##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Aor51HI AscI Asp700I AspEI
BbeI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BspEI BssHII Bst1107I
BstBI BstZ17I DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI Hin1I HpaI KasI KspI
MamI MluI MroI NarI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI Psp1406I PvuI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1258 GGTACC G^GTACC
AccII 3008 CGCG CG^CG
Asp718I 1258 GGTACC G^GTACC
AviII 1085 TGCGCA TGC^GCA
BcgI 6743 GCAGAACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 3864 GCCAGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmI 4560 GCATTC GAATGC(1/-1)
Bsp1407I 3388 TGTACA T^GTACA
BspDI 7894 ATCGAT AT^CGAT
BspLU11I 7455 ACATGT A^CATGT
BsrGI 3388 TGTACA T^GTACA
BstAPI 1308 GCACATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3360 GGTAACC G^GT[ACGT]ACC
BstPI 3360 GGTAACC G^GT[ACGT]ACC
BstUI 3008 CGCG CG^CG
ClaI 7894 ATCGAT AT^CGAT
CpoI 4459 CGGACCG CG^G[AT]CCG
DrdI 657 GACTGAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3360 GGTAACC G^GT[ACGT]ACC
FspI 1085 TGCGCA TGC^GCA
HgaI 4901 GCGTC GACGC(5/10)
KpnI 1258 GGTACC GGTAC^C
MfeI 2530 CAATTG C^AATTG
MunI 2530 CAATTG C^AATTG
MvnI 3008 CGCG CG^CG
NaeI 4427 GCCGGC GCC^GGC
NdeI 6424 CATATG CA^TATG
NgoMIV 4427 GCCGGC G^CCGGC
RsrII 4459 CGGACCG CG^G[AT]CCG
ScaI 7257 AGTACT AGT^ACT
SexAI 1001 ACCTGGT A^CC[AT]GGT
SphI 4166 GCATGC GCATG^C
SspBI 3388 TGTACA T^GTACA
XbaI 5909 TCTAGA T^CTAGA
XcmI 1647 CCATAATAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Alw44I 3564 GTGCAC G^TGCAC
7545 GTGCAC
ApaI 5504 GGGCCC GGGCC^C
5763 GGGCCC
ApaLI 3564 GTGCAC G^TGCAC
7545 GTGCAC
AseI 6154 ATTAAT AT^TAAT
7855 ATTAAT
AsnI 6154 ATTAAT AT^TAAT
7855 ATTAAT
AspI 1000 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4619 GACGGAGTC
AvrII 359 CCTAGG C^CTAGG
5105 CCTAGG
BamHI 3407 GGATCC G^GATCC
4136 GGATCC
BbvCI 4878 CCTCAGC CCTCAGC(-5/-2)
2139 GCTGAGG
BciVI 605 GGATAC GTATCC(6/5)
6442 GGATAC
BclI 2826 TGATCA T^GATCA
8136 TGATCA
BglII 1685 AGATCT A^GATCT
7752 AGATCT
BlnI 359 CCTAGG C^CTAGG
5105 CCTAGG
BlpI 1234 GCTGAGC GC^T[ACGT]AGC
1376 GCTCAGC
Bpu1102I 1234 GCTGAGC GC^T[ACGT]AGC
1376 GCTCAGC
BsmBI 4617 GAGACG CGTCTC(1/5)
4783 GAGACG
BsrFI 4427 GCCGGC [GA]^CCGG[CT]
7806 GCCGGT
BssSI 2472 CACGAG CACGAG(-5/-1)
1494 CTCGTG
BstXI 411 CCAAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4340 CCACTCAGATGG
CelII 1234 GCTGAGC GC^T[ACGT]AGC
1376 GCTCAGC
Cfr10I 4427 GCCGGC [GA]^CCGG[CT]
7806 GCCGGT
DraIII 2153 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4511 CACAGTGTG
FbaI 2826 TGATCA T^GATCA
8136 TGATCA
PflFI 1000 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4619 GACGGAGTC
PshBI 6154 ATTAAT AT^TAAT
7855 ATTAAT
SapI 7655 GAAGAGC GCTCTTC(1/4)
7775 GCTCTTC
SpeI 5189 ACTAGT A^CTAGT
5258 ACTAGT
SspI 3625 AATATT AAT^ATT
7512 AATATT
Tth111I 1000 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4619 GACGGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PaeR7I PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoI XhoII