##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AccIII AgeI AscI AspI
AviII AvrII BbvCI BciVI BlnI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstZ17I ClaI DrdI EcoRI EcoRV
FseI FspI HindIII MluI MroI
NheI NotI NspV PacI PflFI
PinAI PmeI SalI SbfI ScaI
SfiI SfuI SgrAI SnaBI SphI
Sse8387I SspBI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AclI 2010 AACGTT AA^CGTT
AflII 5181 CTTAAG C^TTAAG
AseI 3869 ATTAAT AT^TAAT
AsnI 3869 ATTAAT AT^TAAT
BamHI 2576 GGATCC G^GATCC
BbrPI 5681 CACGTG CAC^GTG
BbsI 2838 GTCTTC GAAGAC(2/6)
BcgI 3144 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 5181 CTTAAG C^TTAAG
BpuAI 2838 GTCTTC GAAGAC(2/6)
BsaAI 5681 CACGTG [CT]AC^GT[GA]
BsaWI 3263 ACCGGA [AT]^CCGG[AT]
BspHI 4951 TCATGA T^CATGA
BssHII 1186 GCGCGC G^CGCGC
BstAPI 5786 GCATTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 1178 CGGACCG CG^G[AT]CCG
EcoT22I 2033 ATGCAT ATGCA^T
HpaI 4054 GTTAAC GTT^AAC
MfeI 3559 CAATTG C^AATTG
MunI 3559 CAATTG C^AATTG
NaeI 260 GCCGGC GCC^GGC
NdeI 3672 CATATG CA^TATG
NgoMIV 260 GCCGGC G^CCGGC
NsiI 2033 ATGCAT ATGCA^T
PaeR7I 2216 CTCGAG C^TCGAG
PmaCI 5681 CACGTG CAC^GTG
PmlI 5681 CACGTG CAC^GTG
PshAI 3318 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3869 ATTAAT AT^TAAT
Psp1406I 2010 AACGTT AA^CGTT
PvuI 2253 CGATCG CGAT^CG
RcaI 4951 TCATGA T^CATGA
RsrII 1178 CGGACCG CG^G[AT]CCG
SacI 5421 GAGCTC GAGCT^C
SapI 3540 GCTCTTC GCTCTTC(1/4)
SexAI 6282 ACCAGGT A^CC[AT]GGT
SpeI 3936 ACTAGT A^CTAGT
SwaI 4715 ATTTAAAT ATTT^AAAT
XcmI 4929 CCACTCCAACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2216 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
Acc65I 3260 GGTACC G^GTACC
5844 GGTACC
Alw44I 1876 GTGCAC G^TGCAC
5494 GTGCAC
AocI 13 CCTAAGG CC^T[ACGT]AGG
5774 CCTTAGG
Aor51HI 1329 AGCGCT AGC^GCT
3336 AGCGCT
ApaLI 1876 GTGCAC G^TGCAC
5494 GTGCAC
Asp700I 3697 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6218 GAAGTGCTTC
Asp718I 3260 GGTACC G^GTACC
5844 GGTACC
BclI 1766 TGATCA T^GATCA
3863 TGATCA
BglII 3344 AGATCT A^GATCT
5536 AGATCT
BsaBI 1689 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1761 GATTGTGATC
BsmBI 1866 CGTCTC CGTCTC(1/5)
3183 CGTCTC
BsrDI 2277 CATTGC GCAATG(2/0)
6818 GCAATG
BstEII 2913 GGTAACC G^GT[ACGT]ACC
3437 GGTTACC
BstPI 2913 GGTAACC G^GT[ACGT]ACC
3437 GGTTACC
BstXI 92 CCAGGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
194 CCACGTCCCTGG
Bsu36I 13 CCTAAGG CC^T[ACGT]AGG
5774 CCTTAGG
Eco47III 1329 AGCGCT AGC^GCT
3336 AGCGCT
Eco81I 13 CCTAAGG CC^T[ACGT]AGG
5774 CCTTAGG
EcoO65I 2913 GGTAACC G^GT[ACGT]ACC
3437 GGTTACC
FbaI 1766 TGATCA T^GATCA
3863 TGATCA
KpnI 3260 GGTACC GGTAC^C
5844 GGTACC
MamI 1689 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1761 GATTGTGATC
NruI 573 TCGCGA TCG^CGA
869 TCGCGA
PflMI 2282 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3906 CCACATCATGG
SspI 1951 AATATT AAT^ATT
4386 AATATT
Van91I 2282 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3906 CCACATCATGG
XmnI 3697 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6218 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI AlwI
AlwNI ApaI ApoI AspEI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EagI
Eam1105I EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI