##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AccIII AflII AgeI AscI
AseI AsnI AspI AviII BbrPI
BciVI BfrI BseAI BsmI Bsp1407I
BspDI BspEI BspHI BsrGI Bst1107I
BstZ17I ClaI CpoI FseI FspI
HpaI KspI MluI MroI NdeI
NotI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SacII SalI SbfI
SexAI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 2256 GGTACC G^GTACC
AclI 6999 AACGTT AA^CGTT
Alw44I 6540 GTGCAC G^TGCAC
Aor51HI 2986 AGCGCT AGC^GCT
ApaLI 6540 GTGCAC G^TGCAC
Asp718I 2256 GGTACC G^GTACC
BclI 3287 TGATCA T^GATCA
BsiEI 301 CGGCCG CG[GA][CT]^CG
BsiWI 3305 CGTACG C^GTACG
BsrDI 1475 GCAATG GCAATG(2/0)
BssSI 3072 CACGAG CACGAG(-5/-1)
BstBI 2739 TTCGAA TT^CGAA
BstEII 20 GGTCACC G^GT[ACGT]ACC
BstPI 20 GGTCACC G^GT[ACGT]ACC
DraIII 984 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 301 CGGCCG C^GGCCG
EclXI 301 CGGCCG C^GGCCG
Eco47III 2986 AGCGCT AGC^GCT
Eco52I 301 CGGCCG C^GGCCG
EcoO65I 20 GGTCACC G^GT[ACGT]ACC
EcoRV 736 GATATC GAT^ATC
EcoT22I 5097 ATGCAT ATGCA^T
FbaI 3287 TGATCA T^GATCA
HincII 812 GTCAAC GT[CT]^[GA]AC
HindII 812 GTCAAC GT[CT]^[GA]AC
KpnI 2256 GGTACC GGTAC^C
MfeI 6874 CAATTG C^AATTG
MunI 6874 CAATTG C^AATTG
NheI 4374 GCTAGC G^CTAGC
NruI 437 TCGCGA TCG^CGA
NsiI 5097 ATGCAT ATGCA^T
NspV 2739 TTCGAA TT^CGAA
Psp1406I 6999 AACGTT AA^CGTT
ScaI 1179 AGTACT AGT^ACT
SfiI 211 GGCCAGAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2739 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1731 GTCTAC GT^[AC][GT]AC
4540 GTAGAC
AflIII 6291 ACATGT A^C[GA][CT]GT
6934 ACATGT
AhdI 1663 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4821 GACTCCAGGTC
Asp700I 1085 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1415 GAAGCTCTTC
AspEI 1663 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4821 GACTCCAGGTC
AvrII 112 CCTAGG C^CTAGG
5062 CCTAGG
BlnI 112 CCTAGG C^CTAGG
5062 CCTAGG
BsaBI 61 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5247 GATCCACATC
BsaWI 1587 ACCGGA [AT]^CCGG[AT]
3616 ACCGGA
BspLU11I 6291 ACATGT A^CATGT
6934 ACATGT
BssHII 449 GCGCGC G^CGCGC
451 GCGCGC
DraI 5189 TTTAAA TTT^AAA
6957 TTTAAA
DrdI 4844 GACCTCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5931 GACTTATGGGTC
Eam1105I 1663 GACAGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4821 GACTCCAGGTC
EcoRI 1722 GAATTC G^AATTC
6331 GAATTC
HindIII 2352 AAGCTT A^AGCTT
5275 AAGCTT
MamI 61 GATGAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5247 GATCCACATC
NaeI 299 GCCGGC GCC^GGC
3198 GCCGGC
NgoMIV 299 GCCGGC G^CCGGC
3198 GCCGGC
PaeR7I 164 CTCGAG C^TCGAG
1505 CTCGAG
SspI 1236 AATATT AAT^ATT
5170 AATATT
XhoI 164 CTCGAG C^TCGAG
1505 CTCGAG
XmnI 1085 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1415 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BamHI BanI BanII BbeI BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI