##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AspEI       AviII       
AvrII       BamHI       BbeI        BbrPI       BcgI        
BlnI        BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BspDI       BspEI       BsrBI       BsrFI       
BssHII      Bst1107I    BstBI       BstUI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       FseI        FspI        
HaeII       HgaI        Hin1I       HpaI        KasI        
KspI        MfeI        MluI        MroI        MspA1I      
MunI        MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        PvuII       RsrII       
SacI        SacII       SalI        SbfI        ScaI        
SexAI       SfoI        SfuI        SgrAI       SnaBI       
Sse8387I    SspI        StuI        SwaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I       3737  GGTACC               G^GTACC
AflII        4341  CTTAAG               C^TTAAG
ApaI          288  GGGCCC               GGGCC^C
AseI         2708  ATTAAT               AT^TAAT
AsnI         2708  ATTAAT               AT^TAAT
Asp718I      3737  GGTACC               G^GTACC
AspHI        2580  GTGCTC               G[AT]GC[AT]^C
AspI         2453  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         4112  CCCGGG               C^[CT]CG[GA]G
BanII         288  GGGCCC               G[GA]GC[CT]^C
BfrI         4341  CTTAAG               C^TTAAG
BglI         4087  GCCAGGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         3855  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     3855  GCTTAGC              GC^T[ACGT]AGC
BsaAI         826  CACGTA               [CT]AC^GT[GA]
BsaBI         466  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         3237  GAGACC               GGTCTC(1/5)
BsiHKAI      2580  GTGCTC               G[AT]GC[AT]^C
BsmBI        4065  GAGACG               CGTCTC(1/5)
BsmFI          15  GTCCC                GGGAC(10/14)
BsoBI        4112  CCCGGG               C^[CT]CG[GA]G
BspMI         341  ACCTGC               ACCTGC(4/8)
BssSI        3025  CACGAG               CACGAG(-5/-1)
BstEII       2417  GGTAACC              G^GT[ACGT]ACC
BstPI        2417  GGTAACC              G^GT[ACGT]ACC
CelII        3855  GCTTAGC              GC^T[ACGT]AGC
CfoI         3393  GCGC                 GCG^C
EcoO65I      2417  GGTAACC              G^GT[ACGT]ACC
EcoRV        3504  GATATC               GAT^ATC
HhaI         3393  GCGC                 GCG^C
HinP1I       3393  GCGC                 G^CGC
HindIII      4302  AAGCTT               A^AGCTT
KpnI         3737  GGTACC               GGTAC^C
MamI          466  GATGATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI        2453  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         181  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        2708  ATTAAT               AT^TAAT
SfiI         4086  GGCCAGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         4112  CCCGGG               CCC^GGG
SpeI         2692  ACTAGT               A^CTAGT
Tth111I      2453  GACAACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        181  CCAGGTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3803  TCTAGA               T^CTAGA
XmaCI        4112  CCCGGG               C^CCGGG
XmaI         4112  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

BalI          189  TGGCCA               TGG^CCA
             4085  TGGCCA              
BanI         3737  GGTACC               G^G[CT][GA]CC
             4023  GGCACC              
BbsI         2643  GAAGAC               GAAGAC(2/6)
             1018  GTCTTC              
BbvCI        3994  CCTCAGC              CCTCAGC(-5/-2)
             4129  CCTCAGC             
BciVI         176  GTATCC               GTATCC(6/5)
             4432  GTATCC              
BglII         329  AGATCT               A^GATCT
             4345  AGATCT              
BmyI          288  GGGCCC               G[AGT]GC[ACT]^C
             2580  GTGCTC              
BpuAI        2643  GAAGAC               GAAGAC(2/6)
             1018  GTCTTC              
BsgI         1671  GTGCAG               GTGCAG(16/14)
             1839  GTGCAG              
Bsp1286I      288  GGGCCC               G[AGT]GC[ACT]^C
             2580  GTGCTC              
BstAPI       2681  GCAGAGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3522  GCATACCTTGC         
BstXI        3187  CCAGCACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4139  CCAAAGTGCTGG        
DraI         3100  TTTAAA               TTT^AAA
             4407  TTTAAA              
EaeI          189  TGGCCA               [CT]^GGCC[GA]
             4085  TGGCCA              
EcoT22I       536  ATGCAT               ATGCA^T
             2595  ATGCAT              
HincII        643  GTCAAC               GT[CT]^[GA]AC
             2432  GTCAAC              
HindII        643  GTCAAC               GT[CT]^[GA]AC
             2432  GTCAAC              
MluNI         189  TGGCCA               TGG^CCA
             4085  TGGCCA              
MscI          189  TGGCCA               TGG^CCA
             4085  TGGCCA              
NcoI          409  CCATGG               C^CATGG
             4226  CCATGG              
NsiI          536  ATGCAT               ATGCA^T
             2595  ATGCAT              
PpuMI          27  AGGTCCT              [GA]G^G[AT]CC[CT]
               83  AGGACCT             
PstI          475  CTGCAG               CTGCA^G
              737  CTGCAG              
SmlI         3220  CTTGAG               C^T[CT][GA]AG
             4341  CTTAAG              
SphI         1202  GCATGC               GCATG^C
             1538  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        Asp700I     AvaII       
BbvI        BclI        BcnI        BfaI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmI        Bsp1407I    BspHI       BspLU11I    BsrDI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       DsaI        EarI        EcoO109I    EcoRI       
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NdeI        NdeII       NlaIII      NlaIV       
NspI        PleI        RcaI        RsaI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI