##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AgeI        
AhdI        Aor51HI     AscI        AspEI       AvrII       
BbeI        BbrPI       BbvCI       BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaHI       BseAI       
BsiEI       BsmBI       BspDI       BspEI       BssHII      
BssSI       CelII       ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      FauI        
FseI        Hin1I       KasI        KspI        MfeI        
MluI        MroI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        SfiI        SfoI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       2155  GGTACC               G^GTACC
AflII          31  CTTAAG               C^TTAAG
Alw44I       2205  GTGCAC               G^TGCAC
ApaI         2575  GGGCCC               GGGCC^C
ApaLI        2205  GTGCAC               G^TGCAC
Asp718I      2155  GGTACC               G^GTACC
AspHI        2205  GTGCAC               G[AT]GC[AT]^C
AspI         3251  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         121  TGCGCA               TGC^GCA
BanI         2155  GGTACC               G^G[CT][GA]CC
BcnI           20  CCCGG                CC^[GC]GG
BfrI           31  CTTAAG               C^TTAAG
BsaBI         989  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1842  ACCGGA               [AT]^CCGG[AT]
BsiHKAI      2205  GTGCAC               G[AT]GC[AT]^C
BsiWI        1807  CGTACG               C^GTACG
BsmI         2547  GAATGC               GAATGC(1/-1)
BsrBI          65  GAGCGG               CCGCTC(-3/-3)
BsrFI        4510  ACCGGC               [GA]^CCGG[CT]
Bst1107I     4202  GTATAC               GTA^TAC
BstEII       3761  GGTTACC              G^GT[ACGT]ACC
BstPI        3761  GGTTACC              G^GT[ACGT]ACC
BstZ17I      4202  GTATAC               GTA^TAC
Cfr10I       4510  ACCGGC               [GA]^CCGG[CT]
DrdI         4593  GACATGGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      3761  GGTTACC              G^GT[ACGT]ACC
EcoRI        3137  GAATTC               G^AATTC
EcoRV        2174  GATATC               GAT^ATC
EcoT22I      4217  ATGCAT               ATGCA^T
FspI          121  TGCGCA               TGC^GCA
HgaI         2740  GCGTC                GACGC(5/10)
HindIII      3532  AAGCTT               A^AGCTT
HpaI         4691  GTTAAC               GTT^AAC
KpnI         2155  GGTACC               GGTAC^C
MamI          989  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI           20  CCCGG                CC^[GC]GG
NruI         3820  TCGCGA               TCG^CGA
NsiI         4217  ATGCAT               ATGCA^T
PflFI        3251  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI         2901  CTGCAG               CTGCA^G
SphI         1905  GCATGC               GCATG^C
SspI         1418  AATATT               AAT^ATT
StuI          372  AGGCCT               AGG^CCT
Tth111I      3251  GACTTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI          584  GTAGAC               GT^[AC][GT]AC
             4202  GTATAC              
AvaI         1191  CTCGGG               C^[CT]CG[GA]G
             1707  CCCGAG              
BamHI         730  GGATCC               G^GATCC
             1996  GGATCC              
BcgI         2207  GCACATTATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3811  GCATCTTATTCG        
BciVI        1387  GTATCC               GTATCC(6/5)
             4028  GTATCC              
BclI          559  TGATCA               T^GATCA
             4798  TGATCA              
BglII        3062  AGATCT               A^GATCT
             3176  AGATCT              
BsoBI        1191  CTCGGG               C^[CT]CG[GA]G
             1707  CCCGAG              
Bsp1407I     3744  TGTACA               T^GTACA
             4212  TGTACA              
BspLU11I     3747  ACATGT               A^CATGT
             4209  ACATGT              
BsrDI         563  CATTGC               GCAATG(2/0)
             2979  CATTGC              
BsrGI        3744  TGTACA               T^GTACA
             4212  TGTACA              
BstAPI        113  GCAGCCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3589  GCAGCTCTTGC         
BstBI        2215  TTCGAA               TT^CGAA
             3159  TTCGAA              
BstXI          82  CCACTGGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3942  CCAGGGCAGTGG        
DraIII       1241  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             4802  CACTGAGTG           
DsaI          108  CCATGG               C^C[GA][CT]GG
             1589  CCATGG              
EcoNI        2582  CCTGGTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4580  CCTTCTAAAGG         
FbaI          559  TGATCA               T^GATCA
             4798  TGATCA              
NcoI          108  CCATGG               C^CATGG
             1589  CCATGG              
NspV         2215  TTCGAA               TT^CGAA
             3159  TTCGAA              
PpuMI        2193  GGGACCC              [GA]G^G[AT]CC[CT]
             2470  GGGTCCT             
PvuII         644  CAGCTG               CAG^CTG
             4381  CAGCTG              
ScaI         1434  AGTACT               AGT^ACT
             3441  AGTACT              
SfuI         2215  TTCGAA               TT^CGAA
             3159  TTCGAA              
SspBI        3744  TGTACA               T^GTACA
             4212  TGTACA              
XcmI          129  CCAAGAAGATCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4479  CCACCCCTACTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        ApoI        
AseI        AsnI        Asp700I     AvaII       BalI        
BanII       BbsI        BbvI        BfaI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspHI       BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       EaeI        EarI        Eco81I      
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PshBI       
RcaI        RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XbaI        XhoII       XmnI