##################################
# NO CUT ENZYMES (   45 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AscI        
AseI        AsnI        Asp718I     BglII       BsiWI       
BspDI       BspHI       Bst1107I    BstBI       BstZ17I     
ClaI        EcoRI       EcoRV       EcoT22I     FseI        
HpaI        KpnI        MfeI        MluI        MunI        
NaeI        NgoMIV      NotI        NsiI        NspV        
PacI        PaeR7I      PinAI       PmeI        PshBI       
Psp1406I    PvuI        RcaI        SalI        SfuI        
SgrAI       SnaBI       SpeI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AcsI          732  AAATTT               [GA]^AATT[CT]
AflIII        617  ACATGT               A^C[GA][CT]GT
AhdI         7914  GACATTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1107  GTGCAC               G^TGCAC
AocI         4553  CCTCAGG              CC^T[ACGT]AGG
ApaLI        1107  GTGCAC               G^TGCAC
ApoI          732  AAATTT               [GA]^AATT[CT]
AspEI        7914  GACATTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        7159  CCTAGG               C^CTAGG
BbrPI        3219  CACGTG               CAC^GTG
BciVI        2206  GTATCC               GTATCC(6/5)
BclI         6045  TGATCA               T^GATCA
BlnI         7159  CCTAGG               C^CTAGG
BlpI         1712  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1712  GCTCAGC              GC^T[ACGT]AGC
BsaAI        3219  CACGTG               [CT]AC^GT[GA]
BsiEI         295  CGGCCG               CG[GA][CT]^CG
BspLU11I      617  ACATGT               A^CATGT
BssHII        131  GCGCGC               G^CGCGC
BstEII       5730  GGTGACC              G^GT[ACGT]ACC
BstPI        5730  GGTGACC              G^GT[ACGT]ACC
Bsu36I       4553  CCTCAGG              CC^T[ACGT]AGG
CelII        1712  GCTCAGC              GC^T[ACGT]AGC
CpoI          290  CGGTCCG              CG^G[AT]CCG
EagI          295  CGGCCG               C^GGCCG
Eam1105I     7914  GACATTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         295  CGGCCG               C^GGCCG
Eco52I        295  CGGCCG               C^GGCCG
Eco81I       4553  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      5730  GGTGACC              G^GT[ACGT]ACC
FbaI         6045  TGATCA               T^GATCA
HindIII       704  AAGCTT               A^AGCTT
KspI          141  CCGCGG               CCGC^GG
NdeI          859  CATATG               CA^TATG
NheI         6917  GCTAGC               G^CTAGC
NruI          226  TCGCGA               TCG^CGA
PmaCI        3219  CACGTG               CAC^GTG
PmlI         3219  CACGTG               CAC^GTG
RsrII         290  CGGTCCG              CG^G[AT]CCG
SacII         141  CCGCGG               CCGC^GG
SbfI         2376  CCTGCAGG             CCTGCA^GG
SexAI        4655  ACCAGGT              A^CC[AT]GGT
SfiI          287  GGCCGGTCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI         3464  GCATGC               GCATG^C
Sse8387I     2376  CCTGCAGG             CCTGCA^GG
SspI         5115  AATATT               AAT^ATT
XbaI         5636  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AflII        2646  CTTAAG               C^TTAAG
             7179  CTTAAG              
Aor51HI      4856  AGCGCT               AGC^GCT
             7231  AGCGCT              
Asp700I      1566  GAACCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2562  GAACCAGTTC          
BamHI         476  GGATCC               G^GATCC
             3854  GGATCC              
BcgI         3879  CGACCACATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1118  GCAATGGCATCG        
BfrI         2646  CTTAAG               C^TTAAG
             7179  CTTAAG              
BsaBI        1905  GATTTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             7256  GATTGGAATC          
BsaI         1177  GAGACC               GGTCTC(1/5)
             3831  GGTCTC              
DraI         1933  TTTAAA               TTT^AAA
             5012  TTTAAA              
DrdI         2476  GACACCGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2817  GACCTCCCTGTC        
Eco47III     4856  AGCGCT               AGC^GCT
             7231  AGCGCT              
MamI         1905  GATTTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             7256  GATTGGAATC          
PshAI        2585  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             7346  GACCTGAGTC          
SacI          301  GAGCTC               GAGCT^C
             6746  GAGCTC              
SapI          147  GCTCTTC              GCTCTTC(1/4)
              963  GCTCTTC             
ScaI         1727  AGTACT               AGT^ACT
             5316  AGTACT              
XmnI         1566  GAACCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2562  GAACCAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  149 enzymes) #
#########################################

AccI        AccII       AccIII      AciI        AcyI        
AfaI        AluI        AlwI        AlwNI       ApaI        
AspHI       AspI        AvaI        AvaII       AviII       
BalI        BanI        BanII       BbeI        BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspEI       
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssKI       BssSI       BstAPI      BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MroI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflFI       PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmaI        SmlI        SspBI       StuI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XcmI        XhoII       XmaCI       XmaI