##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       AclI        AflII       AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     BbrPI       BciVI       BfrI        BsaAI       
BsaI        BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BsrGI       BssHII      Bst1107I    BstZ17I     
ClaI        CpoI        DraI        DraIII      EagI        
EclXI       Eco47III    Eco52I      EcoT22I     FseI        
HpaI        KspI        MfeI        MunI        NaeI        
NcoI        NgoMIV      NotI        NruI        NsiI        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SgrAI       SmaI        SnaBI       Sse8387I    
SspBI       StuI        SwaI        XbaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

Acc65I        968  GGTACC               G^GTACC
AccIII       1965  TCCGGA               T^CCGGA
AhdI         2998  GACCCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         3499  CCTCAGG              CC^T[ACGT]AGG
Asp718I       968  GGTACC               G^GTACC
AspEI        2998  GACCCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1803  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         447  TGCGCA               TGC^GCA
BalI         3524  TGGCCA               TGG^CCA
BbeI          147  GGCGCC               GGCGC^C
BbsI         3250  GTCTTC               GAAGAC(2/6)
BcgI         1843  GCATGTGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          753  TGATCA               T^GATCA
BglI         3428  GCCCCTGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1987  AGATCT               A^GATCT
BlpI         1636  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1636  GCTCAGC              GC^T[ACGT]AGC
BpuAI        3250  GTCTTC               GAAGAC(2/6)
BsaBI        1988  GATCTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        1965  TCCGGA               T^CCGGA
BspEI        1965  TCCGGA               T^CCGGA
BspHI        1680  TCATGA               T^CATGA
BspLU11I     2782  ACATGT               A^CATGT
BsrBI        2318  GAGCGG               CCGCTC(-3/-3)
BsrFI        2889  GCCGGT               [GA]^CCGG[CT]
BstAPI        490  GCAGATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         867  TTCGAA               TT^CGAA
Bsu36I       3499  CCTCAGG              CC^T[ACGT]AGG
CelII        1636  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       2889  GCCGGT               [GA]^CCGG[CT]
DsaI         1722  CCACGG               C^C[GA][CT]GG
Eam1105I     2998  GACCCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3499  CCTCAGG              CC^T[ACGT]AGG
EcoRI         870  GAATTC               G^AATTC
EcoRV         719  GATATC               GAT^ATC
FbaI          753  TGATCA               T^GATCA
FspI          447  TGCGCA               TGC^GCA
HincII       2786  GTTGAC               GT[CT]^[GA]AC
HindII       2786  GTTGAC               GT[CT]^[GA]AC
KasI          147  GGCGCC               G^GCGCC
KpnI          968  GGTACC               GGTAC^C
MamI         1988  GATCTCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         3809  ACGCGT               A^CGCGT
MluNI        3524  TGGCCA               TGG^CCA
MroI         1965  TCCGGA               T^CCGGA
MscI         3524  TGGCCA               TGG^CCA
NarI          147  GGCGCC               GG^CGCC
NdeI          948  CATATG               CA^TATG
NheI         1151  GCTAGC               G^CTAGC
NspV          867  TTCGAA               TT^CGAA
PaeR7I        888  CTCGAG               C^TCGAG
PflFI        1803  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         1680  TCATGA               T^CATGA
SapI          118  GCTCTTC              GCTCTTC(1/4)
SfoI          147  GGCGCC               GGC^GCC
SfuI          867  TTCGAA               TT^CGAA
SpeI         1675  ACTAGT               A^CTAGT
SphI          582  GCATGC               GCATG^C
SspI          555  AATATT               AAT^ATT
Tth111I      1803  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          789  CCAACCGATTACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          888  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AccI         1319  GTCTAC               GT^[AC][GT]AC
             1454  GTCTAC              
AcyI          147  GGCGCC               G[GA]^CG[CT]C
              672  GACGCC              
AflIII       2782  ACATGT               A^C[GA][CT]GT
             3809  ACGCGT              
AvrII        3216  CCTAGG               C^CTAGG
             3929  CCTAGG              
BbvCI        2359  CCTCAGC              CCTCAGC(-5/-2)
             2751  CCTCAGC             
BlnI         3216  CCTAGG               C^CTAGG
             3929  CCTAGG              
BsaHI         147  GGCGCC               G[GA]^CG[CT]C
              672  GACGCC              
BsaWI        1491  TCCGGT               [AT]^CCGG[AT]
             1965  TCCGGA              
BsrDI         444  CATTGC               GCAATG(2/0)
              766  CATTGC              
BstEII       1351  GGTGACC              G^GT[ACGT]ACC
             3603  GGTCACC             
BstPI        1351  GGTGACC              G^GT[ACGT]ACC
             3603  GGTCACC             
DrdI         1928  GACCCCTATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3241  GACTGCTCTGTC        
EcoO65I      1351  GGTGACC              G^GT[ACGT]ACC
             3603  GGTCACC             
HaeII         147  GGCGCC               [GA]GCGC^[CT]
             2772  AGCGCC              
Hin1I         147  GGCGCC               G[GA]^CG[CT]C
              672  GACGCC              
HindIII       350  AAGCTT               A^AGCTT
             1960  AAGCTT              
PstI          982  CTGCAG               CTGCA^G
             2246  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApaI        ApoI        AspHI       
AvaI        AvaII       BamHI       BanI        BanII       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrI        BssKI       BssSI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII