##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
Acc65I AflII AflIII AgeI ApaI
AscI Asp718I AviII BbrPI BclI
BfrI BsiWI BspLU11I BssHII Bst1107I
BstEII BstPI BstZ17I CpoI DrdI
EcoO65I FbaI FseI FspI KpnI
MluI NotI NruI PacI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SalI SbfI ScaI SexAI
SgrAI SpeI SphI Sse8387I SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1563 GACGTC GACGT^C
AclI 1625 AACGTT AA^CGTT
AhdI 3334 GACTTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4999 GTGCAC G^TGCAC
Aor51HI 2003 AGCGCT AGC^GCT
ApaLI 4999 GTGCAC G^TGCAC
AspEI 3334 GACTTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1767 CCTAGG C^CTAGG
BamHI 5052 GGATCC G^GATCC
BbeI 1895 GGCGCC GGCGC^C
BcgI 2904 CGAGCGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1767 CCTAGG C^CTAGG
BsaAI 2017 TACGTA [CT]AC^GT[GA]
BspDI 1393 ATCGAT AT^CGAT
BspMI 2349 GCAGGT ACCTGC(4/8)
BstBI 4503 TTCGAA TT^CGAA
ClaI 1393 ATCGAT AT^CGAT
EagI 2945 CGGCCG C^GGCCG
Eam1105I 3334 GACTTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2945 CGGCCG C^GGCCG
Eco47III 2003 AGCGCT AGC^GCT
Eco52I 2945 CGGCCG C^GGCCG
EcoT22I 276 ATGCAT ATGCA^T
HpaI 1804 GTTAAC GTT^AAC
KasI 1895 GGCGCC G^GCGCC
KspI 1199 CCGCGG CCGC^GG
MfeI 5042 CAATTG C^AATTG
MunI 5042 CAATTG C^AATTG
NarI 1895 GGCGCC GG^CGCC
NdeI 4610 CATATG CA^TATG
NheI 1461 GCTAGC G^CTAGC
NsiI 276 ATGCAT ATGCA^T
NspV 4503 TTCGAA TT^CGAA
PaeR7I 251 CTCGAG C^TCGAG
Psp1406I 1625 AACGTT AA^CGTT
SacII 1199 CCGCGG CCGC^GG
SapI 1933 GAAGAGC GCTCTTC(1/4)
SfiI 2439 GGCCCAATGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1895 GGCGCC GGC^GCC
SfuI 4503 TTCGAA TT^CGAA
SmaI 5165 CCCGGG CCC^GGG
SnaBI 2017 TACGTA TAC^GTA
XhoI 251 CTCGAG C^TCGAG
XmaCI 5165 CCCGGG C^CCGGG
XmaI 5165 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1596 GTCTAC GT^[AC][GT]AC
5116 GTAGAC
AccII 1200 CGCG CG^CG
6955 CGCG
AccIII 1949 TCCGGA T^CCGGA
2453 TCCGGA
AocI 4168 CCTGAGG CC^T[ACGT]AGG
6899 CCTGAGG
AseI 1012 ATTAAT AT^TAAT
3448 ATTAAT
AsnI 1012 ATTAAT AT^TAAT
3448 ATTAAT
AspI 3398 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4350 GACCTTGTC
BciVI 5317 GGATAC GTATCC(6/5)
3153 GTATCC
BsaBI 1378 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4501 GATTCGAATC
BsaWI 1949 TCCGGA [AT]^CCGG[AT]
2453 TCCGGA
BseAI 1949 TCCGGA T^CCGGA
2453 TCCGGA
BsmBI 6830 CGTCTC CGTCTC(1/5)
6359 GAGACG
BsmI 3013 GCATTC GAATGC(1/-1)
3646 GCATTC
Bsp1407I 419 TGTACA T^GTACA
3228 TGTACA
BspEI 1949 TCCGGA T^CCGGA
2453 TCCGGA
BspHI 2643 TCATGA T^CATGA
6028 TCATGA
BsrBI 1891 GAGCGG CCGCTC(-3/-3)
1914 GAGCGG
BsrFI 3028 GCCGGC [GA]^CCGG[CT]
4389 GCCGGC
BsrGI 419 TGTACA T^GTACA
3228 TGTACA
BssSI 2120 CACGAG CACGAG(-5/-1)
6783 CACGAG
BstUI 1200 CGCG CG^CG
6955 CGCG
Bsu36I 4168 CCTGAGG CC^T[ACGT]AGG
6899 CCTGAGG
Cfr10I 3028 GCCGGC [GA]^CCGG[CT]
4389 GCCGGC
DraI 1629 TTTAAA TTT^AAA
5895 TTTAAA
DraIII 853 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3172 CACAGGGTG
Eco81I 4168 CCTGAGG CC^T[ACGT]AGG
6899 CCTGAGG
EcoRI 3275 GAATTC G^AATTC
4987 GAATTC
HincII 1804 GTTAAC GT[CT]^[GA]AC
4015 GTTGAC
HindII 1804 GTTAAC GT[CT]^[GA]AC
4015 GTTGAC
HindIII 1681 AAGCTT A^AGCTT
5614 AAGCTT
MamI 1378 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4501 GATTCGAATC
MroI 1949 TCCGGA T^CCGGA
2453 TCCGGA
MvnI 1200 CGCG CG^CG
6955 CGCG
NaeI 3028 GCCGGC GCC^GGC
4389 GCCGGC
NgoMIV 3028 GCCGGC G^CCGGC
4389 GCCGGC
NspI 5865 GCATGT [GA]CATG^[CT]
6870 ACATGC
PflFI 3398 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4350 GACCTTGTC
PshBI 1012 ATTAAT AT^TAAT
3448 ATTAAT
PstI 2372 CTGCAG CTGCA^G
4184 CTGCAG
RcaI 2643 TCATGA T^CATGA
6028 TCATGA
SspBI 419 TGTACA T^GTACA
3228 TGTACA
StuI 2336 AGGCCT AGG^CCT
2653 AGGCCT
Tth111I 3398 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4350 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI