##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII Acc65I AclI AscI AseI
AsnI Asp718I BciVI BsaBI BsiEI
BsiWI Bsp1407I BspDI BsrGI BssHII
Bst1107I BstZ17I ClaI CpoI DrdI
EagI EclXI Eco52I EcoRI EcoRV
FseI KpnI MamI MfeI MluI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI PacI PmeI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SexAI SfiI SnaBI
SpeI Sse8387I SspBI SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 305 GTCTAC GT^[AC][GT]AC
AgeI 40 ACCGGT A^CCGGT
Alw44I 2624 GTGCAC G^TGCAC
ApaI 4913 GGGCCC GGGCC^C
ApaLI 2624 GTGCAC G^TGCAC
AspI 2963 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3124 TGCGCA TGC^GCA
BalI 1730 TGGCCA TGG^CCA
BbrPI 3024 CACGTG CAC^GTG
BbsI 3624 GTCTTC GAAGAC(2/6)
BcgI 2529 GCAGCCCTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2874 AGATCT A^GATCT
BpuAI 3624 GTCTTC GAAGAC(2/6)
BsmBI 2452 GAGACG CGTCTC(1/5)
BsmI 5057 GAATGC GAATGC(1/-1)
BsrBI 320 GAGCGG CCGCTC(-3/-3)
BssSI 890 CTCGTG CACGAG(-5/-1)
BstBI 3892 TTCGAA TT^CGAA
BstEII 326 GGTGACC G^GT[ACGT]ACC
BstPI 326 GGTGACC G^GT[ACGT]ACC
BstXI 2469 CCAGCTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoO65I 326 GGTGACC G^GT[ACGT]ACC
EcoT22I 702 ATGCAT ATGCA^T
FspI 3124 TGCGCA TGC^GCA
MluNI 1730 TGGCCA TGG^CCA
MscI 1730 TGGCCA TGG^CCA
NsiI 702 ATGCAT ATGCA^T
NspV 3892 TTCGAA TT^CGAA
PaeR7I 93 CTCGAG C^TCGAG
PflFI 2963 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 714 CCACTAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 40 ACCGGT A^CCGGT
PmaCI 3024 CACGTG CAC^GTG
PmlI 3024 CACGTG CAC^GTG
SfuI 3892 TTCGAA TT^CGAA
SgrAI 3263 CGCCGGTG C[GA]^CCGG[CT]G
StuI 296 AGGCCT AGG^CCT
Tth111I 2963 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 714 CCACTAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1493 TCTAGA T^CTAGA
XhoI 93 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 371 GTAC GT^AC
3166 GTAC
AflII 830 CTTAAG C^TTAAG
4634 CTTAAG
AhdI 634 GACGCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3090 GACAGCCTGTC
AocI 971 CCTAAGG CC^T[ACGT]AGG
1349 CCTTAGG
AspEI 634 GACGCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3090 GACAGCCTGTC
AvrII 3068 CCTAGG C^CTAGG
4605 CCTAGG
BbeI 341 GGCGCC GGCGC^C
3814 GGCGCC
BbvCI 195 CCTCAGC CCTCAGC(-5/-2)
4038 CCTCAGC
BfrI 830 CTTAAG C^TTAAG
4634 CTTAAG
BlnI 3068 CCTAGG C^CTAGG
4605 CCTAGG
BsaAI 372 TACGTG [CT]AC^GT[GA]
3024 CACGTG
BspLU11I 1388 ACATGT A^CATGT
5071 ACATGT
BsrDI 424 CATTGC GCAATG(2/0)
1126 GCAATG
BsrFI 40 ACCGGT [GA]^CCGG[CT]
3264 GCCGGT
Bsu36I 971 CCTAAGG CC^T[ACGT]AGG
1349 CCTTAGG
Cfr10I 40 ACCGGT [GA]^CCGG[CT]
3264 GCCGGT
DraI 956 TTTAAA TTT^AAA
4426 TTTAAA
DraIII 2214 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
3858 CACAATGTG
EaeI 149 CGGCCA [CT]^GGCC[GA]
1730 TGGCCA
Eam1105I 634 GACGCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3090 GACAGCCTGTC
Eco81I 971 CCTAAGG CC^T[ACGT]AGG
1349 CCTTAGG
EcoNI 2442 CCTTCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3704 CCTGCTTCAGG
HpaI 2051 GTTAAC GTT^AAC
4332 GTTAAC
KasI 341 GGCGCC G^GCGCC
3814 GGCGCC
KspI 331 CCGCGG CCGC^GG
4059 CCGCGG
MslI 2699 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3000 CATCCCAGTG
NarI 341 GGCGCC GG^CGCC
3814 GGCGCC
PpuMI 124 GGGTCCC [GA]G^G[AT]CC[CT]
654 GGGACCT
PshAI 119 GACGGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4632 GACTTAAGTC
RsaI 371 GTAC GT^AC
3166 GTAC
SacII 331 CCGCGG CCGC^GG
4059 CCGCGG
SfoI 341 GGCGCC GGC^GCC
3814 GGCGCC
SphI 2209 GCATGC GCATG^C
3505 GCATGC
SspI 4419 AATATT AAT^ATT
4491 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AccIII AciI AcsI AcyI
AflIII AluI AlwI AlwNI Aor51HI
ApoI Asp700I AspHI AvaI AvaII
BamHI BanI BanII BbvI BclI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspHI BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
Eco47III EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MroI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RcaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmaCI XmaI XmnI