##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AcsI AflII AgeI
AhdI Alw44I ApaLI ApoI AscI
Asp718I AspEI AvrII BbeI BciVI
BfrI BglII BlnI BsiEI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoRI
EcoT22I FseI HpaI KasI KpnI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmeI PshAI PvuI
RsrII SalI SbfI ScaI SfoI
SfuI SnaBI SpeI Sse8387I SspBI
SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 4727 AACGTT AA^CGTT
AlwNI 579 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 4613 AGCGCT AGC^GCT
ApaI 4184 GGGCCC GGGCC^C
AseI 1234 ATTAAT AT^TAAT
AsnI 1234 ATTAAT AT^TAAT
Asp700I 1379 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 4280 TGGCCA TGG^CCA
BamHI 616 GGATCC G^GATCC
BbrPI 2801 CACGTG CAC^GTG
BbvCI 1765 CCTCAGC CCTCAGC(-5/-2)
BglI 4083 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 3519 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 3404 TCATGA T^CATGA
BsrBI 1879 CCGCTC CCGCTC(-3/-3)
BssHII 818 GCGCGC G^CGCGC
BstAPI 2797 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1903 GGTGACC G^GT[ACGT]ACC
BstPI 1903 GGTGACC G^GT[ACGT]ACC
DraIII 2357 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4613 AGCGCT AGC^GCT
EcoO65I 1903 GGTGACC G^GT[ACGT]ACC
EcoRV 623 GATATC GAT^ATC
HincII 932 GTCAAC GT[CT]^[GA]AC
HindII 932 GTCAAC GT[CT]^[GA]AC
HindIII 3764 AAGCTT A^AGCTT
MamI 3519 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4280 TGGCCA TGG^CCA
MscI 4280 TGGCCA TGG^CCA
PaeR7I 2174 CTCGAG C^TCGAG
PmaCI 2801 CACGTG CAC^GTG
PmlI 2801 CACGTG CAC^GTG
PshBI 1234 ATTAAT AT^TAAT
Psp1406I 4727 AACGTT AA^CGTT
RcaI 3404 TCATGA T^CATGA
SapI 251 GAAGAGC GCTCTTC(1/4)
SfiI 4082 GGCCAGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2346 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 309 CCCGGG CCC^GGG
SphI 3642 GCATGC GCATG^C
SspI 4686 AATATT AAT^ATT
XbaI 457 TCTAGA T^CTAGA
XhoI 2174 CTCGAG C^TCGAG
XmaCI 309 CCCGGG C^CCGGG
XmaI 309 CCCGGG C^CCGGG
XmnI 1379 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 2825 GTCTAC GT^[AC][GT]AC
3203 GTAGAC
AcyI 2350 GGCGTC G[GA]^CG[CT]C
2701 GGCGTC
AflIII 1653 ACGTGT A^C[GA][CT]GT
1776 ACGTGT
AocI 1499 CCTCAGG CC^T[ACGT]AGG
3812 CCTCAGG
AspI 1361 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3982 GACCAGGTC
AviII 804 TGCGCA TGC^GCA
2019 TGCGCA
BcgI 76 CGAAGGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1637 GCAAGCCTATCG
BclI 1143 TGATCA T^GATCA
3518 TGATCA
BsaHI 2350 GGCGTC G[GA]^CG[CT]C
2701 GGCGTC
BsmBI 1555 CGTCTC CGTCTC(1/5)
2046 GAGACG
BssSI 1067 CACGAG CACGAG(-5/-1)
2405 CACGAG
BstYI 616 GGATCC [GA]^GATC[CT]
1824 AGATCC
Bsu36I 1499 CCTCAGG CC^T[ACGT]AGG
3812 CCTCAGG
DraI 1246 TTTAAA TTT^AAA
1316 TTTAAA
DrdI 950 GACGAAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2471 GACATGCTCGTC
Eco81I 1499 CCTCAGG CC^T[ACGT]AGG
3812 CCTCAGG
EcoNI 947 CCTGACGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2311 CCTGCTGCAGG
FbaI 1143 TGATCA T^GATCA
3518 TGATCA
FspI 804 TGCGCA TGC^GCA
2019 TGCGCA
Hin1I 2350 GGCGTC G[GA]^CG[CT]C
2701 GGCGTC
KspI 2235 CCGCGG CCGC^GG
4535 CCGCGG
MflI 616 GGATCC [GA]^GATC[CT]
1824 AGATCC
NaeI 2347 GCCGGC GCC^GGC
2750 GCCGGC
NgoMIV 2347 GCCGGC G^CCGGC
2750 GCCGGC
PflFI 1361 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3982 GACCAGGTC
SacII 2235 CCGCGG CCGC^GG
4535 CCGCGG
Tth111I 1361 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3982 GACCAGGTC
XcmI 3062 CCATGCTCCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3181 CCATGGGGGTAGTGG
XhoII 616 GGATCC [GA]^GATC[CT]
1824 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspEI
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MroI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I