##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BglI BlnI BpuAI BsaAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I CfoI
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I KasI KpnI
KspI MaeII MluI MluNI MroI
MscI MslI MspA1I MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I StuI Tth111I XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflII 1685 CTTAAG C^TTAAG
AflIII 129 ACATGT A^C[GA][CT]GT
Alw44I 1197 GTGCAC G^TGCAC
AlwNI 507 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1197 GTGCAC G^TGCAC
Asp700I 1028 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 1312 GTATCC GTATCC(6/5)
BclI 96 TGATCA T^GATCA
BcnI 568 CCGGG CC^[GC]GG
BfrI 1685 CTTAAG C^TTAAG
BglII 605 AGATCT A^GATCT
BlpI 934 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 934 GCTTAGC GC^T[ACGT]AGC
BsaBI 2025 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 129 ACATGT A^CATGT
BstEII 1748 GGTCACC G^GT[ACGT]ACC
BstPI 1748 GGTCACC G^GT[ACGT]ACC
CelII 934 GCTTAGC GC^T[ACGT]AGC
DraIII 3139 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3003 GACATGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 497 CGGCCA [CT]^GGCC[GA]
EcoNI 1033 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1748 GGTCACC G^GT[ACGT]ACC
FbaI 96 TGATCA T^GATCA
HapII 568 CCGG C^CGG
HpaI 1447 GTTAAC GTT^AAC
HpaII 568 CCGG C^CGG
MamI 2025 GATATAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2412 CAATTG C^AATTG
MspI 568 CCGG C^CGG
MunI 2412 CAATTG C^AATTG
NciI 568 CCGGG CC^[GC]GG
NdeI 3183 CATATG CA^TATG
PstI 1894 CTGCAG CTGCA^G
SacI 554 GAGCTC GAGCT^C
ScaI 176 AGTACT AGT^ACT
SpeI 3637 ACTAGT A^CTAGT
SphI 930 GCATGC GCATG^C
SspI 2782 AATATT AAT^ATT
SwaI 2484 ATTTAAAT ATTT^AAAT
XmnI 1028 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 23 GCGG CCGC(-3/-1)
790 GCGG
AocI 1529 CCTGAGG CC^T[ACGT]AGG
1615 CCTTAGG
AseI 2070 ATTAAT AT^TAAT
3472 ATTAAT
AsnI 2070 ATTAAT AT^TAAT
3472 ATTAAT
AspHI 554 GAGCTC G[AT]GC[AT]^C
1197 GTGCAC
BanII 32 GGGCTC G[GA]GC[CT]^C
554 GAGCTC
BsaI 2764 GAGACC GGTCTC(1/5)
1544 GGTCTC
BseRI 13 GAGGAG GAGGAG(10/8)
3414 GAGGAG
BsiHKAI 554 GAGCTC G[AT]GC[AT]^C
1197 GTGCAC
BsmI 3727 GAATGC GAATGC(1/-1)
939 GCATTC
BstXI 1600 CCATGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3527 CCATTTAGTTGG
Bsu36I 1529 CCTGAGG CC^T[ACGT]AGG
1615 CCTTAGG
DraI 2188 TTTAAA TTT^AAA
2485 TTTAAA
DraII 1966 GGGGCCT [GA]G^G[ACGT]CC[CT]
2438 AGGCCCT
DsaI 1600 CCATGG C^C[GA][CT]GG
2016 CCATGG
Eco81I 1529 CCTGAGG CC^T[ACGT]AGG
1615 CCTTAGG
EcoO109I 1966 GGGGCCT [GA]G^G[ACGT]CC[CT]
2438 AGGCCCT
EcoRI 218 GAATTC G^AATTC
577 GAATTC
HindIII 1592 AAGCTT A^AGCTT
1901 AAGCTT
MwoI 787 GCTGCGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
930 GCATGCTTAGC
NcoI 1600 CCATGG C^CATGG
2016 CCATGG
PflMI 506 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1316 CCAGTGGGTGG
PshBI 2070 ATTAAT AT^TAAT
3472 ATTAAT
TaqI 943 TCGA T^CGA
1372 TCGA
TthHB8I 943 TCGA T^CGA
1372 TCGA
Van91I 506 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1316 CCAGTGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BmyI BpmI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspHI BspMI BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MvaI
NdeII NlaIII NlaIV NspI PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XbaI XhoII