##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AclI AcsI AflII AhdI ApoI
AseI AsnI Asp700I AspEI BciVI
BclI BfrI BsaBI BsiWI BsmI
BspHI Bst1107I BstBI BstZ17I CpoI
DraI Eam1105I EcoRI EcoRV EcoT22I
FbaI HindIII HpaI KspI MamI
MluI NdeI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII ScaI SexAI SfuI SgrAI
SnaBI SpeI SspI SwaI TfiI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1374 GACGTC GACGT^C
AgeI 4864 ACCGGT A^CCGGT
AscI 3217 GGCGCGCC GG^CGCGCC
AspI 2469 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2092 TGCGCA TGC^GCA
AvrII 4936 CCTAGG C^CTAGG
BamHI 4159 GGATCC G^GATCC
BbeI 396 GGCGCC GGCGC^C
BbrPI 1496 CACGTG CAC^GTG
BbvCI 864 GCTGAGG CCTCAGC(-5/-2)
BglII 5046 AGATCT A^GATCT
BlnI 4936 CCTAGG C^CTAGG
BsaAI 1496 CACGTG [CT]AC^GT[GA]
BsmBI 534 GAGACG CGTCTC(1/5)
BspDI 2310 ATCGAT AT^CGAT
BspLU11I 2374 ACATGT A^CATGT
BssHII 3218 GCGCGC G^CGCGC
ClaI 2310 ATCGAT AT^CGAT
DrdI 2466 GACGACTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 73 CGGCCG C^GGCCG
EclXI 73 CGGCCG C^GGCCG
Eco52I 73 CGGCCG C^GGCCG
FseI 986 GGCCGGCC GGCCGG^CC
FspI 2092 TGCGCA TGC^GCA
KasI 396 GGCGCC G^GCGCC
MfeI 639 CAATTG C^AATTG
MunI 639 CAATTG C^AATTG
NarI 396 GGCGCC GG^CGCC
NheI 4673 GCTAGC G^CTAGC
PflFI 2469 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4864 ACCGGT A^CCGGT
PmaCI 1496 CACGTG CAC^GTG
PmlI 1496 CACGTG CAC^GTG
SalI 2651 GTCGAC G^TCGAC
SfoI 396 GGCGCC GGC^GCC
Tsp509I 640 AATT ^AATT
Tth111I 2469 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 2178 GGTACC G^GTACC
2491 GGTACC
AccI 2651 GTCGAC GT^[AC][GT]AC
3291 GTCTAC
AccIII 508 TCCGGA T^CCGGA
790 TCCGGA
AflIII 1495 ACACGT A^C[GA][CT]GT
2374 ACATGT
Asp718I 2178 GGTACC G^GTACC
2491 GGTACC
BsaI 625 GAGACC GGTCTC(1/5)
4133 GGTCTC
BseAI 508 TCCGGA T^CCGGA
790 TCCGGA
Bsp1407I 2200 TGTACA T^GTACA
2995 TGTACA
BspEI 508 TCCGGA T^CCGGA
790 TCCGGA
BsrGI 2200 TGTACA T^GTACA
2995 TGTACA
BstEII 4298 GGTGACC G^GT[ACGT]ACC
4571 GGTGACC
BstPI 4298 GGTGACC G^GT[ACGT]ACC
4571 GGTGACC
EcoO65I 4298 GGTGACC G^GT[ACGT]ACC
4571 GGTGACC
HincII 1377 GTCAAC GT[CT]^[GA]AC
2651 GTCGAC
HindII 1377 GTCAAC GT[CT]^[GA]AC
2651 GTCGAC
KpnI 2178 GGTACC GGTAC^C
2491 GGTACC
MroI 508 TCCGGA T^CCGGA
790 TCCGGA
MseI 5068 TTAA T^TAA
5083 TTAA
SphI 1142 GCATGC GCATG^C
3835 GCATGC
SspBI 2200 TGTACA T^GTACA
2995 TGTACA
Tru9I 5068 TTAA T^TAA
5083 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SfiI SmaI SmlI Sse8387I
StuI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI