3399  CCCGGG              
XmaI         2647  CCCGGG               C^CCGGG
             3399  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  128 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       Aor51HI     ApaI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbsI        BbvI        BciVI       BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaHI       BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BssSI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        Eco47III    EcoO109I    
EcoO65I     EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PaeR7I      PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StuI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XcmI        XhoI        XhoII