##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AocI ApoI
AscI AseI AsnI Asp718I AspI
BciVI BclI BfrI BglII BsaWI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspHI Bst1107I BstBI BstZ17I
Bsu36I ClaI DraI Eco81I EcoRI
FbaI FseI HindIII HpaI KpnI
MfeI MroI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI SalI ScaI SfuI
SgrAI SnaBI SpeI StuI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AhdI 4772 GACAAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 954 AGCGCT AGC^GCT
Asp700I 870 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4772 GACAAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2482 CCTAGG C^CTAGG
BamHI 860 GGATCC G^GATCC
BlnI 2482 CCTAGG C^CTAGG
BsaBI 2514 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2200 GAGACC GGTCTC(1/5)
BsiEI 26 CGGCCG CG[GA][CT]^CG
Bsp1407I 4100 TGTACA T^GTACA
BsrGI 4100 TGTACA T^GTACA
BssSI 1927 CTCGTG CACGAG(-5/-1)
BstEII 1011 GGTCACC G^GT[ACGT]ACC
BstPI 1011 GGTCACC G^GT[ACGT]ACC
CpoI 4304 CGGACCG CG^G[AT]CCG
DrdI 700 GACAACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 26 CGGCCG C^GGCCG
Eam1105I 4772 GACAAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 26 CGGCCG C^GGCCG
Eco47III 954 AGCGCT AGC^GCT
Eco52I 26 CGGCCG C^GGCCG
EcoNI 392 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1011 GGTCACC G^GT[ACGT]ACC
EcoRV 1446 GATATC GAT^ATC
HincII 3898 GTCAAC GT[CT]^[GA]AC
HindII 3898 GTCAAC GT[CT]^[GA]AC
MamI 2514 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 368 ACGCGT A^CGCGT
MseI 5121 TTAA T^TAA
RsrII 4304 CGGACCG CG^G[AT]CCG
SacI 289 GAGCTC GAGCT^C
SapI 1683 GCTCTTC GCTCTTC(1/4)
SexAI 704 ACCAGGT A^CC[AT]GGT
SspBI 4100 TGTACA T^GTACA
SspI 5117 AATATT AAT^ATT
Tru9I 5121 TTAA T^TAA
XmnI 870 GAAGGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AviII 186 TGCGCA TGC^GCA
4346 TGCGCA
BbrPI 1272 CACGTG CAC^GTG
4054 CACGTG
BbvCI 2253 GCTGAGG CCTCAGC(-5/-2)
2850 GCTGAGG
BspLU11I 2810 ACATGT A^CATGT
4586 ACATGT
BssHII 349 GCGCGC G^CGCGC
456 GCGCGC
EcoT22I 4332 ATGCAT ATGCA^T
4811 ATGCAT
FspI 186 TGCGCA TGC^GCA
4346 TGCGCA
KspI 135 CCGCGG CCGC^GG
879 CCGCGG
NsiI 4332 ATGCAT ATGCA^T
4811 ATGCAT
PmaCI 1272 CACGTG CAC^GTG
4054 CACGTG
PmlI 1272 CACGTG CAC^GTG
4054 CACGTG
PshAI 2315 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3004 GACTGCAGTC
SacII 135 CCGCGG CCGC^GG
879 CCGCGG
SbfI 1387 CCTGCAGG CCTGCA^GG
3969 CCTGCAGG
SfiI 140 GGCCAGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2227 GGCCTCAGTGGCC
SmlI 670 CTCAAG C^T[CT][GA]AG
2506 CTCAAG
SphI 2666 GCATGC GCATG^C
2783 GCATGC
Sse8387I 1387 CCTGCAGG CCTGCA^GG
3969 CCTGCAGG
Tsp509I 797 AATT ^AATT
2794 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI