##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AccII       AclI        AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       AscI        AspEI       AspI        
AvaI        AviII       AvrII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsiEI       
BsiWI       BsoBI       BspDI       BsrBI       BsrFI       
BssHII      BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       CelII       CfoI        Cfr10I      
ClaI        CpoI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoT22I     
FauI        FseI        FspI        HaeII       HgaI        
HhaI        HinP1I      HpaI        KasI        KspI        
MluI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SphI        Sse8387I    SspI        TaqI        Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII        1350  GACGTC               GACGT^C
Acc65I       4288  GGTACC               G^GTACC
AccIII       2018  TCCGGA               T^CCGGA
AcyI         1350  GACGTC               G[GA]^CG[CT]C
AflII        3515  CTTAAG               C^TTAAG
AflIII       3435  ACATGT               A^C[GA][CT]GT
AlwNI        3686  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1611  CCTCAGG              CC^T[ACGT]AGG
ApaI         2713  GGGCCC               GGGCC^C
Asp718I      4288  GGTACC               G^GTACC
BbsI         2331  GAAGAC               GAAGAC(2/6)
BcnI         1934  CCCGG                CC^[GC]GG
BfrI         3515  CTTAAG               C^TTAAG
BpuAI        2331  GAAGAC               GAAGAC(2/6)
BsaBI        2659  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1350  GACGTC               G[GA]^CG[CT]C
BsaWI        2018  TCCGGA               [AT]^CCGG[AT]
BseAI        2018  TCCGGA               T^CCGGA
BsmBI         435  CGTCTC               CGTCTC(1/5)
BspEI        2018  TCCGGA               T^CCGGA
BspHI        1326  TCATGA               T^CATGA
BspLU11I     3435  ACATGT               A^CATGT
BssSI        4750  CACGAG               CACGAG(-5/-1)
Bsu36I       1611  CCTCAGG              CC^T[ACGT]AGG
DraIII       5079  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       1611  CCTCAGG              CC^T[ACGT]AGG
EcoNI        3635  CCTGATACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I        1350  GACGTC               G[GA]^CG[CT]C
KpnI         4288  GGTACC               GGTAC^C
MamI         2659  GATGTATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2309  CAATTG               C^AATTG
MroI         2018  TCCGGA               T^CCGGA
MunI         2309  CAATTG               C^AATTG
NciI         1934  CCCGG                CC^[GC]GG
NcoI         2933  CCATGG               C^CATGG
PpuMI        4439  AGGACCC              [GA]G^G[AT]CC[CT]
RcaI         1326  TCATGA               T^CATGA
SexAI        4291  ACCAGGT              A^CC[AT]GGT
SpeI         3906  ACTAGT               A^CTAGT
StuI         2489  AGGCCT               AGG^CCT
SwaI         1986  ATTTAAAT             ATTT^AAAT


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AseI          760  ATTAAT               AT^TAAT
             4055  ATTAAT              
AsnI          760  ATTAAT               AT^TAAT
             4055  ATTAAT              
AspHI         173  GAGCAC               G[AT]GC[AT]^C
             4747  GAGCAC              
BsaI           71  GAGACC               GGTCTC(1/5)
             5141  GGTCTC              
BsgI         2998  CTGCAC               GTGCAG(16/14)
             3694  GTGCAG              
BsiHKAI       173  GAGCAC               G[AT]GC[AT]^C
             4747  GAGCAC              
BspMI        1112  ACCTGC               ACCTGC(4/8)
              122  GCAGGT              
BsrDI        2162  GCAATG               GCAATG(2/0)
             3956  GCAATG              
DsaI         2933  CCATGG               C^C[GA][CT]GG
             3352  CCGTGG              
HapII        1935  CCGG                 C^CGG
             2019  CCGG                
HpaII        1935  CCGG                 C^CGG
             2019  CCGG                
MspI         1935  CCGG                 C^CGG
             2019  CCGG                
PshBI         760  ATTAAT               AT^TAAT
             4055  ATTAAT              
ScaI         1531  AGTACT               AGT^ACT
             2477  AGTACT              
SmlI         3515  CTTAAG               C^T[CT][GA]AG
             4936  CTCAAG              
XcmI         1083  CCACAGGAGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5040  CCATCATTTTACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AvaII       BalI        
BamHI       BanI        BanII       BbvI        BclI        
BfaI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    Bsp1407I    BsrGI       BsrI        BssKI       
Bst1107I    BstF5I      BstNI       BstYI       BstZ17I     
Cac8I       Cfr13I      DdeI        DpnII       DraI        
DraII       EaeI        EarI        EcoO109I    EcoRI       
EcoRII      EcoRV       EcoT14I     FbaI        Fnu4HI      
FokI        HaeIII      HincII      HindII      HindIII     
HinfI       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspBI       StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       XhoII       XmnI