##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AscI AseI AsnI Asp718I
AvrII BamHI BbeI BcgI BciVI
BfrI BglII BlnI BsaBI BsiEI
BsiWI BspDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoO65I EcoRV
EcoT22I FseI KasI KpnI KspI
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 308 CGCG CG^CG
AccIII 1386 TCCGGA T^CCGGA
AcyI 559 GACGCC G[GA]^CG[CT]C
AhdI 225 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1856 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 225 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1231 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1027 TGCGCA TGC^GCA
BbrPI 2397 CACGTG CAC^GTG
BclI 2684 TGATCA T^GATCA
BlpI 199 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 199 GCTGAGC GC^T[ACGT]AGC
BsaAI 2397 CACGTG [CT]AC^GT[GA]
BsaHI 559 GACGCC G[GA]^CG[CT]C
BseAI 1386 TCCGGA T^CCGGA
BsmI 731 GCATTC GAATGC(1/-1)
BspEI 1386 TCCGGA T^CCGGA
BspHI 3976 TCATGA T^CATGA
BspLU11I 2774 ACATGT A^CATGT
BsrBI 3588 CCGCTC CCGCTC(-3/-3)
BsrFI 382 ACCGGC [GA]^CCGG[CT]
BssSI 1716 CTCGTG CACGAG(-5/-1)
BstUI 308 CGCG CG^CG
CelII 199 GCTGAGC GC^T[ACGT]AGC
Cfr10I 382 ACCGGC [GA]^CCGG[CT]
DraI 207 TTTAAA TTT^AAA
DraIII 698 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 225 GACTTAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2684 TGATCA T^GATCA
FspI 1027 TGCGCA TGC^GCA
Hin1I 559 GACGCC G[GA]^CG[CT]C
HincII 3273 GTTAAC GT[CT]^[GA]AC
HindII 3273 GTTAAC GT[CT]^[GA]AC
HpaI 3273 GTTAAC GTT^AAC
MroI 1386 TCCGGA T^CCGGA
MvnI 308 CGCG CG^CG
PflFI 1231 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2397 CACGTG CAC^GTG
PmlI 2397 CACGTG CAC^GTG
RcaI 3976 TCATGA T^CATGA
Tth111I 1231 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1856 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 976 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
988 CAGCCTCTG
AocI 1760 CCTCAGG CC^T[ACGT]AGG
4286 CCTTAGG
Aor51HI 2317 AGCGCT AGC^GCT
2368 AGCGCT
ApaI 959 GGGCCC GGGCC^C
3352 GGGCCC
BalI 1793 TGGCCA TGG^CCA
4183 TGGCCA
BglI 449 GCCTGACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1035 GCCTCCGAGGC
BsmBI 1130 GAGACG CGTCTC(1/5)
2665 GAGACG
BsrDI 2645 GCAATG GCAATG(2/0)
3877 GCAATG
Bsu36I 1760 CCTCAGG CC^T[ACGT]AGG
4286 CCTTAGG
EaeI 1793 TGGCCA [CT]^GGCC[GA]
4183 TGGCCA
Eco47III 2317 AGCGCT AGC^GCT
2368 AGCGCT
Eco81I 1760 CCTCAGG CC^T[ACGT]AGG
4286 CCTTAGG
EcoNI 1161 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1934 CCTTTATGAGG
FauI 1435 CCCGC CCCGC(4/6)
4385 CCCGC
HindIII 1662 AAGCTT A^AGCTT
3739 AAGCTT
MluNI 1793 TGGCCA TGG^CCA
4183 TGGCCA
MscI 1793 TGGCCA TGG^CCA
4183 TGGCCA
PaeR7I 517 CTCGAG C^TCGAG
934 CTCGAG
SacI 1052 GAGCTC GAGCT^C
4161 GAGCTC
XhoI 517 CTCGAG C^TCGAG
934 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI ApaLI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DrdI DsaI EarI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI SphI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI