##################################
# NO CUT ENZYMES (   54 enzymes) #
##################################

AatII       AclI        AflII       AgeI        Alw44I      
Aor51HI     ApaLI       AscI        BbrPI       BfrI        
BsaBI       BsiWI       BspDI       BsrBI       BsrFI       
BssHII      BstEII      BstPI       Cfr10I      ClaI        
CpoI        DrdI        Eco47III    EcoNI       EcoO65I     
FseI        KspI        MamI        MfeI        MluI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        PacI        PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
SfiI        SgrAI       SnaBI       Sse8387I    


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccIII       2090  TCCGGA               T^CCGGA
AflIII       3739  ACATGT               A^C[GA][CT]GT
AlwNI        4398  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         2267  GGGCCC               GGGCC^C
AseI         3169  ATTAAT               AT^TAAT
AsnI         3169  ATTAAT               AT^TAAT
Asp700I      3521  GAATTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         963  TGCGCA               TGC^GCA
AvrII        3456  CCTAGG               C^CTAGG
BclI         2428  TGATCA               T^GATCA
BglII        4870  AGATCT               A^GATCT
BlnI         3456  CCTAGG               C^CTAGG
BseAI        2090  TCCGGA               T^CCGGA
BsiEI        5295  CGGCCG               CG[GA][CT]^CG
Bsp1407I      682  TGTACA               T^GTACA
BspEI        2090  TCCGGA               T^CCGGA
BspLU11I     3739  ACATGT               A^CATGT
BspMI        2030  ACCTGC               ACCTGC(4/8)
BsrGI         682  TGTACA               T^GTACA
BssSI        6076  CACGAG               CACGAG(-5/-1)
Bst1107I     4855  GTATAC               GTA^TAC
BstBI         173  TTCGAA               TT^CGAA
BstZ17I      4855  GTATAC               GTA^TAC
EagI         5295  CGGCCG               C^GGCCG
EclXI        5295  CGGCCG               C^GGCCG
Eco52I       5295  CGGCCG               C^GGCCG
EcoRI        3853  GAATTC               G^AATTC
FbaI         2428  TGATCA               T^GATCA
FspI          963  TGCGCA               TGC^GCA
HindIII       866  AAGCTT               A^AGCTT
HpaI          498  GTTAAC               GTT^AAC
MroI         2090  TCCGGA               T^CCGGA
NspV          173  TTCGAA               TT^CGAA
PaeR7I       4839  CTCGAG               C^TCGAG
PflMI        1008  CCATGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        3169  ATTAAT               AT^TAAT
SexAI         320  ACCTGGT              A^CC[AT]GGT
SfuI          173  TTCGAA               TT^CGAA
SphI         2776  GCATGC               GCATG^C
SspBI         682  TGTACA               T^GTACA
SspI         3242  AATATT               AAT^ATT
Van91I       1008  CCATGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3766  TCTAGA               T^CTAGA
XhoI         4839  CTCGAG               C^TCGAG
XmnI         3521  GAATTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   45 enzymes) #
###################################

AccII        5063  CGCG                 CG^CG
             6250  CGCG                
AcyI         1860  GGCGCC               G[GA]^CG[CT]C
             6166  GGCGCC              
AhdI          600  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              727  GACCAAAAGTC         
AspEI         600  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              727  GACCAAAAGTC         
AspI          319  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6285  GACTCCGTC           
BalI          467  TGGCCA               TGG^CCA
             3389  TGGCCA              
BamHI        2685  GGATCC               G^GATCC
             5866  GGATCC              
BbeI         1860  GGCGCC               GGCGC^C
             6166  GGCGCC              
BbvCI        1425  GCTGAGG              CCTCAGC(-5/-2)
             6194  GCTGAGG             
BcgI         1560  GCACCTGTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2156  GCACGGTCTTCG        
BlpI         2283  GCTGAGC              GC^T[ACGT]AGC
             2636  GCTAAGC             
Bpu1102I     2283  GCTGAGC              GC^T[ACGT]AGC
             2636  GCTAAGC             
BsaAI         634  TACGTG               [CT]AC^GT[GA]
             2245  TACGTG              
BsaHI        1860  GGCGCC               G[GA]^CG[CT]C
             6166  GGCGCC              
BsaWI         884  ACCGGA               [AT]^CCGG[AT]
             2090  TCCGGA              
BsmBI        6123  CGTCTC               CGTCTC(1/5)
             6290  CGTCTC              
BstAPI       1077  GCAGAAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1351  GCAAATGTTGC         
BstUI        5063  CGCG                 CG^CG
             6250  CGCG                
CelII        2283  GCTGAGC              GC^T[ACGT]AGC
             2636  GCTAAGC             
DraII        1612  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             2267  GGGCCCC             
DraIII       2625  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             5033  CACTCTGTG           
Eam1105I      600  GACCATCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              727  GACCAAAAGTC         
EcoO109I     1612  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             2267  GGGCCCC             
EcoRV        1132  GATATC               GAT^ATC
             4373  GATATC              
EcoT22I      1002  ATGCAT               ATGCA^T
             2445  ATGCAT              
HgaI         1620  GACGC                GACGC(5/10)
             4481  GCGTC               
Hin1I        1860  GGCGCC               G[GA]^CG[CT]C
             6166  GGCGCC              
KasI         1860  GGCGCC               G^GCGCC
             6166  GGCGCC              
MluNI         467  TGGCCA               TGG^CCA
             3389  TGGCCA              
MscI          467  TGGCCA               TGG^CCA
             3389  TGGCCA              
MvnI         5063  CGCG                 CG^CG
             6250  CGCG                
NarI         1860  GGCGCC               GG^CGCC
             6166  GGCGCC              
NsiI         1002  ATGCAT               ATGCA^T
             2445  ATGCAT              
PflFI         319  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6285  GACTCCGTC           
PstI         1602  CTGCAG               CTGCA^G
             5140  CTGCAG              
SacI         2404  GAGCTC               GAGCT^C
             5351  GAGCTC              
SfoI         1860  GGCGCC               GGC^GCC
             6166  GGCGCC              
SmaI         5093  CCCGGG               CCC^GGG
             6218  CCCGGG              
SpeI         1165  ACTAGT               A^CTAGT
             4859  ACTAGT              
StuI         1429  AGGCCT               AGG^CCT
             4910  AGGCCT              
SwaI           15  ATTTAAAT             ATTT^AAAT
             4303  ATTTAAAT            
Tth111I       319  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             6285  GACTCCGTC           
XcmI         3803  CCAGGAGCCCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5602  CCAGCTTCCCTGTGG     
XmaCI        5093  CCCGGG               C^CCGGG
             6218  CCCGGG              
XmaI         5093  CCCGGG               C^CCGGG
             6218  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

Acc65I      AccI        AciI        AcsI        AfaI        
AluI        AlwI        AocI        ApoI        Asp718I     
AspHI       AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BciVI       BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspHI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DsaI        EaeI        EarI        Eco81I      
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        KpnI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PvuII       RcaI        
RsaI        SapI        Sau3AI      Sau96I      ScaI        
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII