##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AflIII      
AgeI        AscI        AseI        AsnI        Asp700I     
BciVI       BclI        BfrI        BsaBI       BseAI       
BsiWI       BsmBI       BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BssHII      Bst1107I    BstAPI      
BstBI       BstZ17I     ClaI        CpoI        DrdI        
EcoRV       EcoT22I     FbaI        HincII      HindII      
HpaI        KspI        MamI        MfeI        MluI        
MroI        MunI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmeI        PshAI       PshBI       Psp1406I    
RcaI        RsrII       SacII       SalI        SfuI        
SgrAI       SpeI        SspI        SwaI        XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

Acc65I        667  GGTACC               G^GTACC
AccI          925  GTCTAC               GT^[AC][GT]AC
AhdI          784  GACCAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         5073  CCTGAGG              CC^T[ACGT]AGG
Asp718I       667  GGTACC               G^GTACC
AspEI         784  GACCAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3938  TGCGCA               TGC^GCA
AvrII        5449  CCTAGG               C^CTAGG
BlnI         5449  CCTAGG               C^CTAGG
BsmI         3463  GCATTC               GAATGC(1/-1)
Bsu36I       5073  CCTGAGG              CC^T[ACGT]AGG
DraI         5904  TTTAAA               TTT^AAA
Eam1105I      784  GACCAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       5073  CCTGAGG              CC^T[ACGT]AGG
EcoRI        1681  GAATTC               G^AATTC
FspI         3938  TGCGCA               TGC^GCA
HindIII      4582  AAGCTT               A^AGCTT
KpnI          667  GGTACC               GGTAC^C
PvuI         3684  CGATCG               CGAT^CG
SacI         3787  GAGCTC               GAGCT^C
SapI         2484  GAAGAGC              GCTCTTC(1/4)
ScaI         2135  AGTACT               AGT^ACT
SnaBI        4066  TACGTA               TAC^GTA
SphI         4487  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcsI         1681  GAATTC               [GA]^AATT[CT]
             1755  GAATTT              
ApoI         1681  GAATTC               [GA]^AATT[CT]
             1755  GAATTT              
AspI         1648  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1840  GACTGTGTC           
BbrPI        2934  CACGTG               CAC^GTG
             2961  CACGTG              
BcgI          299  CGAGCCCCTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             4276  CGACAGCGCTGC        
BglII        2588  AGATCT               A^GATCT
             5240  AGATCT              
Bsp1407I     2207  TGTACA               T^GTACA
             3185  TGTACA              
BsrGI        2207  TGTACA               T^GTACA
             3185  TGTACA              
FseI          412  GGCCGGCC             GGCCGG^CC
              416  GGCCGGCC            
MseI         1154  TTAA                 T^TAA
             5905  TTAA                
PflFI        1648  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1840  GACTGTGTC           
PmaCI        2934  CACGTG               CAC^GTG
             2961  CACGTG              
PmlI         2934  CACGTG               CAC^GTG
             2961  CACGTG              
SbfI         1587  CCTGCAGG             CCTGCA^GG
             2151  CCTGCAGG            
SexAI        1649  ACCTGGT              A^CC[AT]GGT
             5087  ACCTGGT             
Sse8387I     1587  CCTGCAGG             CCTGCA^GG
             2151  CCTGCAGG            
SspBI        2207  TGTACA               T^GTACA
             3185  TGTACA              
Tru9I        1154  TTAA                 T^TAA
             5905  TTAA                
Tth111I      1648  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1840  GACTGTGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  148 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       Aor51HI     ApaI        
ApaLI       AspHI       AvaI        AvaII       BalI        
BamHI       BanI        BanII       BbeI        BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BsaWI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BssSI       BstEII      BstF5I      BstNI       BstPI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EagI        
EarI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO109I    EcoO65I     EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfiI        SfoI        SmaI        SmlI        StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI